ID   ISCA1_RAT               Reviewed;         129 AA.
AC   Q80W96;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Iron-sulfur cluster assembly 1 homolog, mitochondrial;
DE   AltName: Full=HESB-like domain-containing protein 2;
DE   AltName: Full=Iron-sulfur assembly protein IscA;
DE   Flags: Precursor;
GN   Name=Isca1; Synonyms=Hbld2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=15262227; DOI=10.1016/j.bbapap.2004.05.004;
RA   Cozar-Castellano I., del Valle Machargo M., Trujillo E., Arteaga M.F.,
RA   Gonzalez T., Martin-Vasallo P., Avila J.;
RT   "hIscA: a protein implicated in the biogenesis of iron-sulfur clusters.";
RL   Biochim. Biophys. Acta 1700:179-188(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Involved in the maturation of mitochondrial 4Fe-4S proteins
CC       functioning late in the iron-sulfur cluster assembly pathway. Probably
CC       involved in the binding of an intermediate of Fe/S cluster assembly.
CC       {ECO:0000250|UniProtKB:Q9BUE6}.
CC   -!- SUBUNIT: Interacts with CRY2, but not with CRY1 (in vitro).
CC       {ECO:0000250|UniProtKB:Q9BUE6}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q9BUE6}.
CC   -!- SIMILARITY: Belongs to the HesB/IscA family. {ECO:0000305}.
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DR   EMBL; AF508158; AAP29778.1; -; mRNA.
DR   EMBL; BC070929; AAH70929.1; -; mRNA.
DR   EMBL; BC078677; AAH78677.1; -; mRNA.
DR   RefSeq; NP_853657.1; NM_181626.3.
DR   AlphaFoldDB; Q80W96; -.
DR   SMR; Q80W96; -.
DR   STRING; 10116.ENSRNOP00000024781; -.
DR   PaxDb; Q80W96; -.
DR   PRIDE; Q80W96; -.
DR   Ensembl; ENSRNOT00000117636; ENSRNOP00000078686; ENSRNOG00000018343.
DR   GeneID; 290985; -.
DR   KEGG; rno:290985; -.
DR   UCSC; RGD:727792; rat.
DR   CTD; 81689; -.
DR   RGD; 727792; Isca1.
DR   eggNOG; KOG1120; Eukaryota.
DR   GeneTree; ENSGT00490000043385; -.
DR   InParanoid; Q80W96; -.
DR   OrthoDB; 1578799at2759; -.
DR   PhylomeDB; Q80W96; -.
DR   TreeFam; TF314956; -.
DR   Reactome; R-RNO-1362409; Mitochondrial iron-sulfur cluster biogenesis.
DR   PRO; PR:Q80W96; -.
DR   Proteomes; UP000002494; Chromosome 17.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0050886; P:endocrine process; NAS:RGD.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IBA:GO_Central.
DR   GO; GO:0097428; P:protein maturation by iron-sulfur cluster transfer; IBA:GO_Central.
DR   Gene3D; 2.60.300.12; -; 1.
DR   InterPro; IPR000361; FeS_biogenesis.
DR   InterPro; IPR016092; FeS_cluster_insertion.
DR   InterPro; IPR017870; FeS_cluster_insertion_CS.
DR   InterPro; IPR035903; HesB-like_dom_sf.
DR   Pfam; PF01521; Fe-S_biosyn; 1.
DR   SUPFAM; SSF89360; SSF89360; 1.
DR   TIGRFAMs; TIGR00049; TIGR00049; 1.
DR   PROSITE; PS01152; HESB; 1.
PE   2: Evidence at transcript level;
KW   Iron; Iron-sulfur; Metal-binding; Mitochondrion; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..12
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           13..129
FT                   /note="Iron-sulfur cluster assembly 1 homolog,
FT                   mitochondrial"
FT                   /id="PRO_0000042737"
FT   BINDING         57
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAC8"
FT   BINDING         121
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAC8"
FT   BINDING         123
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAC8"
SQ   SEQUENCE   129 AA;  14149 MW;  2C3693C453688233 CRC64;
     MSASLVRATV RAVSKRKLQP TRAALTLTPS AVNKIKQLLK DKPEHVGLKV GVRTRGCNGL
     SYSLEYTKTK GDADEEVIQD GVRVFIEKKA QLTLLGTEMD YVEDKLSSEF VFNNPNIKGT
     CGCGESFNV