ISF3_ARCFU
ID ISF3_ARCFU Reviewed; 201 AA.
AC O28383;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Iron-sulfur flavoprotein AF_1896;
DE AltName: Full=AF-3;
DE Short=Af3;
DE AltName: Full=Isf-3;
GN OrderedLocusNames=AF_1896;
OS Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS 100126 / VC-16).
OC Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC Archaeoglobus.
OX NCBI_TaxID=224325;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA Smith H.O., Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
RN [2]
RP PROTEIN SEQUENCE OF N-TERMINUS, COFACTOR, AND IRON-SULFUR CLUSTER.
RX PubMed=11591665; DOI=10.1128/jb.183.21.6225-6233.2001;
RA Zhao T., Cruz F., Ferry J.G.;
RT "Iron-sulfur flavoprotein (Isf) from Methanosarcina thermophila is the
RT prototype of a widely distributed family.";
RL J. Bacteriol. 183:6225-6233(2001).
RN [3]
RP PROTEIN FAMILY.
RX PubMed=10986231; DOI=10.1128/jb.182.19.5309-5316.2000;
RA Leartsakulpanich U., Antonkine M.L., Ferry J.G.;
RT "Site-specific mutational analysis of a novel cysteine motif proposed to
RT ligate the 4Fe-4S cluster in the iron-sulfur flavoprotein of the
RT thermophilic methanoarchaeon Methanosarcina thermophila.";
RL J. Bacteriol. 182:5309-5316(2000).
CC -!- FUNCTION: Redox-active protein probably involved in electron transport.
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000269|PubMed:11591665};
CC Note=Binds 1 FMN per subunit. {ECO:0000269|PubMed:11591665};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000305|PubMed:11591665};
CC Note=Binds 1 [4Fe-4S] cluster per subunit.
CC {ECO:0000305|PubMed:11591665};
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the SsuE family. Isf subfamily. {ECO:0000305}.
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DR EMBL; AE000782; AAB89371.1; -; Genomic_DNA.
DR PIR; G69486; G69486.
DR RefSeq; WP_010879389.1; NC_000917.1.
DR AlphaFoldDB; O28383; -.
DR SMR; O28383; -.
DR STRING; 224325.AF_1896; -.
DR DNASU; 1485119; -.
DR EnsemblBacteria; AAB89371; AAB89371; AF_1896.
DR GeneID; 24795640; -.
DR KEGG; afu:AF_1896; -.
DR eggNOG; arCOG02573; Archaea.
DR HOGENOM; CLU_050993_1_0_2; -.
DR OMA; MHIHGMI; -.
DR OrthoDB; 89591at2157; -.
DR PhylomeDB; O28383; -.
DR Proteomes; UP000002199; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR Gene3D; 3.40.50.360; -; 1.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR005025; FMN_Rdtase-like.
DR Pfam; PF03358; FMN_red; 1.
DR SUPFAM; SSF52218; SSF52218; 1.
PE 1: Evidence at protein level;
KW 4Fe-4S; Direct protein sequencing; Flavoprotein; FMN; Iron; Iron-sulfur;
KW Metal-binding; Reference proteome.
FT CHAIN 1..201
FT /note="Iron-sulfur flavoprotein AF_1896"
FT /id="PRO_0000332944"
FT BINDING 46
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 49
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 52
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 57
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
SQ SEQUENCE 201 AA; 22027 MW; E678BB64C7FD9B5D CRC64;
MKLLAINGSP NKRNTLFLLE VIAEEVKKLG HEAEIIHLKD YEIKECKGCD ACLKGDCSQK
DDIYKVLEKM QEADAIVIGT PTYFGNVTGI VKNLIDRSRM ARMGNYRLRN RVFAPVVTSG
LRNGGAEYAA MSLIVYALGQ AMLPVSIVEN PITTGTFPVG VIQGDAGWRS VKKDEIAINS
AKALAKRIVE VAEATKNLRE S
