APR_RAT
ID APR_RAT Reviewed; 105 AA.
AC Q5U777;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Phorbol-12-myristate-13-acetate-induced protein 1;
DE AltName: Full=Protein Noxa;
GN Name=Pmaip1; Synonyms=Noxa;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Embryonic brain;
RA Yakovlev A.G.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Promotes activation of caspases and apoptosis. Promotes
CC mitochondrial membrane changes and efflux of apoptogenic proteins from
CC the mitochondria. Contributes to p53/TP53-dependent apoptosis after
CC radiation exposure. Promotes proteasomal degradation of MCL1. Competes
CC with BAK1 and with BIM/BCL2L11 for binding to MCL1; can displace BAK1
CC and BIM/BCL2L11 from their binding sites (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with MCL1 (By similarity). Interacts with BCL2A1 (By
CC similarity). Interacts with BAX (By similarity). Interacts with BCL2L10
CC (By similarity). {ECO:0000250|UniProtKB:Q13794}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q13794}.
CC -!- DOMAIN: The BH3 motif is essential for pro-apoptotic activity.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PMAIP1 family. {ECO:0000305}.
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DR EMBL; AY788892; AAV51955.1; -; mRNA.
DR RefSeq; NP_001008386.1; NM_001008385.1.
DR AlphaFoldDB; Q5U777; -.
DR SMR; Q5U777; -.
DR ComplexPortal; CPX-306; MCL1:PMAIP1 complex.
DR STRING; 10116.ENSRNOP00000025361; -.
DR PhosphoSitePlus; Q5U777; -.
DR PaxDb; Q5U777; -.
DR GeneID; 492821; -.
DR KEGG; rno:492821; -.
DR UCSC; RGD:1359266; rat.
DR CTD; 5366; -.
DR RGD; 1359266; Pmaip1.
DR VEuPathDB; HostDB:ENSRNOG00000018770; -.
DR eggNOG; ENOG502SEAE; Eukaryota.
DR HOGENOM; CLU_2262862_0_0_1; -.
DR InParanoid; Q5U777; -.
DR OMA; YCTWSAP; -.
DR OrthoDB; 1636703at2759; -.
DR TreeFam; TF339379; -.
DR Reactome; R-RNO-111448; Activation of NOXA and translocation to mitochondria.
DR Reactome; R-RNO-111453; BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
DR PRO; PR:Q5U777; -.
DR Proteomes; UP000002494; Chromosome 18.
DR Bgee; ENSRNOG00000018770; Expressed in thymus and 18 other tissues.
DR Genevisible; Q5U777; RN.
DR GO; GO:0097136; C:Bcl-2 family protein complex; ISO:RGD.
DR GO; GO:0005829; C:cytosol; ISO:RGD.
DR GO; GO:0005739; C:mitochondrion; IDA:RGD.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; ISO:RGD.
DR GO; GO:0006915; P:apoptotic process; IEP:RGD.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISO:RGD.
DR GO; GO:0042149; P:cellular response to glucose starvation; ISO:RGD.
DR GO; GO:0071456; P:cellular response to hypoxia; ISO:RGD.
DR GO; GO:0051607; P:defense response to virus; ISO:RGD.
DR GO; GO:0044346; P:fibroblast apoptotic process; IEA:Ensembl.
DR GO; GO:0097193; P:intrinsic apoptotic signaling pathway; ISO:RGD.
DR GO; GO:0072332; P:intrinsic apoptotic signaling pathway by p53 class mediator; ISO:RGD.
DR GO; GO:0008630; P:intrinsic apoptotic signaling pathway in response to DNA damage; ISO:RGD.
DR GO; GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; ISO:RGD.
DR GO; GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; ISO:RGD.
DR GO; GO:0048147; P:negative regulation of fibroblast proliferation; ISO:RGD.
DR GO; GO:0010917; P:negative regulation of mitochondrial membrane potential; ISO:RGD.
DR GO; GO:0043065; P:positive regulation of apoptotic process; IMP:RGD.
DR GO; GO:0043280; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; ISO:RGD.
DR GO; GO:0043517; P:positive regulation of DNA damage response, signal transduction by p53 class mediator; ISO:RGD.
DR GO; GO:1902043; P:positive regulation of extrinsic apoptotic signaling pathway via death domain receptors; ISO:RGD.
DR GO; GO:2000271; P:positive regulation of fibroblast apoptotic process; IEA:Ensembl.
DR GO; GO:0010907; P:positive regulation of glucose metabolic process; ISO:RGD.
DR GO; GO:2001244; P:positive regulation of intrinsic apoptotic signaling pathway; ISO:RGD.
DR GO; GO:0043525; P:positive regulation of neuron apoptotic process; ISO:RGD.
DR GO; GO:1900740; P:positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; ISO:RGD.
DR GO; GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; ISO:RGD.
DR GO; GO:0010498; P:proteasomal protein catabolic process; ISO:RGD.
DR GO; GO:0072593; P:reactive oxygen species metabolic process; ISO:RGD.
DR GO; GO:0046902; P:regulation of mitochondrial membrane permeability; ISO:RGD.
DR GO; GO:0001836; P:release of cytochrome c from mitochondria; ISO:RGD.
DR GO; GO:0043331; P:response to dsRNA; ISO:RGD.
DR GO; GO:0009411; P:response to UV; ISO:RGD.
DR GO; GO:0010165; P:response to X-ray; ISO:RGD.
DR GO; GO:0043029; P:T cell homeostasis; ISO:RGD.
DR InterPro; IPR024140; Noxa.
DR PANTHER; PTHR14299; PTHR14299; 1.
DR Pfam; PF15150; PMAIP1; 2.
PE 3: Inferred from homology;
KW Apoptosis; Mitochondrion; Reference proteome; Repeat.
FT CHAIN 1..105
FT /note="Phorbol-12-myristate-13-acetate-induced protein 1"
FT /id="PRO_0000333231"
FT REGION 50..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 92..101
FT /note="Required for mitochondrial location"
FT /evidence="ECO:0000250"
FT MOTIF 27..35
FT /note="BH3 1"
FT MOTIF 80..88
FT /note="BH3 2"
SQ SEQUENCE 105 AA; 11901 MW; 76EF4EE17827890E CRC64;
MPGRKARRNA PLNPTRAELP PEFAAQLRKI GDKVYCTWSA PDITAVLAQM PGKKSRKSTM
RRSPSPTRVP ADLKDECDQL RRIGDKVNLR QKLLNFISKL FNLIT
