ISDH_STAAS
ID ISDH_STAAS Reviewed; 895 AA.
AC Q6G8J7;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=Iron-regulated surface determinant protein H;
DE AltName: Full=Haptoglobin receptor A;
DE AltName: Full=Staphylococcus aureus surface protein I;
DE Flags: Precursor;
GN Name=isdH; Synonyms=harA, sasI; OrderedLocusNames=SAS1657;
OS Staphylococcus aureus (strain MSSA476).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=282459;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MSSA476;
RX PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT for the rapid evolution of virulence and drug resistance.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
RN [2]
RP STRUCTURE BY NMR OF 86-229, AND BINDING TO HEMOGLOBIN.
RX PubMed=16762363; DOI=10.1016/j.jmb.2006.05.019;
RA Pilpa R.M., Fadeev E.A., Villareal V.A., Wong M.L., Phillips M.,
RA Clubb R.T.;
RT "Solution structure of the NEAT (NEAr Transporter) domain from IsdH/HarA:
RT the human hemoglobin receptor in Staphylococcus aureus.";
RL J. Mol. Biol. 360:435-447(2006).
CC -!- FUNCTION: Binds human plasma haptoglobin-hemoglobin complexes,
CC haptoglobin and hemoglobin. Binds haptoglobin-hemoglobin complexes with
CC significantly higher affinity than haptoglobin alone (Probable).
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}; Peptidoglycan-
CC anchor {ECO:0000305}.
CC -!- DOMAIN: The NEAT 1 domain binds with higher affinity than the NEAT 2
CC domain haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin (By
CC similarity). More than one IsdH NEAT 1 domain interacts with a single
CC hemoglobin. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the IsdH family. {ECO:0000305}.
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DR EMBL; BX571857; CAG43459.1; -; Genomic_DNA.
DR RefSeq; WP_001032759.1; NC_002953.3.
DR PDB; 2H3K; NMR; -; A=86-229.
DR PDB; 3OVU; X-ray; 2.83 A; B=86-229.
DR PDB; 3S48; X-ray; 3.05 A; A/B=86-229.
DR PDBsum; 2H3K; -.
DR PDBsum; 3OVU; -.
DR PDBsum; 3S48; -.
DR AlphaFoldDB; Q6G8J7; -.
DR BMRB; Q6G8J7; -.
DR SMR; Q6G8J7; -.
DR KEGG; sas:SAS1657; -.
DR HOGENOM; CLU_016167_1_0_9; -.
DR OMA; TDKGVDN; -.
DR EvolutionaryTrace; Q6G8J7; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR CDD; cd06920; NEAT; 1.
DR Gene3D; 2.60.40.1850; -; 3.
DR InterPro; IPR019930; IsdH.
DR InterPro; IPR019931; LPXTG_anchor.
DR InterPro; IPR006635; NEAT_dom.
DR InterPro; IPR037250; NEAT_dom_sf.
DR InterPro; IPR005877; YSIRK_signal_dom.
DR Pfam; PF05031; NEAT; 3.
DR SMART; SM00725; NEAT; 3.
DR SUPFAM; SSF158911; SSF158911; 3.
DR TIGRFAMs; TIGR03658; IsdH_HarA; 1.
DR TIGRFAMs; TIGR01168; YSIRK_signal; 1.
DR PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
DR PROSITE; PS50978; NEAT; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Cell wall; Peptidoglycan-anchor; Repeat; Secreted; Signal.
