ISCX_ECOLI
ID ISCX_ECOLI Reviewed; 66 AA.
AC P0C0L9; P37096;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Protein IscX;
GN Name=iscX; Synonyms=yfhJ; OrderedLocusNames=b2524, JW2508;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1317854; DOI=10.1016/s0021-9258(19)49883-9;
RA Ta D.T., Vickery L.E.;
RT "Cloning, sequencing, and overexpression of a [2Fe-2S] ferredoxin gene from
RT Escherichia coli.";
RL J. Biol. Chem. 267:11120-11125(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8300516; DOI=10.1128/jb.176.3.610-619.1994;
RA Kawula T.H., Lelivelt M.J.;
RT "Mutations in a gene encoding a new Hsp70 suppress rapid DNA inversion and
RT bgl activation, but not proU derepression, in hns-1 mutant Escherichia
RT coli.";
RL J. Bacteriol. 176:610-619(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 2-66, AND SUBUNIT.
RX PubMed=15937904; DOI=10.1002/prot.20481;
RA Shimomura Y., Takahashi Y., Kakuta Y., Fukuyama K.;
RT "Crystal structure of Escherichia coli YfhJ protein, a member of the ISC
RT machinery involved in assembly of iron-sulfur clusters.";
RL Proteins 60:566-569(2005).
CC -!- FUNCTION: May function as iron donor in the assembly of iron-sulfur
CC clusters.
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:15937904}.
CC -!- SIMILARITY: Belongs to the IscX family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M88654; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U01827; -; NOT_ANNOTATED_CDS; Unassigned_DNA.
DR EMBL; U00096; AAC75577.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16414.1; -; Genomic_DNA.
DR PIR; C65029; C65029.
DR RefSeq; NP_417019.1; NC_000913.3.
DR RefSeq; WP_000523616.1; NZ_STEB01000011.1.
DR PDB; 1UJ8; X-ray; 1.75 A; A=2-66.
DR PDB; 2BZT; NMR; -; A=1-66.
DR PDBsum; 1UJ8; -.
DR PDBsum; 2BZT; -.
DR AlphaFoldDB; P0C0L9; -.
DR BMRB; P0C0L9; -.
DR SMR; P0C0L9; -.
DR BioGRID; 4260583; 9.
DR DIP; DIP-48188N; -.
DR IntAct; P0C0L9; 5.
DR STRING; 511145.b2524; -.
DR jPOST; P0C0L9; -.
DR PaxDb; P0C0L9; -.
DR PRIDE; P0C0L9; -.
DR EnsemblBacteria; AAC75577; AAC75577; b2524.
DR EnsemblBacteria; BAA16414; BAA16414; BAA16414.
DR GeneID; 67416908; -.
DR GeneID; 945196; -.
DR KEGG; ecj:JW2508; -.
DR KEGG; eco:b2524; -.
DR PATRIC; fig|1411691.4.peg.4210; -.
DR EchoBASE; EB2217; -.
DR eggNOG; COG2975; Bacteria.
DR HOGENOM; CLU_168040_1_0_6; -.
DR InParanoid; P0C0L9; -.
DR OMA; AIQQAWI; -.
DR PhylomeDB; P0C0L9; -.
DR BioCyc; EcoCyc:EG12311-MON; -.
DR EvolutionaryTrace; P0C0L9; -.
DR PRO; PR:P0C0L9; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0004857; F:enzyme inhibitor activity; IDA:EcoCyc.
DR GO; GO:0008198; F:ferrous iron binding; IDA:EcoCyc.
DR GO; GO:0005506; F:iron ion binding; IDA:EcoCyc.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IBA:GO_Central.
DR Gene3D; 1.10.10.600; -; 1.
DR InterPro; IPR007479; ISC_FeS_clus_asmbl_IscsX.
DR InterPro; IPR036762; IscX-like_sf.
DR PANTHER; PTHR37532; PTHR37532; 1.
DR Pfam; PF04384; Fe-S_assembly; 1.
DR PIRSF; PIRSF039003; IscX; 1.
DR SUPFAM; SSF140319; SSF140319; 1.
DR TIGRFAMs; TIGR03412; iscX_yfhJ; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome.
FT CHAIN 1..66
FT /note="Protein IscX"
FT /id="PRO_0000211853"
FT CONFLICT 43..51
FT /note="DDDPQASNE -> RTDPGIPTK (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 55
FT /note="E -> R (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 58..66
FT /note="LLVWLDEAE -> C (in Ref. 2)"
FT /evidence="ECO:0000305"
FT HELIX 8..18
FT /evidence="ECO:0007829|PDB:1UJ8"
FT HELIX 24..26
FT /evidence="ECO:0007829|PDB:1UJ8"
FT HELIX 29..37
FT /evidence="ECO:0007829|PDB:1UJ8"
FT STRAND 40..42
FT /evidence="ECO:0007829|PDB:2BZT"
FT HELIX 46..48
FT /evidence="ECO:0007829|PDB:1UJ8"
FT HELIX 51..64
FT /evidence="ECO:0007829|PDB:1UJ8"
SQ SEQUENCE 66 AA; 7732 MW; 31C176F0C906AAD7 CRC64;
MGLKWTDSRE IGEALYDAYP DLDPKTVRFT DMHQWICDLE DFDDDPQASN EKILEAILLV
WLDEAE
