ISK8_HUMAN
ID ISK8_HUMAN Reviewed; 97 AA.
AC P0C7L1;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Serine protease inhibitor Kazal-type 8;
DE Flags: Precursor;
GN Name=SPINK8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-97.
RC TISSUE=Myeloid;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probable serine protease inhibitor. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
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DR EMBL; AC105267; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AW467501; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS46822.1; -.
DR RefSeq; NP_001073994.1; NM_001080525.1.
DR AlphaFoldDB; P0C7L1; -.
DR SMR; P0C7L1; -.
DR STRING; 9606.ENSP00000407497; -.
DR GlyGen; P0C7L1; 1 site.
DR BioMuta; SPINK8; -.
DR DMDM; 189044781; -.
DR PaxDb; P0C7L1; -.
DR PRIDE; P0C7L1; -.
DR Antibodypedia; 56531; 67 antibodies from 15 providers.
DR DNASU; 646424; -.
DR Ensembl; ENST00000434006.3; ENSP00000407497.1; ENSG00000229453.3.
DR GeneID; 646424; -.
DR KEGG; hsa:646424; -.
DR MANE-Select; ENST00000434006.3; ENSP00000407497.1; NM_001080525.3; NP_001073994.1.
DR UCSC; uc003csq.2; human.
DR CTD; 646424; -.
DR GeneCards; SPINK8; -.
DR HGNC; HGNC:33160; SPINK8.
DR HPA; ENSG00000229453; Group enriched (bone marrow, breast).
DR neXtProt; NX_P0C7L1; -.
DR OpenTargets; ENSG00000229453; -.
DR PharmGKB; PA162404546; -.
DR VEuPathDB; HostDB:ENSG00000229453; -.
DR eggNOG; ENOG502TDVY; Eukaryota.
DR GeneTree; ENSGT00520000060726; -.
DR HOGENOM; CLU_2346133_0_0_1; -.
DR InParanoid; P0C7L1; -.
DR OMA; YDGPCEN; -.
DR OrthoDB; 1510664at2759; -.
DR PhylomeDB; P0C7L1; -.
DR PathwayCommons; P0C7L1; -.
DR SignaLink; P0C7L1; -.
DR BioGRID-ORCS; 646424; 7 hits in 1064 CRISPR screens.
DR GenomeRNAi; 646424; -.
DR Pharos; P0C7L1; Tdark.
DR PRO; PR:P0C7L1; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; P0C7L1; protein.
DR Bgee; ENSG00000229453; Expressed in lower esophagus mucosa and 76 other tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR Pfam; PF00050; Kazal_1; 1.
DR SMART; SM00280; KAZAL; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Glycoprotein; Protease inhibitor; Reference proteome;
KW Repeat; Secreted; Serine protease inhibitor; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..97
FT /note="Serine protease inhibitor Kazal-type 8"
FT /id="PRO_0000337296"
FT DOMAIN 36..96
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT SITE 51..52
FT /note="Reactive bond"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 42..76
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 49..73
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 62..94
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT VARIANT 78
FT /note="K -> N (in dbSNP:rs11718350)"
FT /id="VAR_043978"
SQ SEQUENCE 97 AA; 10821 MW; 9DEC4480C8E96D7D CRC64;
MKGICSDAIL VLATSMWMAF AIDFPLPMAS ERGQLDKTIV ECLKNVNKCW FLSYIKPSEP
ICGSDQVTYS SDCHLCSKIL FEGLNITKLY DGQCENS
