ID   ISCR_PECAS              Reviewed;         164 AA.
AC   Q6D258;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=HTH-type transcriptional regulator IscR {ECO:0000255|HAMAP-Rule:MF_01176};
GN   Name=iscR {ECO:0000255|HAMAP-Rule:MF_01176}; OrderedLocusNames=ECA3238;
OS   Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia
OS   carotovora subsp. atroseptica).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=218491;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=15263089; DOI=10.1073/pnas.0402424101;
RA   Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J.,
RA   Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K.,
RA   Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J.,
RA   Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Norbertczak H.,
RA   Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S.,
RA   Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.;
RT   "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora
RT   subsp. atroseptica and characterization of virulence factors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004).
CC   -!- FUNCTION: Regulates the transcription of several operons and genes
CC       involved in the biogenesis of Fe-S clusters and Fe-S-containing
CC       proteins. {ECO:0000255|HAMAP-Rule:MF_01176}.
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01176};
CC       Note=Binds 1 [2Fe-2S] cluster. {ECO:0000255|HAMAP-Rule:MF_01176};
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DR   EMBL; BX950851; CAG76136.1; -; Genomic_DNA.
DR   RefSeq; WP_011094757.1; NC_004547.2.
DR   AlphaFoldDB; Q6D258; -.
DR   SMR; Q6D258; -.
DR   STRING; 218491.ECA3238; -.
DR   DNASU; 2881621; -.
DR   EnsemblBacteria; CAG76136; CAG76136; ECA3238.
DR   GeneID; 57209922; -.
DR   KEGG; eca:ECA3238; -.
DR   PATRIC; fig|218491.5.peg.3280; -.
DR   eggNOG; COG1959; Bacteria.
DR   HOGENOM; CLU_107144_0_0_6; -.
DR   OMA; YGLTIMM; -.
DR   OrthoDB; 1794390at2; -.
DR   Proteomes; UP000007966; Chromosome.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_01176; HTH_type_IscR; 1.
DR   InterPro; IPR010242; TF_HTH_IscR.
DR   InterPro; IPR030489; TR_Rrf2-type_CS.
DR   InterPro; IPR000944; Tscrpt_reg_Rrf2.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR33221; PTHR33221; 1.
DR   Pfam; PF02082; Rrf2; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR02010; IscR; 1.
DR   TIGRFAMs; TIGR00738; rrf2_super; 1.
DR   PROSITE; PS01332; HTH_RRF2_1; 1.
DR   PROSITE; PS51197; HTH_RRF2_2; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Activator; DNA-binding; Iron; Iron-sulfur; Metal-binding;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..164
FT                   /note="HTH-type transcriptional regulator IscR"
FT                   /id="PRO_0000268917"
FT   DOMAIN          2..131
FT                   /note="HTH rrf2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   DNA_BIND        28..51
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         92
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         98
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         104
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
SQ   SEQUENCE   164 AA;  18029 MW;  5105B49485D37046 CRC64;
     MRLTSKGRYA VTAMLDVALH SQEGPVPLAD ISERQGISLS YLEQLFSRLR KNGLVASVRG
     PGGGYLLGKN ANEIAVGMVI SAVDESVDAT RCQGRESCQG GDRCLTHTLW RDLSDRITDF
     LNNITLDELV NNKEVLNVAD RQDTDTRRTA NGRIQETINV NLRA