ID   ISK7_MOUSE              Reviewed;          85 AA.
AC   Q6IE32; F8WGH2;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   07-NOV-2018, sequence version 2.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Serine protease inhibitor Kazal-type 7;
DE   AltName: Full=Esophagus cancer-related gene 2 protein;
DE            Short=ECRG-2;
DE   Flags: Precursor;
GN   Name=Spink7; Synonyms=Ecg2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=15060002; DOI=10.1101/gr.1946304;
RA   Puente X.S., Lopez-Otin C.;
RT   "A genomic analysis of rat proteases and protease inhibitors.";
RL   Genome Res. 14:609-622(2004).
CC   -!- FUNCTION: Probable serine protease inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAE51413.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC124383; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BN000361; CAE51413.1; ALT_SEQ; mRNA.
DR   CCDS; CCDS50304.1; -.
DR   RefSeq; NP_001001803.2; NM_001001803.2.
DR   AlphaFoldDB; Q6IE32; -.
DR   SMR; Q6IE32; -.
DR   STRING; 10090.ENSMUSP00000075551; -.
DR   MEROPS; I01.057; -.
DR   PRIDE; Q6IE32; -.
DR   ProteomicsDB; 269511; -.
DR   ProteomicsDB; 372071; -.
DR   Antibodypedia; 49020; 84 antibodies from 23 providers.
DR   DNASU; 408198; -.
DR   Ensembl; ENSMUST00000076194; ENSMUSP00000075551; ENSMUSG00000060201.
DR   GeneID; 408198; -.
DR   KEGG; mmu:408198; -.
DR   CTD; 84651; -.
DR   MGI; MGI:3644691; Spink7.
DR   VEuPathDB; HostDB:ENSMUSG00000060201; -.
DR   eggNOG; KOG3649; Eukaryota.
DR   GeneTree; ENSGT00940000161876; -.
DR   HOGENOM; CLU_169765_4_1_1; -.
DR   InParanoid; Q6IE32; -.
DR   OMA; CTVAHFC; -.
DR   OrthoDB; 1636538at2759; -.
DR   PhylomeDB; Q6IE32; -.
DR   BioGRID-ORCS; 408198; 2 hits in 71 CRISPR screens.
DR   PRO; PR:Q6IE32; -.
DR   Proteomes; UP000000589; Chromosome 18.
DR   RNAct; Q6IE32; protein.
DR   Bgee; ENSMUSG00000060201; Expressed in lip and 8 other tissues.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR002350; Kazal_dom.
DR   InterPro; IPR036058; Kazal_dom_sf.
DR   Pfam; PF00050; Kazal_1; 1.
DR   SMART; SM00280; KAZAL; 1.
DR   SUPFAM; SSF100895; SSF100895; 1.
DR   PROSITE; PS00282; KAZAL_1; 1.
DR   PROSITE; PS51465; KAZAL_2; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Protease inhibitor; Reference proteome; Secreted;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..85
FT                   /note="Serine protease inhibitor Kazal-type 7"
FT                   /id="PRO_0000016566"
FT   DOMAIN          20..85
FT                   /note="Kazal-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   SITE            47..48
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        32..67
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        45..64
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        53..85
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
SQ   SEQUENCE   85 AA;  9282 MW;  DBD3698C07566878 CRC64;
     MKLVGGLLLL FAATYVCNCS EVTSHPSATV DCDIYKKYPV VAIPCPIVNI PVCGSDYITY
     GNKCKLCTEI LRSNGKIQFL HEGHC