ID   ISCR_SHIFL              Reviewed;         162 AA.
AC   P0AGL1; P77484;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=HTH-type transcriptional regulator IscR {ECO:0000255|HAMAP-Rule:MF_01176};
GN   Name=iscR {ECO:0000255|HAMAP-Rule:MF_01176}; Synonyms=yfhP;
GN   OrderedLocusNames=SF2578, S2750;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Regulates the transcription of several operons and genes
CC       involved in the biogenesis of Fe-S clusters and Fe-S-containing
CC       proteins. {ECO:0000255|HAMAP-Rule:MF_01176}.
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01176};
CC       Note=Binds 1 [2Fe-2S] cluster. {ECO:0000255|HAMAP-Rule:MF_01176};
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DR   EMBL; AE005674; AAN44077.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP17902.1; -; Genomic_DNA.
DR   RefSeq; NP_708370.1; NC_004337.2.
DR   RefSeq; WP_001241357.1; NZ_WPGW01000021.1.
DR   AlphaFoldDB; P0AGL1; -.
DR   SMR; P0AGL1; -.
DR   STRING; 198214.SF2578; -.
DR   EnsemblBacteria; AAN44077; AAN44077; SF2578.
DR   EnsemblBacteria; AAP17902; AAP17902; S2750.
DR   GeneID; 1026947; -.
DR   GeneID; 60903810; -.
DR   KEGG; sfl:SF2578; -.
DR   KEGG; sfx:S2750; -.
DR   PATRIC; fig|198214.7.peg.3078; -.
DR   HOGENOM; CLU_107144_0_0_6; -.
DR   OMA; YGLTIMM; -.
DR   OrthoDB; 1794390at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_01176; HTH_type_IscR; 1.
DR   InterPro; IPR010242; TF_HTH_IscR.
DR   InterPro; IPR030489; TR_Rrf2-type_CS.
DR   InterPro; IPR000944; Tscrpt_reg_Rrf2.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR33221; PTHR33221; 1.
DR   Pfam; PF02082; Rrf2; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR02010; IscR; 1.
DR   TIGRFAMs; TIGR00738; rrf2_super; 1.
DR   PROSITE; PS01332; HTH_RRF2_1; 1.
DR   PROSITE; PS51197; HTH_RRF2_2; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Activator; DNA-binding; Iron; Iron-sulfur; Metal-binding;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..162
FT                   /note="HTH-type transcriptional regulator IscR"
FT                   /id="PRO_0000109563"
FT   DOMAIN          2..131
FT                   /note="HTH rrf2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   DNA_BIND        28..51
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   REGION          140..162
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         92
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         98
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         104
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
SQ   SEQUENCE   162 AA;  17337 MW;  DE82977C3774E3BD CRC64;
     MRLTSKGRYA VTAMLDVALN SEAGPVPLAD ISERQGISLS YLEQLFSRLR KNGLVSSVRG
     PGGGYLLGKD ASSIAVGEVI SAVDESVDAT RCQGKGGCQG GDKCLTHALW RDLSDRLTGF
     LNNITLGELV NNQEVLDVSG RQHTHDAPRT RTQDAIDVKL RA