ISC1_VERDV
ID ISC1_VERDV Reviewed; 190 AA.
AC G2X4M1;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 1.
DT 25-MAY-2022, entry version 34.
DE RecName: Full=Secreted isochorismatase effector Isc1 {ECO:0000303|PubMed:25156390};
DE EC=3.3.2.1 {ECO:0000269|PubMed:25156390};
GN Name=Isc1 {ECO:0000303|PubMed:25156390}; ORFNames=VDAG_05103;
OS Verticillium dahliae (strain VdLs.17 / ATCC MYA-4575 / FGSC 10137)
OS (Verticillium wilt).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Glomerellales; Plectosphaerellaceae; Verticillium.
OX NCBI_TaxID=498257;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VdLs.17 / ATCC MYA-4575 / FGSC 10137;
RX PubMed=21829347; DOI=10.1371/journal.ppat.1002137;
RA Klosterman S.J., Subbarao K.V., Kang S., Veronese P., Gold S.E.,
RA Thomma B.P.H.J., Chen Z., Henrissat B., Lee Y.-H., Park J.,
RA Garcia-Pedrajas M.D., Barbara D.J., Anchieta A., de Jonge R., Santhanam P.,
RA Maruthachalam K., Atallah Z., Amyotte S.G., Paz Z., Inderbitzin P.,
RA Hayes R.J., Heiman D.I., Young S., Zeng Q., Engels R., Galagan J.,
RA Cuomo C.A., Dobinson K.F., Ma L.-J.;
RT "Comparative genomics yields insights into niche adaptation of plant
RT vascular wilt pathogens.";
RL PLoS Pathog. 7:E1002137-E1002137(2011).
RN [2]
RP INDUCTION, DISRUPTION PHENOTYPE, FUNCTION, ACTIVE SITE, MUTAGENESIS OF
RP ASP-26; LYS-100 AND CYS-133, CATALYTIC ACTIVITY, AND SUBCELLULAR LOCATION.
RX PubMed=25156390; DOI=10.1038/ncomms5686;
RA Liu T., Song T., Zhang X., Yuan H., Su L., Li W., Xu J., Liu S., Chen L.,
RA Chen T., Zhang M., Gu L., Zhang B., Dou D.;
RT "Unconventionally secreted effectors of two filamentous pathogens target
RT plant salicylate biosynthesis.";
RL Nat. Commun. 5:4686-4686(2014).
CC -!- FUNCTION: Secreted isochorismatase required for full virulence of
CC V.dahliae (PubMed:25156390). Suppresses salicylate-mediated innate
CC immunity of the host by disrupting the plant salicylate metabolism
CC pathway via hydrolysis of its isochorismate precursor
CC (PubMed:25156390). {ECO:0000269|PubMed:25156390}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + isochorismate = (2S,3S)-2,3-dihydroxy-2,3-
CC dihydrobenzoate + pyruvate; Xref=Rhea:RHEA:11112, ChEBI:CHEBI:15361,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:29780, ChEBI:CHEBI:58764; EC=3.3.2.1;
CC Evidence={ECO:0000269|PubMed:25156390};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:25156390}. Host
CC cytoplasm {ECO:0000269|PubMed:25156390}. Host nucleus
CC {ECO:0000269|PubMed:25156390}. Note=Lacks a signal peptide and uses an
CC unconventional secretion pathway for delivering effectors which plays
CC an important role in host-pathogen interactions.
CC {ECO:0000269|PubMed:25156390}.
CC -!- INDUCTION: Expression is up-regulated during the infection stages.
CC {ECO:0000269|PubMed:25156390}.
CC -!- DISRUPTION PHENOTYPE: Leads to a significant reduction in virulence and
CC increased the concentrations of both free salicylate and its glucoside
CC (SAG) by 100-150% in inoculated cotton plants.
CC {ECO:0000269|PubMed:25156390}.
CC -!- SIMILARITY: Belongs to the isochorismatase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DS572703; EGY23665.1; -; Genomic_DNA.
DR RefSeq; XP_009653134.1; XM_009654839.1.
DR AlphaFoldDB; G2X4M1; -.
DR SMR; G2X4M1; -.
DR STRING; 27337.EGY23665; -.
DR EnsemblFungi; EGY23665; EGY23665; VDAG_05103.
DR GeneID; 20706566; -.
DR KEGG; vda:VDAG_05103; -.
DR eggNOG; ENOG502RXWW; Eukaryota.
DR HOGENOM; CLU_068979_5_1_1; -.
DR InParanoid; G2X4M1; -.
DR OMA; TGMMTHM; -.
DR Proteomes; UP000001611; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0008908; F:isochorismatase activity; IDA:UniProtKB.
DR GO; GO:0140403; P:effector-mediated suppression of host innate immune response; IDA:UniProtKB.
DR Gene3D; 3.40.50.850; -; 1.
DR InterPro; IPR000868; Isochorismatase-like.
DR InterPro; IPR036380; Isochorismatase-like_sf.
DR Pfam; PF00857; Isochorismatase; 1.
DR SUPFAM; SSF52499; SSF52499; 1.
PE 1: Evidence at protein level;
KW Host cytoplasm; Host nucleus; Hydrolase; Reference proteome; Secreted;
KW Virulence.
FT CHAIN 1..190
FT /note="Secreted isochorismatase effector Isc1"
FT /id="PRO_0000448093"
FT ACT_SITE 26
FT /evidence="ECO:0000269|PubMed:25156390"
FT ACT_SITE 100
FT /evidence="ECO:0000269|PubMed:25156390"
FT ACT_SITE 133
FT /evidence="ECO:0000269|PubMed:25156390"
FT MUTAGEN 26
FT /note="D->A: Impairs the isochorismatase activity and
FT reduces the virulence; when associated with A-100 and A-
FT 133."
FT /evidence="ECO:0000269|PubMed:25156390"
FT MUTAGEN 100
FT /note="K->A: Impairs the isochorismatase activity and
FT reduces the virulence; when associated with A-26 and A-
FT 133."
FT /evidence="ECO:0000269|PubMed:25156390"
FT MUTAGEN 133
FT /note="C->A: Impairs the isochorismatase activity and
FT reduces the virulence; when associated with A-26 and A-
FT 100."
FT /evidence="ECO:0000269|PubMed:25156390"
SQ SEQUENCE 190 AA; 20192 MW; B2E2FB94EC9A0448 CRC64;
MSSFRSMLGV PPSTASTQDS VLVIIDAQGE YAEGKLKISN IEASRPNISS LLEKYRAANA
PIVHVVHETP AGAPLFTQGT KLAEIFDELT PKEGEAVVTK HHPGSFADTN LQEILEKSGK
KKIVLVGYMA HVCVSTTARQ GAQRGWDVIV AEDAVGDRDI PGVDAAQLVK VALAEIADVF
GTLVSSKDIN
