ISF2_ARCFU
ID ISF2_ARCFU Reviewed; 195 AA.
AC O28753;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Iron-sulfur flavoprotein AF_1519;
DE AltName: Full=AF-2;
DE Short=Af2;
DE AltName: Full=Isf-2;
GN OrderedLocusNames=AF_1519;
OS Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS 100126 / VC-16).
OC Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC Archaeoglobus.
OX NCBI_TaxID=224325;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA Smith H.O., Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
RN [2]
RP PROTEIN FAMILY.
RX PubMed=10986231; DOI=10.1128/jb.182.19.5309-5316.2000;
RA Leartsakulpanich U., Antonkine M.L., Ferry J.G.;
RT "Site-specific mutational analysis of a novel cysteine motif proposed to
RT ligate the 4Fe-4S cluster in the iron-sulfur flavoprotein of the
RT thermophilic methanoarchaeon Methanosarcina thermophila.";
RL J. Bacteriol. 182:5309-5316(2000).
CC -!- FUNCTION: Redox-active protein probably involved in electron transport.
CC {ECO:0000250}.
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC Note=Binds 1 FMN per subunit. {ECO:0000250};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SsuE family. Isf subfamily. {ECO:0000305}.
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DR EMBL; AE000782; AAB89727.1; -; Genomic_DNA.
DR PIR; F69439; F69439.
DR RefSeq; WP_010879016.1; NC_000917.1.
DR AlphaFoldDB; O28753; -.
DR SMR; O28753; -.
DR STRING; 224325.AF_1519; -.
DR DNASU; 1484747; -.
DR EnsemblBacteria; AAB89727; AAB89727; AF_1519.
DR GeneID; 24795267; -.
DR KEGG; afu:AF_1519; -.
DR eggNOG; arCOG02573; Archaea.
DR HOGENOM; CLU_050993_3_0_2; -.
DR OMA; NLGATFW; -.
DR OrthoDB; 89591at2157; -.
DR PhylomeDB; O28753; -.
DR Proteomes; UP000002199; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR Gene3D; 3.40.50.360; -; 1.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR005025; FMN_Rdtase-like.
DR Pfam; PF03358; FMN_red; 1.
DR SUPFAM; SSF52218; SSF52218; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Flavoprotein; FMN; Iron; Iron-sulfur; Metal-binding;
KW Reference proteome.
FT CHAIN 1..195
FT /note="Iron-sulfur flavoprotein AF_1519"
FT /id="PRO_0000332943"
FT BINDING 45
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 48
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 51
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 57
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
SQ SEQUENCE 195 AA; 21682 MW; 5DBDE9BCE14E932B CRC64;
MIVGISGSPR RKATEFVLGE ALKMLEERGF ETKFFTVRGK KISPCQHCDY CLKHKECRIK
DDMFELYEML KDAKGIVMAT PVYNGGVSAQ IKAVMDRCRA LVAADYDFFR GKVGMAIAVG
GDRIGGQELA IQQILTFYIL NGVIPVSGGS FGANIGATFW SRDTLEGVKE DEEGFRSLRK
TVKRFAEMLE KMEGV
