ISDH_STAAM
ID ISDH_STAAM Reviewed; 891 AA.
AC Q931P4;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Iron-regulated surface determinant protein H;
DE AltName: Full=Haptoglobin receptor A;
DE AltName: Full=Staphylococcus aureus surface protein I;
DE Flags: Precursor;
GN Name=isdH; Synonyms=harA, sasI; OrderedLocusNames=SAV1731;
OS Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=158878;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mu50 / ATCC 700699;
RX PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA Hiramatsu K.;
RT "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL Lancet 357:1225-1240(2001).
CC -!- FUNCTION: Binds human plasma haptoglobin-hemoglobin complexes,
CC haptoglobin and hemoglobin. Binds haptoglobin-hemoglobin complexes with
CC significantly higher affinity than haptoglobin alone (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}; Peptidoglycan-
CC anchor {ECO:0000305}.
CC -!- DOMAIN: The NEAT 1 domain binds with higher affinity than the NEAT 2
CC domain haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the IsdH family. {ECO:0000305}.
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DR EMBL; BA000017; BAB57893.1; -; Genomic_DNA.
DR RefSeq; WP_001032804.1; NC_002758.2.
DR PDB; 2E7D; X-ray; 2.20 A; A/B=539-664.
DR PDB; 2Z6F; X-ray; 1.90 A; A=539-664.
DR PDB; 3QUG; X-ray; 1.70 A; A/B=539-664.
DR PDB; 3QUH; X-ray; 2.70 A; A/B=539-664.
DR PDB; 3VTM; X-ray; 2.80 A; A/B=543-655.
DR PDB; 3VUA; X-ray; 1.85 A; A/B/C/D/E/F=539-664.
DR PDBsum; 2E7D; -.
DR PDBsum; 2Z6F; -.
DR PDBsum; 3QUG; -.
DR PDBsum; 3QUH; -.
DR PDBsum; 3VTM; -.
DR PDBsum; 3VUA; -.
DR AlphaFoldDB; Q931P4; -.
DR SMR; Q931P4; -.
DR TCDB; 9.A.39.1.2; the gram-positive bacterial hemoglobin receptor (isd) family.
DR PaxDb; Q931P4; -.
DR EnsemblBacteria; BAB57893; BAB57893; SAV1731.
DR KEGG; sav:SAV1731; -.
DR HOGENOM; CLU_016167_1_0_9; -.
DR OMA; TDKGVDN; -.
DR BioCyc; SAUR158878:SAV_RS09300-MON; -.
DR EvolutionaryTrace; Q931P4; -.
DR Proteomes; UP000002481; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR CDD; cd06920; NEAT; 1.
DR Gene3D; 2.60.40.1850; -; 3.
DR InterPro; IPR019930; IsdH.
DR InterPro; IPR019931; LPXTG_anchor.
DR InterPro; IPR006635; NEAT_dom.
DR InterPro; IPR037250; NEAT_dom_sf.
DR InterPro; IPR005877; YSIRK_signal_dom.
DR Pfam; PF05031; NEAT; 3.
DR Pfam; PF04650; YSIRK_signal; 1.
DR SMART; SM00725; NEAT; 3.
DR SUPFAM; SSF158911; SSF158911; 3.
DR TIGRFAMs; TIGR03658; IsdH_HarA; 1.
DR TIGRFAMs; TIGR01168; YSIRK_signal; 1.
DR PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
DR PROSITE; PS50978; NEAT; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Cell wall; Peptidoglycan-anchor; Repeat; Secreted; Signal.
FT SIGNAL 1..40
FT /evidence="ECO:0000255"
FT CHAIN 41..860
FT /note="Iron-regulated surface determinant protein H"
FT /id="PRO_0000285187"
FT PROPEP 861..891
FT /note="Removed by sortase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT /id="PRO_0000285188"
FT DOMAIN 105..232
FT /note="NEAT 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00337"
FT DOMAIN 345..471
FT /note="NEAT 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00337"
FT DOMAIN 543..660
FT /note="NEAT 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00337"
FT REGION 42..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 239..324
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 657..718
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 752..777
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 835..864
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 857..861
FT /note="LPXTG sorting signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 657..698
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 700..718
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 757..772
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 847..864
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 860
FT /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT STRAND 545..550
FT /evidence="ECO:0007829|PDB:3QUG"
FT STRAND 552..562
FT /evidence="ECO:0007829|PDB:3QUG"
FT HELIX 564..567
FT /evidence="ECO:0007829|PDB:3QUG"
FT STRAND 571..578
FT /evidence="ECO:0007829|PDB:3QUG"
FT STRAND 581..590
FT /evidence="ECO:0007829|PDB:3QUG"
FT HELIX 591..593
FT /evidence="ECO:0007829|PDB:3QUG"
FT STRAND 594..599
FT /evidence="ECO:0007829|PDB:3QUG"
FT STRAND 605..610
FT /evidence="ECO:0007829|PDB:3QUG"
FT HELIX 611..613
FT /evidence="ECO:0007829|PDB:3QUG"
FT STRAND 615..621
FT /evidence="ECO:0007829|PDB:3QUG"
FT STRAND 628..637
FT /evidence="ECO:0007829|PDB:3QUG"
FT HELIX 638..640
FT /evidence="ECO:0007829|PDB:3QUG"
FT STRAND 642..652
FT /evidence="ECO:0007829|PDB:3QUG"
SQ SEQUENCE 891 AA; 100466 MW; 7226A157FFE27123 CRC64;
MNKHHPKLRS FYSIRKSTLG VASVIVSTLF LITSQHQAQA AENTNTSDKI SENQNNNATT
TQQPKDTNQT QPATQPVITA KNYPAADESL KDAIKDPALE NKEHDIGPRE QVNFQLLDKN
NETQYYHFFS IKDPADVYYT KKKAEVELDI NTASTWKKFE VYENNQKLPV RLVSYSPVPE
DHAYIRFPVS DGTQELKIVS STQIDDGEET NYDYTKLVFA KPIYNDPSLV KSDTNDAVVT
NDQSSSDASN QTNTNTSNQN TSTTNNANNQ PQATTNMSQP AQPKSSANAD QASSQPAHET
NSNGNTNDKT NESSNQSDVN QQYPPADESL QDAIKNPAII DKEHTADNWR PIDFQMKNDK
GERQFYHYAS TVEPATVIFT KTGPVIELGL KTASTWKKFE VYEGDKKLPV ELVSYDSDKD
YAYIRFPVSN GTRDVKIVSS IEYGENIHED YDYTLMVFAQ PITNNPDDYV DEETYNLQKL
LAPYHKAKTL ERQVYELEKL QEKLPEKYKA EYKKKLDQTR VELADQVKSA VTEFENVTPT
NDQLTDLQEA HFVVFESEEN SESVMDGFVE HPFYTATLNG QKYVVMKTKD DSYWKDLIVE
GKRVTTVSKD PKNNSRTLIF PYIPDKAVYN AIVKVVVANI GYEGQYHVRI INQDINTKDD
DTSQNNTSEP LNVQTGQEGK VADTDVAENS STATNPKDAS DKADVIEPES DVVKDADNNI
DKDVQHDVDH LSDMSDNNHF DKYDLKEMDT QIAKDTDRNV DNSVGMSSNV DTDKDSNKNK
DKVIQLAHIA DKNNHTGKAA KLDVVKQNYN NTDKVTDKKT TEHLPSDIHK TVDKTVKTKE
KAGTPSKENK LSQSKMLPKT GETTSSQSWW GLYALLGMLA LFIPKFRKES K
