ISK4_MOUSE
ID ISK4_MOUSE Reviewed; 86 AA.
AC O35679; Q149Z4;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Serine protease inhibitor Kazal-type 4;
DE AltName: Full=MPGC60 protein;
DE AltName: Full=Peptide PEC-60 homolog;
DE Flags: Precursor;
GN Name=Spink4; Synonyms=Mpgc60;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=NMRI; TISSUE=Intestine;
RX PubMed=9810707; DOI=10.1046/j.1432-0436.1998.6350285.x;
RA Krause R., Hemberger M., Messerschmid M., Mayer W., Kothary R., Dixkens C.,
RA Fundele R.;
RT "Molecular cloning and characterization of murine Mpgc60, a gene
RT predominantly expressed in the intestinal tract.";
RL Differentiation 63:285-294(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in the intestinal tract.
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DR EMBL; Y11505; CAA72282.1; -; mRNA.
DR EMBL; AL837521; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466538; EDL05424.1; -; Genomic_DNA.
DR EMBL; BC117048; AAI17049.1; -; mRNA.
DR EMBL; BC117050; AAI17051.1; -; mRNA.
DR CCDS; CCDS18052.1; -.
DR RefSeq; NP_035593.2; NM_011463.2.
DR AlphaFoldDB; O35679; -.
DR SMR; O35679; -.
DR STRING; 10090.ENSMUSP00000030122; -.
DR MEROPS; I01.970; -.
DR PaxDb; O35679; -.
DR PRIDE; O35679; -.
DR Antibodypedia; 1725; 62 antibodies from 12 providers.
DR DNASU; 20731; -.
DR Ensembl; ENSMUST00000030122; ENSMUSP00000030122; ENSMUSG00000028415.
DR GeneID; 20731; -.
DR KEGG; mmu:20731; -.
DR UCSC; uc008shy.1; mouse.
DR CTD; 27290; -.
DR MGI; MGI:1341848; Spink4.
DR VEuPathDB; HostDB:ENSMUSG00000028415; -.
DR eggNOG; KOG3649; Eukaryota.
DR GeneTree; ENSGT00530000064269; -.
DR HOGENOM; CLU_169765_2_0_1; -.
DR InParanoid; O35679; -.
DR OMA; HQWVVTL; -.
DR OrthoDB; 1588023at2759; -.
DR PhylomeDB; O35679; -.
DR BioGRID-ORCS; 20731; 1 hit in 73 CRISPR screens.
DR PRO; PR:O35679; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; O35679; protein.
DR Bgee; ENSMUSG00000028415; Expressed in crypt of Lieberkuhn of small intestine and 91 other tissues.
DR Genevisible; O35679; MM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR InterPro; IPR039932; Spink4-like.
DR PANTHER; PTHR21179; PTHR21179; 1.
DR Pfam; PF00050; Kazal_1; 1.
DR SMART; SM00280; KAZAL; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR PROSITE; PS00282; KAZAL_1; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Protease inhibitor; Reference proteome; Secreted;
KW Serine protease inhibitor; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000250"
FT CHAIN 27..86
FT /note="Serine protease inhibitor Kazal-type 4"
FT /id="PRO_0000016570"
FT DOMAIN 31..86
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT SITE 48..49
FT /note="Reactive bond"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 37..68
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 46..65
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 54..86
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT CONFLICT 13
FT /note="V -> G (in Ref. 1; CAA72282)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 86 AA; 9729 MW; BC6D5E6204A597A5 CRC64;
MAMHLWLVTL TLVPLLGMDR ELMVSAGSLV FPRMPFCEHM AELPNCPQTP NLICGTDGLT
YENECHLCLT RMKTMKDIQI MKDGQC
