ISOC2_HUMAN
ID ISOC2_HUMAN Reviewed; 205 AA.
AC Q96AB3; Q6ZN91; Q9H5G0;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Isochorismatase domain-containing protein 2;
GN Name=ISOC2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC TISSUE=Small intestine, and Stomach;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INTERACTION WITH CDKN2A, AND SUBCELLULAR LOCATION.
RX PubMed=17658461; DOI=10.1016/j.bbrc.2007.06.181;
RA Huang X., Shi Z., Wang W., Bai J., Chen Z., Xu J., Zhang D., Fu S.;
RT "Identification and characterization of a novel protein ISOC2 that
RT interacts with p16INK4a.";
RL Biochem. Biophys. Res. Commun. 361:287-293(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA Greff Z., Keri G., Stemmann O., Mann M.;
RT "Kinase-selective enrichment enables quantitative phosphoproteomics of the
RT kinome across the cell cycle.";
RL Mol. Cell 31:438-448(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- SUBUNIT: Interacts with CDKN2A. {ECO:0000269|PubMed:17658461}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17658461}. Nucleus
CC {ECO:0000269|PubMed:17658461}. Note=Localizes to the nucleus in the
CC presence of CDKN2A.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q96AB3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96AB3-2; Sequence=VSP_022000;
CC Name=3;
CC IsoId=Q96AB3-3; Sequence=VSP_038741;
CC -!- SIMILARITY: Belongs to the isochorismatase family. {ECO:0000305}.
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DR EMBL; AK027122; BAB15666.1; -; mRNA.
DR EMBL; AK131324; BAD18484.2; -; mRNA.
DR EMBL; CR457377; CAG33658.1; -; mRNA.
DR EMBL; CH471135; EAW72379.1; -; Genomic_DNA.
DR EMBL; BC017344; AAH17344.1; -; mRNA.
DR CCDS; CCDS12925.1; -. [Q96AB3-2]
DR CCDS; CCDS46194.1; -. [Q96AB3-3]
DR CCDS; CCDS46195.1; -. [Q96AB3-1]
DR RefSeq; NP_001129673.1; NM_001136201.1. [Q96AB3-1]
DR RefSeq; NP_001129674.1; NM_001136202.1. [Q96AB3-3]
DR RefSeq; NP_078986.1; NM_024710.2. [Q96AB3-2]
DR AlphaFoldDB; Q96AB3; -.
DR SMR; Q96AB3; -.
DR BioGRID; 122871; 65.
DR IntAct; Q96AB3; 21.
DR MINT; Q96AB3; -.
DR STRING; 9606.ENSP00000085068; -.
DR iPTMnet; Q96AB3; -.
DR PhosphoSitePlus; Q96AB3; -.
DR BioMuta; ISOC2; -.
DR DMDM; 74731104; -.
DR EPD; Q96AB3; -.
DR jPOST; Q96AB3; -.
DR MassIVE; Q96AB3; -.
DR MaxQB; Q96AB3; -.
DR PeptideAtlas; Q96AB3; -.
DR PRIDE; Q96AB3; -.
DR ProteomicsDB; 75945; -. [Q96AB3-1]
DR ProteomicsDB; 75946; -. [Q96AB3-2]
DR ProteomicsDB; 75947; -. [Q96AB3-3]
DR TopDownProteomics; Q96AB3-1; -. [Q96AB3-1]
DR TopDownProteomics; Q96AB3-2; -. [Q96AB3-2]
DR Antibodypedia; 33099; 87 antibodies from 17 providers.
DR DNASU; 79763; -.
DR Ensembl; ENST00000085068.7; ENSP00000085068.2; ENSG00000063241.8. [Q96AB3-2]
DR Ensembl; ENST00000425675.7; ENSP00000401726.1; ENSG00000063241.8. [Q96AB3-1]
DR Ensembl; ENST00000438389.6; ENSP00000406364.1; ENSG00000063241.8. [Q96AB3-3]
DR GeneID; 79763; -.
DR KEGG; hsa:79763; -.
DR MANE-Select; ENST00000425675.7; ENSP00000401726.1; NM_001136201.2; NP_001129673.1.
DR UCSC; uc002qla.4; human. [Q96AB3-1]
DR CTD; 79763; -.
DR DisGeNET; 79763; -.
DR GeneCards; ISOC2; -.
DR HGNC; HGNC:26278; ISOC2.
DR HPA; ENSG00000063241; Tissue enhanced (liver).
DR MIM; 612928; gene.
DR neXtProt; NX_Q96AB3; -.
DR OpenTargets; ENSG00000063241; -.
DR PharmGKB; PA134981722; -.
DR VEuPathDB; HostDB:ENSG00000063241; -.
DR eggNOG; KOG4044; Eukaryota.
DR GeneTree; ENSGT00390000006753; -.
DR HOGENOM; CLU_1885003_0_0_1; -.
DR InParanoid; Q96AB3; -.
DR OMA; LIVTEHY; -.
DR OrthoDB; 1100720at2759; -.
DR PhylomeDB; Q96AB3; -.
DR TreeFam; TF313459; -.
DR PathwayCommons; Q96AB3; -.
DR SignaLink; Q96AB3; -.
DR BioGRID-ORCS; 79763; 11 hits in 1082 CRISPR screens.
DR GenomeRNAi; 79763; -.
DR Pharos; Q96AB3; Tbio.
DR PRO; PR:Q96AB3; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q96AB3; protein.
DR Bgee; ENSG00000063241; Expressed in right lobe of liver and 144 other tissues.
DR ExpressionAtlas; Q96AB3; baseline and differential.
DR Genevisible; Q96AB3; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:BHF-UCL.
DR GO; GO:0005634; C:nucleus; IDA:BHF-UCL.
DR GO; GO:0031648; P:protein destabilization; IMP:BHF-UCL.
DR Gene3D; 3.40.50.850; -; 1.
DR InterPro; IPR040318; ISOC2.
DR InterPro; IPR000868; Isochorismatase-like.
DR InterPro; IPR036380; Isochorismatase-like_sf.
DR PANTHER; PTHR14119:SF4; PTHR14119:SF4; 1.
DR Pfam; PF00857; Isochorismatase; 1.
DR SUPFAM; SSF52499; SSF52499; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..205
FT /note="Isochorismatase domain-containing protein 2"
FT /id="PRO_0000268671"
FT MOD_RES 7
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18691976"
FT MOD_RES 202
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT VAR_SEQ 47..116
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_038741"
FT VAR_SEQ 116
FT /note="L -> LDPRSYPGLALTSLYPQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039, ECO:0000303|Ref.2"
FT /id="VSP_022000"
SQ SEQUENCE 205 AA; 22337 MW; 2F3B0EFB2C7B705F CRC64;
MAAARPSLGR VLPGSSVLFL CDMQEKFRHN IAYFPQIVSV AARMLKVARL LEVPVMLTEQ
YPQGLGPTVP ELGTEGLRPL AKTCFSMVPA LQQELDSRPQ LRSVLLCGIE AQACILNTTL
DLLDRGLQVH VVVDACSSRS QVDRLVALAR MRQSGAFLST SEGLILQLVG DAVHPQFKEI
QKLIKEPAPD SGLLGLFQGQ NSLLH
