ID   ISL2A_ONCTS             Reviewed;         358 AA.
AC   P53408;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Insulin gene enhancer protein ISL-2A;
DE            Short=Islet-2A;
GN   Name=isl2a;
OS   Oncorhynchus tshawytscha (Chinook salmon) (Salmo tshawytscha).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC   Salmonidae; Salmoninae; Oncorhynchus.
OX   NCBI_TaxID=74940;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   TISSUE=Pituitary;
RX   PubMed=7852419; DOI=10.1074/jbc.270.7.3335;
RA   Gong Z., Hui C.-C., Hew C.-L.;
RT   "Presence of isl-1-related LIM domain homeobox genes in teleost and their
RT   similar patterns of expression in brain and spinal cord.";
RL   J. Biol. Chem. 270:3335-3345(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND PARTIAL NUCLEOTIDE SEQUENCE
RP   (ISOFORM 2).
RC   TISSUE=Pituitary;
RX   PubMed=7873614; DOI=10.1016/0167-4781(94)00228-u;
RA   Gong Z., Hew C.-L.;
RT   "Several splicing variants of isl-1 like genes in the chinook salmon
RT   (Oncorhynchus tschawytscha) encode truncated transcription factors
RT   containing a complete LIM domain.";
RL   Biochim. Biophys. Acta 1260:349-354(1995).
CC   -!- FUNCTION: Binds to one of the cis-acting domain of the insulin gene
CC       enhancer. May be involved in subtype specialization of primary
CC       motoneurons.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1; Synonyms=Long;
CC         IsoId=P53408-1; Sequence=Displayed;
CC       Name=2; Synonyms=Short;
CC         IsoId=P53408-2; Sequence=VSP_003105, VSP_003106;
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DR   EMBL; X64885; CAA46103.1; ALT_TERM; mRNA.
DR   EMBL; X64882; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; A55973; A55973.
DR   AlphaFoldDB; P53408; -.
DR   BMRB; P53408; -.
DR   SMR; P53408; -.
DR   PRIDE; P53408; -.
DR   Proteomes; UP000694402; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0048513; P:animal organ development; IEA:UniProt.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR001781; Znf_LIM.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00412; LIM; 2.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00132; LIM; 2.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR   PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW   LIM domain; Metal-binding; Nucleus; Reference proteome; Repeat; Zinc.
FT   CHAIN           1..358
FT                   /note="Insulin gene enhancer protein ISL-2A"
FT                   /id="PRO_0000075757"
FT   DOMAIN          27..80
FT                   /note="LIM zinc-binding 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          89..143
FT                   /note="LIM zinc-binding 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DNA_BIND        190..249
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          325..358
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         83..115
FT                   /note="RLFGIKCANCNIGFCSSDLVMRARDNVYHMECF -> SVLGARCAAGISCRG
FT                   MSSLCGTRSCCVELIMVY (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_003105"
FT   VAR_SEQ         116..358
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_003106"
SQ   SEQUENCE   358 AA;  40073 MW;  FB726FE5ACFF25F3 CRC64;
     MVDIIFNSSF LGDMGDHSKK KSGIAMCVGC GSQIHDQYIL RVAPDLEWHA ACLKCAECSQ
     YLDETCTCFV RDGKTYCKRD YVRLFGIKCA NCNIGFCSSD LVMRARDNVY HMECFRCSVC
     SRHLLPGDEF SLRDEELLCR ADHGLLMEQA SAGSPLSPGI IHSRSLHIAD PVSVRQPPHR
     NHVHKQSEKT TRVRTVLNEK QLHTLRTCYN ANPRPDALMK EQLVEMTGLS PRVIRVWFQN
     KRCKDKKRSI FMKQLQQQHH SDKTNLQGLT GTPLVAGSPI RHDNTVQGNP VEVQTYQPPW
     KALSEFALQS DLDQPAFQQL VSFSESGSMG NSSGSDVTSL SSQLPDTPNS MVASPVDT