ID   ISF_CLOD6               Reviewed;         225 AA.
AC   Q188R7;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Iron-sulfur flavoprotein CD630_04720;
DE            Short=Isf;
DE   AltName: Full=Cd1;
DE            Short=CD;
GN   OrderedLocusNames=CD630_04720;
OS   Clostridioides difficile (strain 630) (Peptoclostridium difficile).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC   Clostridioides.
OX   NCBI_TaxID=272563;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=630;
RX   PubMed=16804543; DOI=10.1038/ng1830;
RA   Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N.,
RA   Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H.,
RA   Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N.,
RA   Brooks K., Chillingworth T., Cronin A., Davis P., Dowd L., Fraser A.,
RA   Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K.,
RA   Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L.,
RA   Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J.;
RT   "The multidrug-resistant human pathogen Clostridium difficile has a highly
RT   mobile, mosaic genome.";
RL   Nat. Genet. 38:779-786(2006).
RN   [2]
RP   PROTEIN SEQUENCE OF N-TERMINUS, COFACTOR, AND IRON-SULFUR CLUSTER.
RX   PubMed=11591665; DOI=10.1128/jb.183.21.6225-6233.2001;
RA   Zhao T., Cruz F., Ferry J.G.;
RT   "Iron-sulfur flavoprotein (Isf) from Methanosarcina thermophila is the
RT   prototype of a widely distributed family.";
RL   J. Bacteriol. 183:6225-6233(2001).
RN   [3]
RP   PROTEIN FAMILY.
RX   PubMed=10986231; DOI=10.1128/jb.182.19.5309-5316.2000;
RA   Leartsakulpanich U., Antonkine M.L., Ferry J.G.;
RT   "Site-specific mutational analysis of a novel cysteine motif proposed to
RT   ligate the 4Fe-4S cluster in the iron-sulfur flavoprotein of the
RT   thermophilic methanoarchaeon Methanosarcina thermophila.";
RL   J. Bacteriol. 182:5309-5316(2000).
CC   -!- FUNCTION: Redox-active protein probably involved in electron transport.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000269|PubMed:11591665};
CC       Note=Binds 1 FMN per subunit. {ECO:0000269|PubMed:11591665};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000305|PubMed:11591665};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit.
CC       {ECO:0000305|PubMed:11591665};
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the SsuE family. Isf subfamily. {ECO:0000305}.
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DR   EMBL; AM180355; CAJ67300.1; -; Genomic_DNA.
DR   RefSeq; WP_003434723.1; NZ_CP010905.2.
DR   RefSeq; YP_001086945.1; NC_009089.1.
DR   AlphaFoldDB; Q188R7; -.
DR   SMR; Q188R7; -.
DR   STRING; 272563.CD630_04720; -.
DR   DNASU; 4914243; -.
DR   EnsemblBacteria; CAJ67300; CAJ67300; CD630_04720.
DR   GeneID; 66352974; -.
DR   KEGG; cdf:CD630_04720; -.
DR   KEGG; pdc:CDIF630_00600; -.
DR   PATRIC; fig|272563.120.peg.497; -.
DR   eggNOG; COG0655; Bacteria.
DR   OMA; GQMKVFF; -.
DR   PhylomeDB; Q188R7; -.
DR   BioCyc; PDIF272563:G12WB-584-MON; -.
DR   Proteomes; UP000001978; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   Gene3D; 3.40.50.360; -; 1.
DR   InterPro; IPR029039; Flavoprotein-like_sf.
DR   InterPro; IPR005025; FMN_Rdtase-like.
DR   Pfam; PF03358; FMN_red; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
PE   1: Evidence at protein level;
KW   4Fe-4S; Direct protein sequencing; Flavoprotein; FMN; Iron; Iron-sulfur;
KW   Metal-binding; Reference proteome.
FT   CHAIN           1..225
FT                   /note="Iron-sulfur flavoprotein CD630_04720"
FT                   /id="PRO_0000332941"
FT   BINDING         49
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         52
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         55
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         61
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   225 AA;  25445 MW;  03A4E38C84D6B739 CRC64;
     MIITVINGSP RKNGATSKVL TYLYKDIERL IPDVKINYFD LSEVNPSYCI GCLNCYKMGK
     CINQNDKVEY IHDIITKSDG VIFGSPTYGS SVTGLFKVFT DRAHMMLERL LYRKPCIAVT
     TYENARGSKA ISFIKSMVLD SGGYVCGSLS IKTGFNQNPI TEKVESKIQK VSKKFIYCIE
     EKKNPPVLSQ IYNFIAINAV LKPMAFKDIE QYKGIIDRWE EQGII