ISCU_ECO57
ID ISCU_ECO57 Reviewed; 128 AA.
AC P0ACD6; P77310;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Iron-sulfur cluster assembly scaffold protein IscU;
DE AltName: Full=Sulfur acceptor protein IscU;
GN Name=iscU; Synonyms=nifU; OrderedLocusNames=Z3796, ECs3395;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) IN COMPLEX WITH ISCS, FUNCTION,
RP INTERACTION WITH ISCS, SUBUNIT, AND MUTAGENESIS OF 1-MET--LYS-6;
RP 1-MET--GLU-12; GLU-5; ASP-9; TYR-11; GLU-98 AND LYS-103.
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=20404999; DOI=10.1371/journal.pbio.1000354;
RA Shi R., Proteau A., Villarroya M., Moukadiri I., Zhang L., Trempe J.F.,
RA Matte A., Armengod M.E., Cygler M.;
RT "Structural basis for Fe-S cluster assembly and tRNA thiolation mediated by
RT IscS protein-protein interactions.";
RL PLoS Biol. 8:E1000354-E1000354(2010).
CC -!- FUNCTION: A scaffold on which IscS assembles Fe-S clusters.
CC Subsequently gives the nascent cluster to other proteins. It is likely
CC that Fe-S cluster coordination is flexible as the role of this complex
CC is to build and then hand off Fe-S clusters (Probable).
CC {ECO:0000305|PubMed:20404999}.
CC -!- SUBUNIT: Forms a heterotetramer with IscS; each subunit of the IscS
CC dimer contacts an IscU monomer. Certain pairs of proteins can bind
CC simultaneously to IscS; IscS-IscU-CyaY and IscS-IscU-IscX complexes can
CC be isolated in vitro, but others (IscS-IscU-TusA) cannot. IscU can
CC displace TusA from IscS. {ECO:0000269|PubMed:20404999}.
CC -!- INTERACTION:
CC P0ACD6; P0A6B9: iscS; NbExp=5; IntAct=EBI-9011202, EBI-9011195;
CC -!- SIMILARITY: Belongs to the NifU family. {ECO:0000305}.
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DR EMBL; AE005174; AAG57643.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB36818.1; -; Genomic_DNA.
DR PIR; C91053; C91053.
DR PIR; G85897; G85897.
DR RefSeq; NP_311422.1; NC_002695.1.
DR RefSeq; WP_000331707.1; NZ_SWKA01000005.1.
DR PDB; 3LVL; X-ray; 3.00 A; A=2-128.
DR PDBsum; 3LVL; -.
DR AlphaFoldDB; P0ACD6; -.
DR BMRB; P0ACD6; -.
DR SMR; P0ACD6; -.
DR DIP; DIP-58575N; -.
DR IntAct; P0ACD6; 3.
DR STRING; 155864.EDL933_3692; -.
DR EnsemblBacteria; AAG57643; AAG57643; Z3796.
DR EnsemblBacteria; BAB36818; BAB36818; ECs_3395.
DR GeneID; 67416913; -.
DR GeneID; 915163; -.
DR KEGG; ece:Z3796; -.
DR KEGG; ecs:ECs_3395; -.
DR PATRIC; fig|386585.9.peg.3547; -.
DR eggNOG; COG0822; Bacteria.
DR HOGENOM; CLU_079283_5_0_6; -.
DR OMA; YKNPRNY; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR CDD; cd06664; IscU_like; 1.
DR InterPro; IPR011339; ISC_FeS_clus_asmbl_IscU.
DR InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR PANTHER; PTHR10093; PTHR10093; 1.
DR Pfam; PF01592; NifU_N; 1.
DR TIGRFAMs; TIGR01999; iscU; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome.
FT CHAIN 1..128
FT /note="Iron-sulfur cluster assembly scaffold protein IscU"
FT /id="PRO_0000166185"
FT MUTAGEN 1..12
FT /note="Missing: No longer binds IscS."
FT /evidence="ECO:0000269|PubMed:20404999"
FT MUTAGEN 1..6
FT /note="Missing: Partial loss of IscS binding."
FT /evidence="ECO:0000269|PubMed:20404999"
FT MUTAGEN 5
FT /note="E->L: Binds IscS."
FT /evidence="ECO:0000269|PubMed:20404999"
FT MUTAGEN 9
FT /note="D->R: Binds IscS."
FT /evidence="ECO:0000269|PubMed:20404999"
FT MUTAGEN 11
FT /note="Y->A: Binds IscS."
FT /evidence="ECO:0000269|PubMed:20404999"
FT MUTAGEN 98
FT /note="E->R: Binds IscS."
FT /evidence="ECO:0000269|PubMed:20404999"
FT MUTAGEN 103
FT /note="K->E: No longer binds IscS."
FT /evidence="ECO:0000269|PubMed:20404999"
FT HELIX 5..12
FT /evidence="ECO:0007829|PDB:3LVL"
FT STRAND 15..18
FT /evidence="ECO:0007829|PDB:3LVL"
FT STRAND 25..33
FT /evidence="ECO:0007829|PDB:3LVL"
FT TURN 35..37
FT /evidence="ECO:0007829|PDB:3LVL"
FT STRAND 40..47
FT /evidence="ECO:0007829|PDB:3LVL"
FT STRAND 49..51
FT /evidence="ECO:0007829|PDB:3LVL"
FT STRAND 53..62
FT /evidence="ECO:0007829|PDB:3LVL"
FT HELIX 64..77
FT /evidence="ECO:0007829|PDB:3LVL"
FT HELIX 82..86
FT /evidence="ECO:0007829|PDB:3LVL"
FT HELIX 90..97
FT /evidence="ECO:0007829|PDB:3LVL"
FT HELIX 101..104
FT /evidence="ECO:0007829|PDB:3LVL"
FT HELIX 105..123
FT /evidence="ECO:0007829|PDB:3LVL"
SQ SEQUENCE 128 AA; 13849 MW; 47516021442B6535 CRC64;
MAYSEKVIDH YENPRNVGSF DNNDENVGSG MVGAPACGDV MKLQIKVNDE GIIEDARFKT
YGCGSAIASS SLVTEWVKGK SLDEAQAIKN TDIAEELELP PVKIHCSILA EDAIKAAIAD
YKSKREAK
