ISPG_ORYSJ
ID ISPG_ORYSJ Reviewed; 744 AA.
AC Q6K8J4; A0A0P0VLD4; Q84PA7;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin), chloroplastic;
DE EC=1.17.7.1;
DE AltName: Full=1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase;
DE Flags: Precursor;
GN Name=ISPG; OrderedLocusNames=Os02g0603800, LOC_Os02g39160;
GN ORFNames=OJ1669_F01.30, OsJ_07430;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 137-744.
RX PubMed=12684538; DOI=10.1073/pnas.0737574100;
RA Cooper B., Clarke J.D., Budworth P., Kreps J., Hutchison D., Park S.,
RA Guimil S., Dunn M., Luginbuehl P., Ellero C., Goff S.A., Glazebrook J.;
RT "A network of rice genes associated with stress response and seed
RT development.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:4945-4950(2003).
CC -!- FUNCTION: Enzyme of the plastid non-mevalonate pathway for isoprenoid
CC biosynthesis that converts 2-C-methyl-D-erythritol 2,4-cyclodiphosphate
CC (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Is
CC essential for chloroplast development (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + H2O + 2
CC oxidized [2Fe-2S]-[ferredoxin] = 2-C-methyl-D-erythritol 2,4-cyclic
CC diphosphate + H(+) + 2 reduced [2Fe-2S]-[ferredoxin];
CC Xref=Rhea:RHEA:26119, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33737,
CC ChEBI:CHEBI:33738, ChEBI:CHEBI:58483, ChEBI:CHEBI:128753;
CC EC=1.17.7.1;
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 1 [4Fe-4S] cluster per subunit. {ECO:0000250};
CC -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis
CC via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-
CC phosphate: step 5/6.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the IspG family. {ECO:0000305}.
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DR EMBL; AP004124; BAD19354.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF09279.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS79642.1; -; Genomic_DNA.
DR EMBL; CM000139; EEE57327.1; -; Genomic_DNA.
DR EMBL; AK120769; BAH00163.1; -; mRNA.
DR EMBL; AY224457; AAO72576.1; -; mRNA.
DR RefSeq; XP_015625724.1; XM_015770238.1.
DR AlphaFoldDB; Q6K8J4; -.
DR SMR; Q6K8J4; -.
DR IntAct; Q6K8J4; 1.
DR STRING; 4530.OS02T0603800-01; -.
DR PaxDb; Q6K8J4; -.
DR PRIDE; Q6K8J4; -.
DR EnsemblPlants; Os02t0603800-01; Os02t0603800-01; Os02g0603800.
DR GeneID; 4329911; -.
DR Gramene; Os02t0603800-01; Os02t0603800-01; Os02g0603800.
DR KEGG; osa:4329911; -.
DR eggNOG; ENOG502QSBY; Eukaryota.
DR HOGENOM; CLU_012689_0_0_1; -.
DR InParanoid; Q6K8J4; -.
DR OMA; YGYVGAG; -.
DR OrthoDB; 868852at2759; -.
DR PlantReactome; R-OSA-1119464; Methylerythritol phosphate pathway.
DR UniPathway; UPA00056; UER00096.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6K8J4; OS.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046429; F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity; IBA:GO_Central.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway; IBA:GO_Central.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:InterPro.
DR Gene3D; 3.20.20.20; -; 1.
DR Gene3D; 3.30.413.10; -; 1.
DR HAMAP; MF_00159; IspG; 1.
DR InterPro; IPR011005; Dihydropteroate_synth-like.
DR InterPro; IPR017178; IspG_atypical.
DR InterPro; IPR004588; IspG_bac-typ.
DR InterPro; IPR045854; NO2/SO3_Rdtase_4Fe4S_sf.
DR PANTHER; PTHR30454; PTHR30454; 1.
DR Pfam; PF04551; GcpE; 1.
DR PIRSF; PIRSF037336; IspG_like; 1.
DR SUPFAM; SSF56014; SSF56014; 1.
DR TIGRFAMs; TIGR00612; ispG_gcpE; 1.
PE 2: Evidence at transcript level;
KW 4Fe-4S; Chloroplast; Iron; Iron-sulfur; Isoprene biosynthesis;
KW Metal-binding; Oxidoreductase; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..39
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 40..744
FT /note="4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
FT (ferredoxin), chloroplastic"
FT /id="PRO_0000417591"
FT BINDING 648
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 651
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 682
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 689
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
SQ SEQUENCE 744 AA; 82161 MW; 6BE6F2134FC0604F CRC64;
MATGVAPAPL PHVRVRDGGI GFTRSVDFAK ILSVPATLRV GSSRGRVLVA KSSSTGSDTM
ELEPSSEGSP LLVPRQKYCE SIYETRRRKT RTVMVGNVPL GSDHPIRIQT MTTSDTKDVA
KTVEEVMRIA DKGADFVRIT VQGRKEADAC FEIKNTLVQK NYNIPLVADI HFAPTVALRV
AECFDKIRVN PGNFADRRAQ FEQLEYTEDD YQKELEHIEK VFSPLVEKCK QYGRAMRIGT
NHGSLSDRIM SYYGDSPRGM VESALEFARI CRKLDFHNFV FSMKASNPVI MVQAYRLLVA
EMYNLGWDYP LHLGVTEAGE GEDGRMKSAI GIGTLLMDGL GDTIRVSLTE PPEEEIDPCR
RLANLGTHAA DLQIGVAPFE EKHRRYFDFQ RRSGQLPLQK EGEEVDYRGV LHRDGSVLMS
VSLDQLKAPE LLYRSLAAKL VVGMPFKDLA TVDSILLREL PPVEDAQARL ALKRLVDISM
GVLTPLSEQL TKPLPHAIAL VNVDELSSGA HKLLPEGTRL AVTLRGDESY EQLDLLKGVD
DITMLLHSVP YGEEKTGRVH AARRLFEYLE TNGLNFPVIH HIEFPKSVNR DDLVIGAGAN
VGALLVDGLG DGVLLEAADQ EFEFLRDTSF NLLQGCRMRN TKTEYVSCPS CGRTLFDLQE
VSAQIREKTS HLPGVSIAIM GCIVNGPGEM ADADFGYVGG APGKIDLYVG KTVVQRGIAM
EGATDALIQL IKDHGRWVDP PVEE
