ID   ISCR_SHISS              Reviewed;         162 AA.
AC   Q3YZ21;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=HTH-type transcriptional regulator IscR {ECO:0000255|HAMAP-Rule:MF_01176};
GN   Name=iscR {ECO:0000255|HAMAP-Rule:MF_01176}; OrderedLocusNames=SSON_2613;
OS   Shigella sonnei (strain Ss046).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=300269;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ss046;
RX   PubMed=16275786; DOI=10.1093/nar/gki954;
RA   Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J.,
RA   Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J.,
RA   Xu J., Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J.,
RA   Jin Q.;
RT   "Genome dynamics and diversity of Shigella species, the etiologic agents of
RT   bacillary dysentery.";
RL   Nucleic Acids Res. 33:6445-6458(2005).
CC   -!- FUNCTION: Regulates the transcription of several operons and genes
CC       involved in the biogenesis of Fe-S clusters and Fe-S-containing
CC       proteins. {ECO:0000255|HAMAP-Rule:MF_01176}.
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01176};
CC       Note=Binds 1 [2Fe-2S] cluster. {ECO:0000255|HAMAP-Rule:MF_01176};
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DR   EMBL; CP000038; AAZ89241.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q3YZ21; -.
DR   SMR; Q3YZ21; -.
DR   EnsemblBacteria; AAZ89241; AAZ89241; SSON_2613.
DR   KEGG; ssn:SSON_2613; -.
DR   HOGENOM; CLU_107144_0_0_6; -.
DR   OMA; YGLTIMM; -.
DR   Proteomes; UP000002529; Chromosome.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_01176; HTH_type_IscR; 1.
DR   InterPro; IPR010242; TF_HTH_IscR.
DR   InterPro; IPR030489; TR_Rrf2-type_CS.
DR   InterPro; IPR000944; Tscrpt_reg_Rrf2.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR33221; PTHR33221; 1.
DR   Pfam; PF02082; Rrf2; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR02010; IscR; 1.
DR   TIGRFAMs; TIGR00738; rrf2_super; 1.
DR   PROSITE; PS01332; HTH_RRF2_1; 1.
DR   PROSITE; PS51197; HTH_RRF2_2; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Activator; DNA-binding; Iron; Iron-sulfur; Metal-binding;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..162
FT                   /note="HTH-type transcriptional regulator IscR"
FT                   /id="PRO_0000268928"
FT   DOMAIN          2..131
FT                   /note="HTH rrf2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   DNA_BIND        28..51
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         92
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         98
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
FT   BINDING         104
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01176"
SQ   SEQUENCE   162 AA;  17349 MW;  DE828D553774E3BD CRC64;
     MRLTSKGRYA VTAMLDVALN SEAGPVPLAD ISERQGISLS YLEQLFSRLR KNGLVSSVRG
     PGGGYLLGKD ASSIAVGEVI SAVDESVDAT RCQGKGGCQG GDKCLTHALW RDLSDRLTGF
     LNNITLGELV NNQEVLDVSG RQHTHDAPRT RIQDAIDVKL RA