ISK7_HUMAN
ID ISK7_HUMAN Reviewed; 85 AA.
AC P58062; Q32LY0;
DT 04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT 04-MAY-2001, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Serine protease inhibitor Kazal-type 7;
DE AltName: Full=Esophagus cancer-related gene 2 protein;
DE Short=ECRG-2;
DE Flags: Precursor;
GN Name=SPINK7; Synonyms=ECG2; ORFNames=UNQ745/PRO1474;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Esophagus;
RA Bi M., Lu S.;
RT "Cloning and function research of the esophagus cancer related gene-2
RT (ECRG-2).";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP STRUCTURE BY NMR OF 20-85, AND DISULFIDE BONDS.
RX PubMed=22528291; DOI=10.1007/s10858-012-9622-9;
RA Feng Y., Geng Y., Zhou T., Wang J.;
RT "NMR structure note: human esophageal cancer-related gene 2.";
RL J. Biomol. NMR 53:65-70(2012).
CC -!- FUNCTION: Probable serine protease inhibitor.
CC -!- INTERACTION:
CC P58062; P16452: EPB42; NbExp=3; IntAct=EBI-1182445, EBI-1182496;
CC P58062; O15235: MRPS12; NbExp=3; IntAct=EBI-1182445, EBI-712205;
CC P58062; P13640: MT1G; NbExp=3; IntAct=EBI-1182445, EBI-1182473;
CC P58062; P80294: MT1H; NbExp=3; IntAct=EBI-1182445, EBI-296567;
CC P58062; P02795: MT2A; NbExp=7; IntAct=EBI-1182445, EBI-996616;
CC P58062; Q3YEC7: RABL6; NbExp=3; IntAct=EBI-1182445, EBI-742029;
CC P58062; Q8IZW4; NbExp=3; IntAct=EBI-1182445, EBI-1182458;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC Haematology;
CC URL="http://atlasgeneticsoncology.org/Genes/SPINK7ID40396ch5q33.html";
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DR EMBL; AF268198; AAK27795.1; -; mRNA.
DR EMBL; AY359072; AAQ89431.1; -; mRNA.
DR EMBL; BC109385; AAI09386.1; -; mRNA.
DR EMBL; BC110067; AAI10068.1; -; mRNA.
DR CCDS; CCDS4289.1; -.
DR RefSeq; NP_115955.1; NM_032566.2.
DR PDB; 2LEO; NMR; -; A=20-85.
DR PDBsum; 2LEO; -.
DR AlphaFoldDB; P58062; -.
DR BMRB; P58062; -.
DR SMR; P58062; -.
DR BioGRID; 124174; 18.
DR IntAct; P58062; 15.
DR STRING; 9606.ENSP00000274565; -.
DR MEROPS; I01.057; -.
DR BioMuta; SPINK7; -.
DR DMDM; 13959341; -.
DR MassIVE; P58062; -.
DR PaxDb; P58062; -.
DR PeptideAtlas; P58062; -.
DR PRIDE; P58062; -.
DR ProteomicsDB; 57048; -.
DR Antibodypedia; 49020; 84 antibodies from 23 providers.
DR DNASU; 84651; -.
DR Ensembl; ENST00000274565.5; ENSP00000274565.4; ENSG00000145879.11.
DR GeneID; 84651; -.
DR KEGG; hsa:84651; -.
DR MANE-Select; ENST00000274565.5; ENSP00000274565.4; NM_032566.3; NP_115955.1.
DR UCSC; uc003lpd.4; human.
DR CTD; 84651; -.
DR DisGeNET; 84651; -.
DR GeneCards; SPINK7; -.
DR HGNC; HGNC:24643; SPINK7.
DR HPA; ENSG00000145879; Group enriched (esophagus, vagina).
DR neXtProt; NX_P58062; -.
DR OpenTargets; ENSG00000145879; -.
DR PharmGKB; PA143485620; -.
DR VEuPathDB; HostDB:ENSG00000145879; -.
DR eggNOG; KOG3649; Eukaryota.
DR GeneTree; ENSGT00940000161876; -.
DR HOGENOM; CLU_169765_4_1_1; -.
DR InParanoid; P58062; -.
DR OMA; CTVAHFC; -.
DR OrthoDB; 1636538at2759; -.
DR PhylomeDB; P58062; -.
DR PathwayCommons; P58062; -.
DR SignaLink; P58062; -.
DR BioGRID-ORCS; 84651; 10 hits in 1025 CRISPR screens.
DR GenomeRNAi; 84651; -.
DR Pharos; P58062; Tbio.
DR PRO; PR:P58062; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; P58062; protein.
DR Bgee; ENSG00000145879; Expressed in lower esophagus mucosa and 108 other tissues.
DR ExpressionAtlas; P58062; baseline and differential.
DR Genevisible; P58062; HS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR Pfam; PF00050; Kazal_1; 1.
DR SMART; SM00280; KAZAL; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR PROSITE; PS00282; KAZAL_1; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Protease inhibitor; Reference proteome;
KW Secreted; Serine protease inhibitor; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..85
FT /note="Serine protease inhibitor Kazal-type 7"
FT /id="PRO_0000016565"
FT DOMAIN 26..85
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT SITE 47..48
FT /note="Reactive bond"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 32..67
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798,
FT ECO:0000269|PubMed:22528291"
FT DISULFID 45..64
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798,
FT ECO:0000269|PubMed:22528291"
FT DISULFID 53..85
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798,
FT ECO:0000269|PubMed:22528291"
FT HELIX 33..36
FT /evidence="ECO:0007829|PDB:2LEO"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:2LEO"
FT STRAND 59..62
FT /evidence="ECO:0007829|PDB:2LEO"
FT HELIX 63..72
FT /evidence="ECO:0007829|PDB:2LEO"
FT STRAND 75..77
FT /evidence="ECO:0007829|PDB:2LEO"
FT STRAND 79..83
FT /evidence="ECO:0007829|PDB:2LEO"
SQ SEQUENCE 85 AA; 9232 MW; AE861AC8E1EA07D3 CRC64;
MKITGGLLLL CTVVYFCSSS EAASLSPKKV DCSIYKKYPV VAIPCPITYL PVCGSDYITY
GNECHLCTES LKSNGRVQFL HDGSC
