APTF2_CAEEL
ID APTF2_CAEEL Reviewed; 367 AA.
AC G2HK15; Q9U1W4;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Transcription factor aptf-2 {ECO:0000303|PubMed:27176626};
GN Name=aptf-2 {ECO:0000312|WormBase:Y62E10A.17b};
GN ORFNames=Y62E10A.17 {ECO:0000312|WormBase:Y62E10A.17b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLU-182.
RX PubMed=27176626; DOI=10.1371/journal.pgen.1006048;
RA Budirahardja Y., Tan P.Y., Doan T., Weisdepp P., Zaidel-Bar R.;
RT "The AP-2 Transcription Factor APTF-2 Is Required for Neuroblast and
RT Epidermal Morphogenesis in Caenorhabditis elegans Embryogenesis.";
RL PLoS Genet. 12:e1006048-e1006048(2016).
CC -!- FUNCTION: Sequence-specific DNA-binding protein that interacts with
CC enhancer elements to regulate transcription of selected genes (By
CC similarity). Required for neuroblast and epidermal morphogenesis,
CC perhaps acting in cooperation with transcription factor aptf-4
CC (PubMed:27176626). {ECO:0000250|UniProtKB:P05549,
CC ECO:0000269|PubMed:27176626}.
CC -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000250|UniProtKB:P05549}.
CC -!- INTERACTION:
CC G2HK15; Q19862: aptf-3; NbExp=3; IntAct=EBI-2416772, EBI-327600;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:27176626}. Cytoplasm
CC {ECO:0000269|PubMed:27176626}. Note=Localized to both cytoplasm and
CC nucleus in most cells, but significant nuclear enrichment in
CC neuroblasts and epidermal cells in AB lineage during ventral cleft
CC closure and in epidermal cells in C lineage preceding dorsal
CC intercalation. {ECO:0000269|PubMed:27176626}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b {ECO:0000312|WormBase:Y62E10A.17b};
CC IsoId=G2HK15-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:Y62E10A.17a};
CC IsoId=G2HK15-2; Sequence=VSP_061131;
CC -!- SIMILARITY: Belongs to the AP-2 family. {ECO:0000303|PubMed:27176626}.
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DR EMBL; BX284604; CAB60610.1; -; Genomic_DNA.
DR EMBL; BX284604; CCD31155.1; -; Genomic_DNA.
DR RefSeq; NP_001255740.1; NM_001268811.1.
DR AlphaFoldDB; G2HK15; -.
DR IntAct; G2HK15; 27.
DR STRING; 6239.Y62E10A.17b; -.
DR PaxDb; G2HK15; -.
DR EnsemblMetazoa; Y62E10A.17a.1; Y62E10A.17a.1; WBGene00013383. [G2HK15-2]
DR EnsemblMetazoa; Y62E10A.17b.1; Y62E10A.17b.1; WBGene00013383. [G2HK15-1]
DR GeneID; 190458; -.
DR KEGG; cel:CELE_Y62E10A.17; -.
DR UCSC; Y62E10A.17; c. elegans.
DR CTD; 190458; -.
DR WormBase; Y62E10A.17a; CE25526; WBGene00013383; aptf-2.
DR WormBase; Y62E10A.17b; CE46244; WBGene00013383; aptf-2.
DR eggNOG; KOG3811; Eukaryota.
DR GeneTree; ENSGT00950000182848; -.
DR HOGENOM; CLU_754852_0_0_1; -.
DR InParanoid; G2HK15; -.
DR OMA; DICIDEI; -.
DR OrthoDB; 1575964at2759; -.
DR PhylomeDB; G2HK15; -.
DR Reactome; R-CEL-3232118; SUMOylation of transcription factors.
DR Reactome; R-CEL-8866904; Negative regulation of activity of TFAP2 (AP-2) family transcription factors.
DR Reactome; R-CEL-8866907; Activation of the TFAP2 (AP-2) family of transcription factors.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00013383; Expressed in embryo and 4 other tissues.
DR ExpressionAtlas; G2HK15; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0042127; P:regulation of cell population proliferation; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR004979; TF_AP2.
DR InterPro; IPR013854; TF_AP2_C.
DR PANTHER; PTHR10812; PTHR10812; 1.
DR Pfam; PF03299; TF_AP-2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; DNA-binding;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..367
FT /note="Transcription factor aptf-2"
FT /id="PRO_0000453375"
FT REGION 29..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 220..354
FT /note="H-S-H (helix-span-helix), dimerization"
FT /evidence="ECO:0000250|UniProtKB:P05549"
FT COMPBIAS 31..49
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..4
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_061131"
FT MUTAGEN 182
FT /note="E->K: In qm27; embryonic lethality and morphological
FT defects in larva. Embryos show failure in dorsal epidermal
FT cell intercalation, failure of ventral epidermal cell
FT enclosure, and arrest during elongation. Causes aberrant
FT nuclear localization in neuroblasts. Nuclear expression of
FT zinc-finger protein die-1 is significantly reduced.
FT Embryonic lethality exacerbated by simultaneous RNAi-
FT mediated knockdown of transcription factor aptf-4."
FT /evidence="ECO:0000269|PubMed:27176626"
SQ SEQUENCE 367 AA; 39931 MW; 47BFEA08975B2C86 CRC64;
MDIQMFTTLN STVPTEPVVG PVEPTVPTVP ATKETGPSSS AECSTQPAVA EQEDNSLLAQ
LLKGKITSDD AKLQPPRGPF EYAPMLSEEA QFAIKLAGLL NPNPVTEQDP STFKPPPKMS
NNVRKNINLT PVIENAVPSG FELESPKDQA FDVVHGRLSM GQNQTCYFVT VEEMRRRLQS
PEKLNSSSIA CNLKRPKLKN GGELMRAQLR DHGIKVQLNA KQKAFPNKVI ALVEAEAVHL
GLDLGTAVRD DYPVQDIIQE VAHEALADED ADLDALMNDK DFTECMSALE SVFTSVVPPI
TGIEPKPSNN MRLNNGMETF SSASHGLGIV SQRVWLPQLT AIGNGVASEL RRLTETPTES
KPEELKA
