ID   ISF2_METJA              Reviewed;         193 AA.
AC   Q58483;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Iron-sulfur flavoprotein MJ1083;
DE   AltName: Full=Isf-2;
DE   AltName: Full=MCJ-2;
DE            Short=Mj2;
GN   OrderedLocusNames=MJ1083;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   PROTEIN SEQUENCE OF N-TERMINUS, COFACTOR, AND IRON-SULFUR CLUSTER.
RX   PubMed=11591665; DOI=10.1128/jb.183.21.6225-6233.2001;
RA   Zhao T., Cruz F., Ferry J.G.;
RT   "Iron-sulfur flavoprotein (Isf) from Methanosarcina thermophila is the
RT   prototype of a widely distributed family.";
RL   J. Bacteriol. 183:6225-6233(2001).
RN   [3]
RP   PROTEIN FAMILY.
RX   PubMed=10986231; DOI=10.1128/jb.182.19.5309-5316.2000;
RA   Leartsakulpanich U., Antonkine M.L., Ferry J.G.;
RT   "Site-specific mutational analysis of a novel cysteine motif proposed to
RT   ligate the 4Fe-4S cluster in the iron-sulfur flavoprotein of the
RT   thermophilic methanoarchaeon Methanosarcina thermophila.";
RL   J. Bacteriol. 182:5309-5316(2000).
CC   -!- FUNCTION: Redox-active protein probably involved in electron transport.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000269|PubMed:11591665};
CC       Note=Binds 1 FMN per subunit. {ECO:0000269|PubMed:11591665};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000305|PubMed:11591665};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit.
CC       {ECO:0000305|PubMed:11591665};
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the SsuE family. Isf subfamily. {ECO:0000305}.
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DR   EMBL; L77117; AAB99085.1; -; Genomic_DNA.
DR   PIR; B64435; B64435.
DR   RefSeq; WP_010870595.1; NC_000909.1.
DR   AlphaFoldDB; Q58483; -.
DR   SMR; Q58483; -.
DR   STRING; 243232.MJ_1083; -.
DR   EnsemblBacteria; AAB99085; AAB99085; MJ_1083.
DR   GeneID; 1451979; -.
DR   KEGG; mja:MJ_1083; -.
DR   eggNOG; arCOG02573; Archaea.
DR   HOGENOM; CLU_050993_3_0_2; -.
DR   InParanoid; Q58483; -.
DR   OMA; KFADGNP; -.
DR   OrthoDB; 89591at2157; -.
DR   PhylomeDB; Q58483; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   Gene3D; 3.40.50.360; -; 1.
DR   InterPro; IPR029039; Flavoprotein-like_sf.
DR   InterPro; IPR005025; FMN_Rdtase-like.
DR   Pfam; PF03358; FMN_red; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
PE   1: Evidence at protein level;
KW   4Fe-4S; Direct protein sequencing; Flavoprotein; FMN; Iron; Iron-sulfur;
KW   Metal-binding; Reference proteome.
FT   CHAIN           1..193
FT                   /note="Iron-sulfur flavoprotein MJ1083"
FT                   /id="PRO_0000160600"
FT   BINDING         47
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         50
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         53
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         59
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   193 AA;  20648 MW;  EB58694A037D2F0B CRC64;
     MKVIGISGSP RPEGNTTLLV REALNAIAEE GIETEFISLA DKELNPCIGC NMCKEEGKCP
     IIDDVDEILK KMKEADGIIL GSPVYFGGVS AQLKMLMDRS RPLRIGFQLR NKVGGAVAVG
     ASRNGGQETT IQQIHNFFLI HSMIVVGDND PTAHYGGTGV GKAPGDCKND DIGLETARNL
     GKKVAEVVKL IKK