FT SIGNAL 1..40
FT /evidence="ECO:0000255"
FT CHAIN 41..864
FT /note="Iron-regulated surface determinant protein H"
FT /id="PRO_0000285185"
FT PROPEP 865..895
FT /note="Removed by sortase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT /id="PRO_0000285186"
FT DOMAIN 105..232
FT /note="NEAT 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00337"
FT DOMAIN 345..471
FT /note="NEAT 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00337"
FT DOMAIN 543..660
FT /note="NEAT 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00337"
FT REGION 42..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 239..324
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 657..705
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 752..782
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 841..868
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 861..865
FT /note="LPXTG sorting signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT COMPBIAS 42..82
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 657..698
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 851..868
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 864
FT /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT TURN 86..88
FT /evidence="ECO:0007829|PDB:3OVU"
FT HELIX 90..93
FT /evidence="ECO:0007829|PDB:3OVU"
FT HELIX 97..99
FT /evidence="ECO:0007829|PDB:3OVU"
FT STRAND 109..112
FT /evidence="ECO:0007829|PDB:3S48"
FT STRAND 114..117
FT /evidence="ECO:0007829|PDB:3OVU"
FT STRAND 121..123
FT /evidence="ECO:0007829|PDB:3S48"
FT HELIX 125..130
FT /evidence="ECO:0007829|PDB:3OVU"
FT STRAND 133..138
FT /evidence="ECO:0007829|PDB:3OVU"
FT STRAND 141..151
FT /evidence="ECO:0007829|PDB:3OVU"
FT TURN 153..155
FT /evidence="ECO:0007829|PDB:3OVU"
FT STRAND 156..165
FT /evidence="ECO:0007829|PDB:3OVU"
FT STRAND 166..168
FT /evidence="ECO:0007829|PDB:2H3K"
FT STRAND 171..176
FT /evidence="ECO:0007829|PDB:3OVU"
FT TURN 178..180
FT /evidence="ECO:0007829|PDB:3OVU"
FT STRAND 182..189
FT /evidence="ECO:0007829|PDB:3OVU"
FT STRAND 193..203
FT /evidence="ECO:0007829|PDB:3OVU"
FT STRAND 205..207
FT /evidence="ECO:0007829|PDB:3OVU"
FT STRAND 209..212
FT /evidence="ECO:0007829|PDB:3OVU"
FT STRAND 216..223
FT /evidence="ECO:0007829|PDB:3OVU"
SQ SEQUENCE 895 AA; 100847 MW; 797BB0D67F9A0559 CRC64;
MNKHHPKLRS FYSIRKSILG VASVIVSTLF LITSQHQAQA AENTNTSDKI SENQNNNATT
TQPPKDTNQT QPATQPANTA KTYPAADESL KDAIKDPALE NKEHDIGPRE QVNFQLLDKN
NETQYYHFFS IKDPADVYYT KKKAEVELDI NTASTWKKFE VYENNQKLPV RLVSYSPVPE
DHAYIRFPVS DGTQELKIVS STQIDDGEET NYDYTKLVFA KPIYNDPSLV KSDTNDAVVT
NDQSSSDASN QTNTNTSNQN TSTINNANNQ PQATTNMSQP AQPKSSANAD QASSQPAHET
NSNGNTNDKT NESSNQSDVN QQYPPADESL QDAIKNPAII DKEHTADNWR PIDFQMKNDK
GERQFYHYAS TVEPATVIFT KTGPIIELGL KTASTWKKFE VYEGDKKLPV ELVSYDSDKD
YAYIRFPVSN GTREVKIVSS IEYGENIHED YDYTLMVFAQ PITNNPDDYV DEETYNLQKL
LAPYHKAKTL ERQVYELEKL QEKLPEKYKA EYKKKLDQTR VELADQVKSA VTEFENVTPT
NDQLTDVQEA HFVVFESEEN SESVMDGFVE HPFYTATLNG QKYVVMKTKD DSYWKDLIVE
GKRVTTVSKD PKNNSRTLIF PYIPDKAVYN AIVKVVVANI GYEGQYHVRI INQDINTKDD
DTSQNNTSEP LNVQTGQEGK VADTDVAENS STATNPKDAS DKADVIEPDS DVVKDADNNI
DKDVQHDVDH LSDMSDNNHF DKYDLKEMDT QIAKDTDRNV DKGADNSVGM SSNVDTDKDS
NKNKDKVIQL NHIADKNNHN GKAAKLDVVK QNYNNTDKVT DKKTTEHLPS DIHKTVDKTV
KTKEKAGTPS KENKLSQSKM LPKTGETTSS QSWWGLYALL GMLALFIPKF RKESK
