ID   ISP6_SCHPO              Reviewed;         467 AA.
AC   P40903;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 144.
DE   RecName: Full=Sexual differentiation process putative subtilase-type proteinase isp6;
DE            EC=3.4.21.-;
GN   Name=isp6; ORFNames=SPAC4A8.04;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7954893; DOI=10.1007/bf00326301;
RA   Sato S., Suzuki H., Widyastuti U., Hotta Y., Tabata S.;
RT   "Identification and characterization of genes induced during sexual
RT   differentiation in Schizosaccharomyces pombe.";
RL   Curr. Genet. 26:31-37(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- DEVELOPMENTAL STAGE: Transcribed specifically during sexual
CC       development.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR   EMBL; D14063; BAA03149.1; -; mRNA.
DR   EMBL; CU329670; CAB11474.1; -; Genomic_DNA.
DR   PIR; S45493; S45493.
DR   RefSeq; NP_593815.1; NM_001019245.2.
DR   AlphaFoldDB; P40903; -.
DR   SMR; P40903; -.
DR   BioGRID; 279529; 10.
DR   STRING; 4896.SPAC4A8.04.1; -.
DR   MEROPS; S08.A54; -.
DR   MaxQB; P40903; -.
DR   PaxDb; P40903; -.
DR   EnsemblFungi; SPAC4A8.04.1; SPAC4A8.04.1:pep; SPAC4A8.04.
DR   GeneID; 2543097; -.
DR   KEGG; spo:SPAC4A8.04; -.
DR   PomBase; SPAC4A8.04; isp6.
DR   VEuPathDB; FungiDB:SPAC4A8.04; -.
DR   eggNOG; KOG1153; Eukaryota.
DR   HOGENOM; CLU_011263_1_4_1; -.
DR   InParanoid; P40903; -.
DR   OMA; SNYGKCN; -.
DR   PhylomeDB; P40903; -.
DR   Reactome; R-SPO-8866427; VLDLR internalisation and degradation.
DR   Reactome; R-SPO-8964038; LDL clearance.
DR   PRO; PR:P40903; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0000324; C:fungal-type vacuole; IDA:PomBase.
DR   GO; GO:0000328; C:fungal-type vacuole lumen; EXP:PomBase.
DR   GO; GO:0005794; C:Golgi apparatus; HDA:PomBase.
DR   GO; GO:0031906; C:late endosome lumen; EXP:PomBase.
DR   GO; GO:0004175; F:endopeptidase activity; IMP:PomBase.
DR   GO; GO:0008233; F:peptidase activity; IMP:PomBase.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IDA:PomBase.
DR   GO; GO:0006914; P:autophagy; IMP:PomBase.
DR   GO; GO:0000747; P:conjugation with cellular fusion; IMP:PomBase.
DR   GO; GO:0007033; P:vacuole organization; IMP:PomBase.
DR   GO; GO:0031638; P:zymogen activation; IMP:PomBase.
DR   CDD; cd04077; Peptidases_S8_PCSK9_ProteinaseK_like; 1.
DR   Gene3D; 3.30.70.80; -; 1.
DR   Gene3D; 3.40.50.200; -; 1.
DR   InterPro; IPR034193; PCSK9_ProteinaseK-like.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR   InterPro; IPR022398; Peptidase_S8_His-AS.
DR   InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   InterPro; IPR010259; S8pro/Inhibitor_I9.
DR   InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR   Pfam; PF05922; Inhibitor_I9; 1.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF52743; SSF52743; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00136; SUBTILASE_ASP; 1.
DR   PROSITE; PS00137; SUBTILASE_HIS; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Protease; Reference proteome; Serine protease.
FT   CHAIN           1..467
FT                   /note="Sexual differentiation process putative subtilase-
FT                   type proteinase isp6"
FT                   /id="PRO_0000076410"
FT   DOMAIN          86..176
FT                   /note="Inhibitor I9"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          186..467
FT                   /note="Peptidase S8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        221
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        253
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        409
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
SQ   SEQUENCE   467 AA;  49367 MW;  A60A0EDF62ADF721 CRC64;
     MRIPYSNLFS AAAGLALFAS TACAAPVMPA TDSDIAHAGI RPELDNAFYD SHGEAATPKH
     KPHAGPNAAP LLSASNADTT GLDSHYIIVL QPDLSEQEFQ AHTNWVSEMH QMDIASQEDE
     YYDTSDSNYM FGLKHVYDFG EDSFKGYSGQ FSSNIVEQIR LHPHVIAVER DQVVSIKKLE
     TQSGAPWGLA RISHKSVKYD DIGKYVYDSS AGDNITAYVV DTGVSIHHVE FEGRASWGAT
     IPSGDVDEDN NGHGTHVAGT IASRAYGVAK KAEIVAVKVL RSSGSGTMAD VIAGVEWTVR
     HHKSSGKKTS VGNMSLGGGN SFVLDMAVDS AVTNGVIYAV AAGNEYDDAC YSSPAASKKA
     ITVGASTIND QMAYFSNYGS CVDIFAPGLN ILSTWIGSNT STNTISGTSM ATPHVAGLSA
     YYLGLHPAAS ASEVKDAIIK MGIHDVLLSI PVGSSTINLL AFNGAQE