ID   ISPI2_GALME             Reviewed;          52 AA.
AC   P81906;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Inducible serine protease inhibitor 2;
DE            Short=ISPI-2;
DE   Flags: Fragment;
OS   Galleria mellonella (Greater wax moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Pyraloidea;
OC   Pyralidae; Galleriinae; Galleria.
OX   NCBI_TaxID=7137;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Hemolymph;
RX   PubMed=10727944; DOI=10.1046/j.1432-1327.2000.01207.x;
RA   Froebius A.C., Kanost M.R., Goetz P., Vilcinskas A.;
RT   "Isolation and characterization of novel inducible serine protease
RT   inhibitors from larval hemolymph of the greater wax moth Galleria
RT   mellonella.";
RL   Eur. J. Biochem. 267:2046-2053(2000).
CC   -!- FUNCTION: Inhibits trypsin and the toxin protease PR2 of M.anisopliae.
CC       Does not inhibit chymotrypsin, subtilisin Carlsberg, proteinase K,
CC       porcine pancreatic elastase and the toxin protease PR1 of M.anisopliae.
CC   -!- DEVELOPMENTAL STAGE: Last instar larvae.
CC   -!- INDUCTION: By infection.
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DR   AlphaFoldDB; P81906; -.
DR   SMR; P81906; -.
DR   MEROPS; I02.958; -.
DR   Proteomes; UP000504614; Unplaced.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Reference proteome; Serine protease inhibitor.
FT   CHAIN           1..>52
FT                   /note="Inducible serine protease inhibitor 2"
FT                   /id="PRO_0000155428"
FT   DOMAIN          5..>52
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   SITE            15..16
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   DISULFID        14..38
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        30..51
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   NON_TER         52
SQ   SEQUENCE   52 AA;  6057 MW;  31CED34D59C42ABE CRC64;
     LDPKCTLPLE TGICRAELHR FGYDTKLKEC TQFVYGGCHH NENNFKKLEV CR