ID   ISCR_ECOK1              Reviewed;         162 AA.
AC   A1AE68;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=HTH-type transcriptional regulator IscR {ECO:0000255|HAMAP-Rule:MF_01176};
GN   Name=iscR {ECO:0000255|HAMAP-Rule:MF_01176}; OrderedLocusNames=Ecok1_24640;
GN   ORFNames=APECO1_3994;
OS   Escherichia coli O1:K1 / APEC.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=405955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17293413; DOI=10.1128/jb.01726-06;
RA   Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J.,
RA   Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.;
RT   "The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7
RT   shares strong similarities with human extraintestinal pathogenic E. coli
RT   genomes.";
RL   J. Bacteriol. 189:3228-3236(2007).
CC   -!- FUNCTION: Regulates the transcription of several operons and genes
CC       involved in the biogenesis of Fe-S clusters and Fe-S-containing
CC       proteins. {ECO:0000255|HAMAP-Rule:MF_01176}.
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01176};
CC       Note=Binds 1 [2Fe-2S] cluster. {ECO:0000255|HAMAP-Rule:MF_01176};
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DR   EMBL; CP000468; ABJ01958.1; -; Genomic_DNA.
DR   RefSeq; WP_001241357.1; NC_008563.1.
DR   AlphaFoldDB; A1AE68; -.
DR   SMR; A1AE68; -.
DR   EnsemblBacteria; ABJ01958; ABJ01958; APECO1_3994.
DR   GeneID; 60903810; -.
DR   KEGG; ecv:APECO1_3994; -.
DR   HOGENOM; CLU_107144_0_0_6; -.
DR   OMA; YGLTIMM; -.
DR   Proteomes; UP000008216; Chromosome.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_01176; HTH_type_IscR; 1.
DR   InterPro; IPR010242; TF_HTH_IscR.
DR   InterPro; IPR030489; TR_Rrf2-type_CS.
DR   InterPro; IPR000944; Tscrpt_reg_Rrf2.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR33221; PTHR33221; 1.
DR   Pfam; PF02082; Rrf2; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR02010; IscR; 1.
DR   TIGRFAMs; TIGR00738; rrf2_super; 1.
DR   PROSITE; PS01332; HTH_RRF2_1; 1.
DR   PROSITE; PS51197; HTH_RRF2_2; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Activator; DNA-binding; Iron; Iron-sulfur; Metal-binding;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..162
FT                   /note="HTH-type transcriptional regulator IscR"
FT                   /id="PRO_1000085417"
FT   DOMAIN          2..131
FT                   /note="HTH rrf2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   DNA_BIND        28..51
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   REGION          140..162
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         92
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         98
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         104
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
SQ   SEQUENCE   162 AA;  17337 MW;  DE82977C3774E3BD CRC64;
     MRLTSKGRYA VTAMLDVALN SEAGPVPLAD ISERQGISLS YLEQLFSRLR KNGLVSSVRG
     PGGGYLLGKD ASSIAVGEVI SAVDESVDAT RCQGKGGCQG GDKCLTHALW RDLSDRLTGF
     LNNITLGELV NNQEVLDVSG RQHTHDAPRT RTQDAIDVKL RA