ID   ISCA1_DICDI             Reviewed;         139 AA.
AC   Q54VS1;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Iron-sulfur cluster assembly 1 homolog, mitochondrial;
DE   AltName: Full=Iron-sulfur assembly protein isca1;
DE   Flags: Precursor;
GN   Name=isca1; ORFNames=DDB_G0280173;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Involved in the assembly of mitochondrial iron-sulfur
CC       proteins. Probably involved in the binding of an intermediate of Fe/S
CC       cluster assembly (By similarity). {ECO:0000250|UniProtKB:Q9BUE6}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q9BUE6}.
CC   -!- SIMILARITY: Belongs to the HesB/IscA family. {ECO:0000305}.
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DR   EMBL; AAFI02000035; EAL67313.1; -; Genomic_DNA.
DR   RefSeq; XP_641284.1; XM_636192.1.
DR   AlphaFoldDB; Q54VS1; -.
DR   SMR; Q54VS1; -.
DR   STRING; 44689.DDB0302403; -.
DR   PaxDb; Q54VS1; -.
DR   EnsemblProtists; EAL67313; EAL67313; DDB_G0280173.
DR   GeneID; 8622416; -.
DR   KEGG; ddi:DDB_G0280173; -.
DR   dictyBase; DDB_G0280173; isca1.
DR   eggNOG; KOG1120; Eukaryota.
DR   HOGENOM; CLU_069054_4_0_1; -.
DR   InParanoid; Q54VS1; -.
DR   OMA; YQLYFDE; -.
DR   PhylomeDB; Q54VS1; -.
DR   Reactome; R-DDI-1362409; Mitochondrial iron-sulfur cluster biogenesis.
DR   PRO; PR:Q54VS1; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; ISS:dictyBase.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IBA:GO_Central.
DR   GO; GO:0097428; P:protein maturation by iron-sulfur cluster transfer; IBA:GO_Central.
DR   Gene3D; 2.60.300.12; -; 1.
DR   InterPro; IPR000361; FeS_biogenesis.
DR   InterPro; IPR016092; FeS_cluster_insertion.
DR   InterPro; IPR017870; FeS_cluster_insertion_CS.
DR   InterPro; IPR035903; HesB-like_dom_sf.
DR   Pfam; PF01521; Fe-S_biosyn; 1.
DR   SUPFAM; SSF89360; SSF89360; 1.
DR   TIGRFAMs; TIGR00049; TIGR00049; 1.
DR   PROSITE; PS01152; HESB; 1.
PE   3: Inferred from homology;
KW   Iron; Iron-sulfur; Metal-binding; Mitochondrion; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..139
FT                   /note="Iron-sulfur cluster assembly 1 homolog,
FT                   mitochondrial"
FT                   /id="PRO_0000331541"
FT   BINDING         52
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAC8"
FT   BINDING         117
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAC8"
FT   BINDING         119
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAC8"
SQ   SEQUENCE   139 AA;  15390 MW;  D7BC42F9A9162E47 CRC64;
     MSISGVVKLG KPSKKAIFSM TDSALKRVKE IMNDKKIDNC IGLRLGIKER GCSGMSYTLD
     FATQKNKFDE TVVADKDINI IVDSKALLSV IGTEMDYIEE PIKKEFIFIN PNATNTCGCG
     ESFTTKDFSI PDLKLPKKN