ISC2A_MOUSE
ID ISC2A_MOUSE Reviewed; 206 AA.
AC P85094;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Isochorismatase domain-containing protein 2A;
GN Name=Isoc2a {ECO:0000312|MGI:MGI:3609243};
GN Synonyms=Isoc2 {ECO:0000312|MGI:MGI:3609243};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-178, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=16916647; DOI=10.1016/j.molcel.2006.06.026;
RA Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y.,
RA Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y.;
RT "Substrate and functional diversity of lysine acetylation revealed by a
RT proteomics survey.";
RL Mol. Cell 23:607-618(2006).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=17658461; DOI=10.1016/j.bbrc.2007.06.181;
RA Huang X., Shi Z., Wang W., Bai J., Chen Z., Xu J., Zhang D., Fu S.;
RT "Identification and characterization of a novel protein ISOC2 that
RT interacts with p16INK4a.";
RL Biochem. Biophys. Res. Commun. 361:287-293(2007).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-26; LYS-93 AND LYS-178, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-93; LYS-178; LYS-182 AND LYS-185,
RP AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=23576753; DOI=10.1073/pnas.1302961110;
RA Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B.,
RA Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.;
RT "Label-free quantitative proteomics of the lysine acetylome in mitochondria
RT identifies substrates of SIRT3 in metabolic pathways.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013).
CC -!- SUBUNIT: Interacts with CDKN2A. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC Note=Localizes to the nucleus in the presence of CDKN2A. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. Expressed predominantly in uterus,
CC stomach and urinary tract. {ECO:0000269|PubMed:17658461}.
CC -!- SIMILARITY: Belongs to the isochorismatase family. {ECO:0000255}.
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DR EMBL; AC157563; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS51978.1; -.
DR RefSeq; NP_001095068.1; NM_001101598.1.
DR AlphaFoldDB; P85094; -.
DR SMR; P85094; -.
DR BioGRID; 576866; 1.
DR STRING; 10090.ENSMUSP00000131514; -.
DR iPTMnet; P85094; -.
DR PhosphoSitePlus; P85094; -.
DR SwissPalm; P85094; -.
DR EPD; P85094; -.
DR jPOST; P85094; -.
DR MaxQB; P85094; -.
DR PaxDb; P85094; -.
DR PeptideAtlas; P85094; -.
DR PRIDE; P85094; -.
DR ProteomicsDB; 301674; -.
DR TopDownProteomics; P85094; -.
DR Ensembl; ENSMUST00000125249; ENSMUSP00000131514; ENSMUSG00000086784.
DR GeneID; 664994; -.
DR KEGG; mmu:664994; -.
DR UCSC; uc012ewz.1; mouse.
DR CTD; 664994; -.
DR MGI; MGI:3609243; Isoc2a.
DR VEuPathDB; HostDB:ENSMUSG00000086784; -.
DR eggNOG; KOG4044; Eukaryota.
DR GeneTree; ENSGT00390000006753; -.
DR HOGENOM; CLU_066901_0_1_1; -.
DR InParanoid; P85094; -.
DR OMA; LIVTEHY; -.
DR OrthoDB; 1100720at2759; -.
DR PhylomeDB; P85094; -.
DR TreeFam; TF313459; -.
DR BioGRID-ORCS; 664994; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Isoc2a; mouse.
DR PRO; PR:P85094; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P85094; protein.
DR Bgee; ENSMUSG00000086784; Expressed in adult mammalian kidney and 57 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0031648; P:protein destabilization; ISO:MGI.
DR Gene3D; 3.40.50.850; -; 1.
DR InterPro; IPR040318; ISOC2.
DR InterPro; IPR000868; Isochorismatase-like.
DR InterPro; IPR036380; Isochorismatase-like_sf.
DR PANTHER; PTHR14119:SF4; PTHR14119:SF4; 1.
DR Pfam; PF00857; Isochorismatase; 1.
DR SUPFAM; SSF52499; SSF52499; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..206
FT /note="Isochorismatase domain-containing protein 2A"
FT /id="PRO_0000282952"
FT MOD_RES 26
FT /note="N6-succinyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 93
FT /note="N6-acetyllysine; alternate"
FT /evidence="ECO:0007744|PubMed:23576753"
FT MOD_RES 93
FT /note="N6-succinyllysine; alternate"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 178
FT /note="N6-acetyllysine; alternate"
FT /evidence="ECO:0007744|PubMed:16916647,
FT ECO:0007744|PubMed:23576753"
FT MOD_RES 178
FT /note="N6-succinyllysine; alternate"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 182
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23576753"
FT MOD_RES 185
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23576753"
SQ SEQUENCE 206 AA; 22417 MW; 8EB7E8FEEAFE0C6F CRC64;
MAAARASLGR ILPESSILFL CDLQEKFRPS IAYFPQIVSV AARMLKVARL LDVPILLTEQ
YPEGLGPTVP ELGAQGIRPV SKTCFSMVPA LQKELDGRSQ LQSVLLCGIE TQACILNTAL
DLLHRGLQVH VVVDACSSRS QVDRLVALAR MRQSGAFLAT SESLILQLVR DASHPQFKEI
QKIIKEPVPD SGLLSLFQGQ SPLTSC
