ID   ISP1_BACSU              Reviewed;         319 AA.
AC   P11018; O34477;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Major intracellular serine protease;
DE            EC=3.4.21.-;
DE   AltName: Full=ISP-1;
DE   Flags: Precursor;
GN   Name=isp; Synonyms=ispA; OrderedLocusNames=BSU13190;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, SUBUNIT, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=NBRC 3013;
RX   PubMed=3087947; DOI=10.1128/jb.167.1.110-116.1986;
RA   Koide Y., Nakamura A., Uozumi T., Beppu T.;
RT   "Cloning and sequencing of the major intracellular serine protease gene of
RT   Bacillus subtilis.";
RL   J. Bacteriol. 167:110-116(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Devine K.M.;
RT   "Sequence of the Bacillus subtilis genome between xlyA and ykoR.";
RL   Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- FUNCTION: Major intracellular protease produced by Bacillus subtilis.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:3087947}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:3087947}.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR   EMBL; M13760; AAA22557.1; -; Genomic_DNA.
DR   EMBL; AJ002571; CAA05598.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB13176.1; -; Genomic_DNA.
DR   PIR; I39866; I39866.
DR   RefSeq; NP_389202.1; NC_000964.3.
DR   RefSeq; WP_003232562.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; P11018; -.
DR   SMR; P11018; -.
DR   STRING; 224308.BSU13190; -.
DR   MEROPS; S08.030; -.
DR   PaxDb; P11018; -.
DR   PRIDE; P11018; -.
DR   EnsemblBacteria; CAB13176; CAB13176; BSU_13190.
DR   GeneID; 939934; -.
DR   KEGG; bsu:BSU13190; -.
DR   PATRIC; fig|224308.179.peg.1433; -.
DR   eggNOG; COG1404; Bacteria.
DR   InParanoid; P11018; -.
DR   OMA; APEMWAK; -.
DR   PhylomeDB; P11018; -.
DR   BioCyc; BSUB:BSU13190-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07477; Peptidases_S8_Subtilisin_subset; 1.
DR   Gene3D; 3.40.50.200; -; 1.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR   InterPro; IPR022398; Peptidase_S8_His-AS.
DR   InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   InterPro; IPR034202; Subtilisin_Carlsberg-like.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF52743; SSF52743; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00136; SUBTILASE_ASP; 1.
DR   PROSITE; PS00137; SUBTILASE_HIS; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Hydrolase; Protease;
KW   Reference proteome; Serine protease; Zymogen.
FT   PROPEP          1..17
FT                   /id="PRO_0000027038"
FT   CHAIN           18..319
FT                   /note="Major intracellular serine protease"
FT                   /id="PRO_0000027039"
FT   DOMAIN          23..307
FT                   /note="Peptidase S8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        50
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        87
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        246
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   CONFLICT        71
FT                   /note="T -> S (in Ref. 1; AAA22557)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        160
FT                   /note="K -> E (in Ref. 1; AAA22557)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   319 AA;  33849 MW;  3517855FC57AA5ED CRC64;
     MNGEIRLIPY VTNEQIMDVN ELPEGIKVIK APEMWAKGVK GKNIKVAVLD TGCDTSHPDL
     KNQIIGGKNF TDDDGGKEDA ISDYNGHGTH VAGTIAANDS NGGIAGVAPE ASLLIVKVLG
     GENGSGQYEW IINGINYAVE QKVDIISMSL GGPSDVPELK EAVKNAVKNG VLVVCAAGNE
     GDGDERTEEL SYPAAYNEVI AVGSVSVARE LSEFSNANKE IDLVAPGENI LSTLPNKKYG
     KLTGTSMAAP HVSGALALIK SYEEESFQRK LSESEVFAQL IRRTLPLDIA KTLAGNGFLY
     LTAPDELAEK AEQSHLLTL