ID   4HYPE_STAND             Reviewed;         333 AA.
AC   D7A0Y3;
DT   24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT   10-AUG-2010, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=4-hydroxyproline 2-epimerase {ECO:0000303|PubMed:25608448};
DE            Short=4Hyp 2-epimerase;
DE            Short=4HypE {ECO:0000303|PubMed:25608448};
DE            EC=5.1.1.8 {ECO:0000269|PubMed:25608448};
GN   OrderedLocusNames=Snov_0157 {ECO:0000312|EMBL:ADH87493.1};
OS   Starkeya novella (strain ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM
OS   12100 / NBRC 12443 / NCIMB 10456).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Xanthobacteraceae; Starkeya.
OX   NCBI_TaxID=639283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM 12100 / NBRC 12443
RC   / NCIMB 10456;
RX   PubMed=23450099; DOI=10.4056/sigs.3006378;
RA   Kappler U., Davenport K., Beatson S., Lucas S., Lapidus A., Copeland A.,
RA   Berry K.W., Glavina Del Rio T., Hammon N., Dalin E., Tice H., Pitluck S.,
RA   Richardson P., Bruce D., Goodwin L.A., Han C., Tapia R., Detter J.C.,
RA   Chang Y.J., Jeffries C.D., Land M., Hauser L., Kyrpides N.C., Goker M.,
RA   Ivanova N., Klenk H.P., Woyke T.;
RT   "Complete genome sequence of the facultatively chemolithoautotrophic and
RT   methylotrophic alpha Proteobacterium Starkeya novella type strain (ATCC
RT   8093(T)).";
RL   Stand. Genomic Sci. 7:44-58(2012).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM 12100 / NBRC 12443
RC   / NCIMB 10456;
RX   PubMed=25608448; DOI=10.1021/ja5103986;
RA   Zhang X., Kumar R., Vetting M.W., Zhao S., Jacobson M.P., Almo S.C.,
RA   Gerlt J.A.;
RT   "A unique cis-3-hydroxy-L-proline dehydratase in the enolase superfamily.";
RL   J. Am. Chem. Soc. 137:1388-1391(2015).
CC   -!- FUNCTION: Catalyzes the epimerization of trans-4-hydroxy-L-proline
CC       (t4LHyp) to cis-4-hydroxy-D-proline (c4DHyp). May be involved in a
CC       degradation pathway of t4LHyp, which would allow S.novella to grow on
CC       t4LHyp as a sole carbon source. {ECO:0000269|PubMed:25608448}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline;
CC         Xref=Rhea:RHEA:21152, ChEBI:CHEBI:57690, ChEBI:CHEBI:58375;
CC         EC=5.1.1.8; Evidence={ECO:0000269|PubMed:25608448};
CC   -!- SIMILARITY: Belongs to the proline racemase family. {ECO:0000305}.
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DR   EMBL; CP002026; ADH87493.1; -; Genomic_DNA.
DR   RefSeq; WP_013164998.1; NC_014217.1.
DR   AlphaFoldDB; D7A0Y3; -.
DR   SMR; D7A0Y3; -.
DR   STRING; 639283.Snov_0157; -.
DR   EnsemblBacteria; ADH87493; ADH87493; Snov_0157.
DR   KEGG; sno:Snov_0157; -.
DR   eggNOG; COG3938; Bacteria.
DR   HOGENOM; CLU_036729_0_0_5; -.
DR   OMA; SHVLWTG; -.
DR   OrthoDB; 559014at2; -.
DR   Proteomes; UP000006633; Chromosome.
DR   GO; GO:0047580; F:4-hydroxyproline epimerase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR008794; Pro_racemase_fam.
DR   PANTHER; PTHR33442; PTHR33442; 1.
DR   Pfam; PF05544; Pro_racemase; 1.
DR   PIRSF; PIRSF029792; Pro_racemase; 1.
DR   SFLD; SFLDS00028; Proline_Racemase; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Reference proteome.
FT   CHAIN           1..333
FT                   /note="4-hydroxyproline 2-epimerase"
FT                   /id="PRO_0000433402"
FT   ACT_SITE        90
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT   ACT_SITE        253
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT   BINDING         91..92
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT   BINDING         223
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT   BINDING         249
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KGU2"
FT   BINDING         254..255
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KGU2"
SQ   SEQUENCE   333 AA;  36144 MW;  64A2E0FBE32FD42D CRC64;
     MARHSFFCID GHTCGNPVRL VAGGGPNLQG ANMIEKRAHF LAEYDWIRTG LMFEPRGHDM
     MSGSILYPPT RPDCDVAILF IETSGCLPMC GHGTIGTVTM AIEHGLVTPK IPGVLMLDTP
     AGVVKAEYRQ EGQYVEEVRI TNVPAFLYAR GLTAECPGLG EVMVDVAYGG NFYAIVEPQE
     HFRDMADFTA GELIGMSGAL RKALNAKYEF VHPEKPEIRG LSHILWTGAP KHAEAHARNA
     VFYGDKAIDR SPCGTGTSAR IAHWAANGKL KVGDDFVHES IIGSLFKGRV EATARVGNVD
     AIIPSIGGWA RMTGYNTIFI DDRDPFAHGF VVV