APTF1_CAEEL
ID APTF1_CAEEL Reviewed; 365 AA.
AC Q09585;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Transcription factor aptf-1 {ECO:0000303|PubMed:26949257};
DE AltName: Full=AP2 transcription factor aptf-1 {ECO:0000303|PubMed:24184105};
GN Name=aptf-1 {ECO:0000303|PubMed:24184105, ECO:0000312|WormBase:K06A1.1};
GN ORFNames=K06A1.1 {ECO:0000312|WormBase:K06A1.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|EMBL:CCD72609.1};
RN [1] {ECO:0000312|EMBL:CCD72609.1, ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:CCD72609.1,
RC ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, DISRUPTION
RP PHENOTYPE, AND PHYLOGENETIC ANALYSIS.
RX PubMed=24184105; DOI=10.1016/j.cub.2013.09.028;
RA Turek M., Lewandrowski I., Bringmann H.;
RT "An AP2 transcription factor is required for a sleep-active neuron to
RT induce sleep-like quiescence in C. elegans.";
RL Curr. Biol. 23:2215-2223(2013).
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26949257; DOI=10.7554/elife.12499;
RA Turek M., Besseling J., Spies J.P., Koenig S., Bringmann H.;
RT "Sleep-active neuron specification and sleep induction require FLP-11
RT neuropeptides to systemically induce sleep.";
RL Elife 5:0-0(2016).
CC -!- FUNCTION: Transcription factor, which is required in the single sleep-
CC active ring interneuron RIS for sleep-like behavioral quiescence
CC induced by neuropeptide signaling in larvae (PubMed:24184105,
CC PubMed:26949257). Regulates gene expression of sleep-inducing
CC FMRFamide-like neuropeptide flp-11 in RIS (PubMed:26949257).
CC {ECO:0000269|PubMed:24184105, ECO:0000269|PubMed:26949257}.
CC -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000250|UniProtKB:Q91ZK0}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q92754}.
CC -!- TISSUE SPECIFICITY: Expressed in five interneurons AIB, RIB and RIS.
CC {ECO:0000269|PubMed:24184105}.
CC -!- DEVELOPMENTAL STAGE: Expressed strongly during late embryogenesis and
CC early larval development in three types of interneurons, the paired
CC neurons AIB and RIB and the unpaired neuron RIS in the head, with the
CC strongest expression in RIS. Expression after embryonic development
CC decreases until the adult stage. {ECO:0000269|PubMed:24184105}.
CC -!- INDUCTION: Regulates its own expression during larval development
CC (PubMed:24184105). Transcriptionally regulated by transcription factor
CC lim-6 in RIS interneuron in all developmental stages (PubMed:26949257).
CC {ECO:0000269|PubMed:24184105, ECO:0000269|PubMed:26949257}.
CC -!- DISRUPTION PHENOTYPE: Animals develop with normal speed and go normally
CC through the molt. They stop feeding for a normal time before each molt,
CC but average nose speed is reduced by only about 10% and unlike wild-
CC type, they are never immobile. The command interneurons AVA and AVE,
CC which control backward movement, are not shut down during sleep-like
CC behavior. Optogenetic channelrhodopsin activation of AIBs, RIBs and RIS
CC interneurons during wake-like behavior using blue light does not
CC trigger acute immobility and cessation of pumping in L1 larvae, instead
CC it causes an increase in mobility (PubMed:24184105). No change in
CC GABAergic function of neurons as unc-25 and the vesicular GABA
CC transporter gene unc-47 are expressed normally. The expression of a
CC neuropeptide gene flp-11 is strongly down-regulated in pretzel-stage
CC embryos and in sleeping L4 larvae (PubMed:26949257).
CC {ECO:0000269|PubMed:24184105, ECO:0000269|PubMed:26949257}.
CC -!- SIMILARITY: Belongs to the AP-2 family. {ECO:0000305}.
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DR EMBL; BX284602; CCD72609.1; -; Genomic_DNA.
DR PIR; T34333; T34333.
DR RefSeq; NP_495300.2; NM_062899.2.
DR AlphaFoldDB; Q09585; -.
DR STRING; 6239.K06A1.1; -.
DR PaxDb; Q09585; -.
DR EnsemblMetazoa; K06A1.1.1; K06A1.1.1; WBGene00019424.
DR GeneID; 187043; -.
DR KEGG; cel:CELE_K06A1.1; -.
DR UCSC; K06A1.1; c. elegans.
DR CTD; 187043; -.
DR WormBase; K06A1.1; CE35990; WBGene00019424; aptf-1.
DR eggNOG; KOG3811; Eukaryota.
DR GeneTree; ENSGT00950000182848; -.
DR HOGENOM; CLU_804708_0_0_1; -.
DR InParanoid; Q09585; -.
DR OMA; MFNRKLM; -.
DR OrthoDB; 641707at2759; -.
DR PhylomeDB; Q09585; -.
DR Reactome; R-CEL-3232118; SUMOylation of transcription factors.
DR Reactome; R-CEL-8866904; Negative regulation of activity of TFAP2 (AP-2) family transcription factors.
DR Reactome; R-CEL-8866907; Activation of the TFAP2 (AP-2) family of transcription factors.
DR PRO; PR:Q09585; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00019424; Expressed in pharyngeal muscle cell (C elegans) and 2 other tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0042127; P:regulation of cell population proliferation; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0030431; P:sleep; IMP:WormBase.
DR InterPro; IPR004979; TF_AP2.
DR InterPro; IPR013854; TF_AP2_C.
DR PANTHER; PTHR10812; PTHR10812; 1.
DR Pfam; PF03299; TF_AP-2; 1.
DR PRINTS; PR01748; AP2TNSCPFCT.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..365
FT /note="Transcription factor aptf-1"
FT /id="PRO_0000437544"
FT REGION 13..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 93..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 223..356
FT /note="H-S-H (helix-span-helix), dimerization"
FT /evidence="ECO:0000255"
SQ SEQUENCE 365 AA; 41328 MW; 8E25A54BA9953E8A CRC64;
MFNRKLMECS DDEFVRGSQC DEEDEEEQQR QSSQRPPFYE NMRTFAPSHM HISCHYDIPY
PPHLIQPPVC GVSTFTYARE FPVSNSQQFF NVTPPQQQQQ QGTGGSGEED YCIPKSEDRD
HSSNYSQVAT KQDRPFVFFA SDCQSPLEIV GEVYCTVPGR TSLLSSTTKY RVTVAEIQRR
ISPPECLNAS LLGGILRKAK SKDGGKTLRD SLKKLGLTLP AGRRKQANVT AWTALVEEEA
IHMAKEFALV CEKEFHSREI GIYLTKTSLA IDPDVVKRRT ALEMSRKVVG ELAELLSCDR
TPLTPYFPRN MLPIDPSVQQ HLSHFTLMTH GFGNVAMSAV LESVKLMIDE SIKYIDRCCS
QNVWR
