ISK10_MOUSE
ID ISK10_MOUSE Reviewed; 162 AA.
AC Q8CAC8;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Serine protease inhibitor Kazal-type 10;
GN Name=Spink10;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=FVB/N; TISSUE=Epididymis;
RX PubMed=16930550; DOI=10.1016/j.bbrc.2006.08.023;
RA Jalkanen J., Kotimaeki M., Huhtaniemi I., Poutanen M.;
RT "Novel epididymal protease inhibitors with Kazal or WAP family domain.";
RL Biochem. Biophys. Res. Commun. 349:245-254(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Hypothalamus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probable serine protease inhibitor.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in epydiymis, in the cauda and corpus.
CC Also expressed in brain. {ECO:0000269|PubMed:16930550}.
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DR EMBL; DQ437330; ABD93325.1; -; mRNA.
DR EMBL; AK039062; BAC30226.1; -; mRNA.
DR EMBL; BC125360; AAI25361.1; -; mRNA.
DR EMBL; BC125362; AAI25363.1; -; mRNA.
DR CCDS; CCDS29293.1; -.
DR RefSeq; NP_808497.1; NM_177829.3.
DR RefSeq; XP_006526094.1; XM_006526031.3.
DR RefSeq; XP_006526096.1; XM_006526033.3.
DR RefSeq; XP_006526097.1; XM_006526034.3.
DR RefSeq; XP_006526098.1; XM_006526035.3.
DR RefSeq; XP_011245251.1; XM_011246949.2.
DR AlphaFoldDB; Q8CAC8; -.
DR BioGRID; 236685; 1.
DR STRING; 10090.ENSMUSP00000062976; -.
DR MEROPS; I01.979; -.
DR iPTMnet; Q8CAC8; -.
DR PhosphoSitePlus; Q8CAC8; -.
DR PaxDb; Q8CAC8; -.
DR PRIDE; Q8CAC8; -.
DR ProteomicsDB; 267010; -.
DR DNASU; 328971; -.
DR Ensembl; ENSMUST00000055725; ENSMUSP00000062976; ENSMUSG00000044176.
DR GeneID; 328971; -.
DR KEGG; mmu:328971; -.
DR UCSC; uc008fdf.1; mouse.
DR CTD; 328971; -.
DR MGI; MGI:3584533; Spink10.
DR VEuPathDB; HostDB:ENSMUSG00000044176; -.
DR eggNOG; ENOG502SEPY; Eukaryota.
DR GeneTree; ENSGT00940000164814; -.
DR HOGENOM; CLU_087965_2_0_1; -.
DR InParanoid; Q8CAC8; -.
DR OMA; YSETAFT; -.
DR PhylomeDB; Q8CAC8; -.
DR BioGRID-ORCS; 328971; 4 hits in 71 CRISPR screens.
DR ChiTaRS; Spink10; mouse.
DR PRO; PR:Q8CAC8; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q8CAC8; protein.
DR Bgee; ENSMUSG00000044176; Expressed in cortical plate and 60 other tissues.
DR ExpressionAtlas; Q8CAC8; baseline and differential.
DR Genevisible; Q8CAC8; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR InterPro; IPR001239; Prot_inh_Kazal-m.
DR Pfam; PF00050; Kazal_1; 2.
DR PRINTS; PR00290; KAZALINHBTR.
DR SMART; SM00280; KAZAL; 2.
DR SUPFAM; SSF100895; SSF100895; 2.
DR PROSITE; PS00282; KAZAL_1; 2.
DR PROSITE; PS51465; KAZAL_2; 2.
PE 2: Evidence at transcript level;
KW Disulfide bond; Membrane; Protease inhibitor; Reference proteome; Repeat;
KW Serine protease inhibitor; Transmembrane; Transmembrane helix.
FT CHAIN 1..162
FT /note="Serine protease inhibitor Kazal-type 10"
FT /id="PRO_0000302141"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 29..86
FT /note="Kazal-like 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DOMAIN 106..162
FT /note="Kazal-like 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT SITE 124..125
FT /note="Reactive bond"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 35..67
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 45..64
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 112..144
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 122..141
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 130..162
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
SQ SEQUENCE 162 AA; 18520 MW; C4733FE2836E21A8 CRC64;
MKLSSLWSNA FVINVAIALY AETAFLSAPK LKIDCRPYLD SNDKCTREYH PVCSTSGKTY
CNKCTFCKAL RLDTMSSSLL WIKITFILAL VVPFYYGTTF AFSKEARRQP DCDKYRTFPN
QCTREWNPVC GTNGFTYSNE CVFCNAKIAA KEKIDYRHFG PC
