ID   ISF1_METJA              Reviewed;         198 AA.
AC   Q58141;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Iron-sulfur flavoprotein MJ0731;
DE            Short=Isf-1;
DE   AltName: Full=MCJ-1;
DE            Short=Mj1;
GN   OrderedLocusNames=MJ0731;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   PROTEIN FAMILY.
RX   PubMed=10986231; DOI=10.1128/jb.182.19.5309-5316.2000;
RA   Leartsakulpanich U., Antonkine M.L., Ferry J.G.;
RT   "Site-specific mutational analysis of a novel cysteine motif proposed to
RT   ligate the 4Fe-4S cluster in the iron-sulfur flavoprotein of the
RT   thermophilic methanoarchaeon Methanosarcina thermophila.";
RL   J. Bacteriol. 182:5309-5316(2000).
CC   -!- FUNCTION: Redox-active protein probably involved in electron transport.
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC       Note=Binds 1 FMN per subunit. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the SsuE family. Isf subfamily. {ECO:0000305}.
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DR   EMBL; L77117; AAB98727.1; -; Genomic_DNA.
DR   PIR; C64391; C64391.
DR   RefSeq; WP_010870236.1; NC_000909.1.
DR   AlphaFoldDB; Q58141; -.
DR   SMR; Q58141; -.
DR   STRING; 243232.MJ_0731; -.
DR   EnsemblBacteria; AAB98727; AAB98727; MJ_0731.
DR   GeneID; 1451608; -.
DR   KEGG; mja:MJ_0731; -.
DR   eggNOG; arCOG02573; Archaea.
DR   HOGENOM; CLU_050993_3_0_2; -.
DR   InParanoid; Q58141; -.
DR   OMA; NLGATFW; -.
DR   OrthoDB; 89591at2157; -.
DR   PhylomeDB; Q58141; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   Gene3D; 3.40.50.360; -; 1.
DR   InterPro; IPR029039; Flavoprotein-like_sf.
DR   InterPro; IPR005025; FMN_Rdtase-like.
DR   Pfam; PF03358; FMN_red; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Flavoprotein; FMN; Iron; Iron-sulfur; Metal-binding;
KW   Reference proteome.
FT   CHAIN           1..198
FT                   /note="Iron-sulfur flavoprotein MJ0731"
FT                   /id="PRO_0000160599"
FT   BINDING         46
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         49
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         52
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         59
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   198 AA;  22220 MW;  FBED0E57A4C77017 CRC64;
     MKVFGISGSP RLQGTHFAVN YALNYLKEKG AEVRYFSVSR KKINFCLHCD YCIKKKEGCI
     HKDDMEEVYE NLIWADGVII GTPVYQGNVT GQLKTLMDRC RAILAKNPKV LRGRVGMAIA
     VGGDRNGGQE IALRTIHDFF IINEMIPVGG GSFGANLGAT FWSKDRGKKG VEEDEEGLRV
     LRKTLNRFYE VLKEKRGL