ID   ISCR_ECOHS              Reviewed;         162 AA.
AC   A8A337;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=HTH-type transcriptional regulator IscR {ECO:0000255|HAMAP-Rule:MF_01176};
GN   Name=iscR {ECO:0000255|HAMAP-Rule:MF_01176}; OrderedLocusNames=EcHS_A2682;
OS   Escherichia coli O9:H4 (strain HS).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=331112;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HS;
RX   PubMed=18676672; DOI=10.1128/jb.00619-08;
RA   Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA   Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA   Henderson I.R., Sperandio V., Ravel J.;
RT   "The pangenome structure of Escherichia coli: comparative genomic analysis
RT   of E. coli commensal and pathogenic isolates.";
RL   J. Bacteriol. 190:6881-6893(2008).
CC   -!- FUNCTION: Regulates the transcription of several operons and genes
CC       involved in the biogenesis of Fe-S clusters and Fe-S-containing
CC       proteins. {ECO:0000255|HAMAP-Rule:MF_01176}.
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01176};
CC       Note=Binds 1 [2Fe-2S] cluster. {ECO:0000255|HAMAP-Rule:MF_01176};
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DR   EMBL; CP000802; ABV06941.1; -; Genomic_DNA.
DR   RefSeq; WP_001241356.1; NC_009800.1.
DR   AlphaFoldDB; A8A337; -.
DR   SMR; A8A337; -.
DR   GeneID; 66673581; -.
DR   KEGG; ecx:EcHS_A2682; -.
DR   HOGENOM; CLU_107144_0_0_6; -.
DR   OMA; YGLTIMM; -.
DR   Proteomes; UP000001123; Chromosome.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_01176; HTH_type_IscR; 1.
DR   InterPro; IPR010242; TF_HTH_IscR.
DR   InterPro; IPR030489; TR_Rrf2-type_CS.
DR   InterPro; IPR000944; Tscrpt_reg_Rrf2.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR33221; PTHR33221; 1.
DR   Pfam; PF02082; Rrf2; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR02010; IscR; 1.
DR   TIGRFAMs; TIGR00738; rrf2_super; 1.
DR   PROSITE; PS01332; HTH_RRF2_1; 1.
DR   PROSITE; PS51197; HTH_RRF2_2; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Activator; DNA-binding; Iron; Iron-sulfur; Metal-binding;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..162
FT                   /note="HTH-type transcriptional regulator IscR"
FT                   /id="PRO_1000085416"
FT   DOMAIN          2..131
FT                   /note="HTH rrf2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   DNA_BIND        28..51
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         92
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         98
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         104
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
SQ   SEQUENCE   162 AA;  17349 MW;  DE828D553774E3BD CRC64;
     MRLTSKGRYA VTAMLDVALN SEAGPVPLAD ISERQGISLS YLEQLFSRLR KNGLVSSVRG
     PGGGYLLGKD ASSIAVGEVI SAVDESVDAT RCQGKGGCQG GDKCLTHALW RDLSDRLTGF
     LNNITLGELV NNQEVLDVSG RQHTHDAPRT RIQDAIDVKL RA