ISK4_HUMAN
ID ISK4_HUMAN Reviewed; 86 AA.
AC O60575; Q2YDT7;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Serine protease inhibitor Kazal-type 4;
DE AltName: Full=Peptide PEC-60 homolog;
DE Flags: Precursor;
GN Name=SPINK4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Hu G.;
RT "Human gastrointestinal peptide mRNA, homolog of swine PEC-60.";
RL Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- INTERACTION:
CC O60575; Q96EQ0: SGTB; NbExp=3; IntAct=EBI-13336697, EBI-744081;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
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DR EMBL; AF048700; AAC05124.1; -; mRNA.
DR EMBL; AL161445; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC110068; AAI10069.1; -; mRNA.
DR CCDS; CCDS6536.1; -.
DR RefSeq; NP_055286.1; NM_014471.2.
DR AlphaFoldDB; O60575; -.
DR SMR; O60575; -.
DR BioGRID; 118114; 21.
DR IntAct; O60575; 9.
DR STRING; 9606.ENSP00000369045; -.
DR MEROPS; I01.970; -.
DR BioMuta; SPINK4; -.
DR jPOST; O60575; -.
DR MassIVE; O60575; -.
DR PaxDb; O60575; -.
DR PeptideAtlas; O60575; -.
DR PRIDE; O60575; -.
DR ProteomicsDB; 49476; -.
DR Antibodypedia; 1725; 62 antibodies from 12 providers.
DR DNASU; 27290; -.
DR Ensembl; ENST00000379721.4; ENSP00000369045.3; ENSG00000122711.9.
DR GeneID; 27290; -.
DR KEGG; hsa:27290; -.
DR MANE-Select; ENST00000379721.4; ENSP00000369045.3; NM_014471.3; NP_055286.1.
DR CTD; 27290; -.
DR DisGeNET; 27290; -.
DR GeneCards; SPINK4; -.
DR HGNC; HGNC:16646; SPINK4.
DR HPA; ENSG00000122711; Tissue enriched (intestine).
DR MIM; 613929; gene.
DR neXtProt; NX_O60575; -.
DR OpenTargets; ENSG00000122711; -.
DR PharmGKB; PA38180; -.
DR VEuPathDB; HostDB:ENSG00000122711; -.
DR eggNOG; KOG3649; Eukaryota.
DR GeneTree; ENSGT00530000064269; -.
DR HOGENOM; CLU_169765_2_0_1; -.
DR InParanoid; O60575; -.
DR OMA; HQWVVTL; -.
DR OrthoDB; 1588023at2759; -.
DR PhylomeDB; O60575; -.
DR PathwayCommons; O60575; -.
DR SignaLink; O60575; -.
DR BioGRID-ORCS; 27290; 18 hits in 1076 CRISPR screens.
DR ChiTaRS; SPINK4; human.
DR GenomeRNAi; 27290; -.
DR Pharos; O60575; Tdark.
DR PRO; PR:O60575; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; O60575; protein.
DR Bgee; ENSG00000122711; Expressed in ileal mucosa and 116 other tissues.
DR ExpressionAtlas; O60575; baseline and differential.
DR Genevisible; O60575; HS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR InterPro; IPR039932; Spink4-like.
DR PANTHER; PTHR21179; PTHR21179; 1.
DR Pfam; PF00050; Kazal_1; 1.
DR SMART; SM00280; KAZAL; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR PROSITE; PS00282; KAZAL_1; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Protease inhibitor; Reference proteome; Secreted;
KW Serine protease inhibitor; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000250"
FT CHAIN 27..86
FT /note="Serine protease inhibitor Kazal-type 4"
FT /id="PRO_0000016569"
FT DOMAIN 31..86
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT SITE 48..49
FT /note="Reactive bond"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 37..68
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 46..65
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 54..86
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT VARIANT 7
FT /note="V -> I (in dbSNP:rs706107)"
FT /id="VAR_011898"
SQ SEQUENCE 86 AA; 9454 MW; 374CD4B3CAC9AF52 CRC64;
MAVRQWVIAL ALAALLVVDR EVPVAAGKLP FSRMPICEHM VESPTCSQMS NLVCGTDGLT
YTNECQLCLA RIKTKQDIQI MKDGKC
