ISF1_ARCFU
ID ISF1_ARCFU Reviewed; 220 AA.
AC O28836;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Iron-sulfur flavoprotein AF_1436;
DE Short=AF-1;
DE Short=Af1;
DE Short=Isf-1;
GN OrderedLocusNames=AF_1436;
OS Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS 100126 / VC-16).
OC Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC Archaeoglobus.
OX NCBI_TaxID=224325;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA Smith H.O., Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
RN [2]
RP PROTEIN FAMILY.
RX PubMed=10986231; DOI=10.1128/jb.182.19.5309-5316.2000;
RA Leartsakulpanich U., Antonkine M.L., Ferry J.G.;
RT "Site-specific mutational analysis of a novel cysteine motif proposed to
RT ligate the 4Fe-4S cluster in the iron-sulfur flavoprotein of the
RT thermophilic methanoarchaeon Methanosarcina thermophila.";
RL J. Bacteriol. 182:5309-5316(2000).
CC -!- FUNCTION: Redox-active protein probably involved in electron transport.
CC {ECO:0000250}.
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC Note=Binds 1 FMN per subunit. {ECO:0000250};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SsuE family. Isf subfamily. {ECO:0000305}.
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DR EMBL; AE000782; AAB89809.1; -; Genomic_DNA.
DR PIR; C69429; C69429.
DR RefSeq; WP_010878933.1; NC_000917.1.
DR AlphaFoldDB; O28836; -.
DR SMR; O28836; -.
DR STRING; 224325.AF_1436; -.
DR EnsemblBacteria; AAB89809; AAB89809; AF_1436.
DR GeneID; 24795048; -.
DR KEGG; afu:AF_1436; -.
DR eggNOG; arCOG02572; Archaea.
DR HOGENOM; CLU_050993_1_0_2; -.
DR OMA; TIMERMC; -.
DR OrthoDB; 89591at2157; -.
DR PhylomeDB; O28836; -.
DR Proteomes; UP000002199; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR Gene3D; 3.40.50.360; -; 1.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR005025; FMN_Rdtase-like.
DR Pfam; PF03358; FMN_red; 1.
DR SUPFAM; SSF52218; SSF52218; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Flavoprotein; FMN; Iron; Iron-sulfur; Metal-binding;
KW Reference proteome.
FT CHAIN 1..220
FT /note="Iron-sulfur flavoprotein AF_1436"
FT /id="PRO_0000332942"
FT BINDING 47
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 50
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 53
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 59
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
SQ SEQUENCE 220 AA; 24361 MW; 9D89441DCEC6D35C CRC64;
MKAVGILGSP RKYGNASKML DAALKELENS GFEVEKVHIS SKKINYCTGC GTCLAKGECV
QRDDMDELKR LVEESDAVIL ASPVYYLNVT AQMKTFIDRM LPYGHRPTLK GKYGGSIVVY
AGVGKPEEVA GYMNRVLKAW GIVPVGYAVG FGVIPGEVGD EDLKKASQLG SKIAEAFESK
YRMEPSDEDL ELQKQLLTLI KNYGHLMKAD YEFWKEKGFI
