ISPS_POPTM
ID ISPS_POPTM Reviewed; 595 AA.
AC Q7XAS7;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Isoprene synthase, chloroplastic;
DE EC=4.2.3.27;
DE Flags: Precursor;
GN Name=ISPS;
OS Populus tremuloides (Quaking aspen).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus.
OX NCBI_TaxID=3693;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=15653811; DOI=10.1104/pp.104.054445;
RA Sharkey T.D., Yeh S., Wiberley A.E., Falbel T.G., Gong D., Fernandez D.E.;
RT "Evolution of the isoprene biosynthetic pathway in kudzu.";
RL Plant Physiol. 137:700-712(2005).
CC -!- FUNCTION: Lyase that catalyzes the formation of isoprene from
CC dimethylallyl diphosphate.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dimethylallyl diphosphate = diphosphate + isoprene;
CC Xref=Rhea:RHEA:13369, ChEBI:CHEBI:33019, ChEBI:CHEBI:35194,
CC ChEBI:CHEBI:57623; EC=4.2.3.27;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsb subfamily.
CC {ECO:0000305}.
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DR EMBL; AY341431; AAQ16588.1; -; Genomic_DNA.
DR AlphaFoldDB; Q7XAS7; -.
DR SMR; Q7XAS7; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0034009; F:isoprene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 3: Inferred from homology;
KW Chloroplast; Lyase; Magnesium; Metal-binding; Plastid; Transit peptide.
FT TRANSIT 1..37
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 38..595
FT /note="Isoprene synthase, chloroplastic"
FT /id="PRO_0000398181"
FT MOTIF 345..349
FT /note="DDXXD motif"
FT BINDING 345
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 345
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 349
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 349
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 489
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 493
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 497
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
SQ SEQUENCE 595 AA; 68446 MW; A55977EDD7F6BBAA CRC64;
MATELLCLHR PISLTHKLFR NPLPKVIQAT PLTLKLRCSV STENVSFSET ETETRRSANY
EPNSWDYDYL LSSDTDESIE VHKDKAKKLE AEVRREINNE KAEFLTLLEL IDNVQRLGLG
YRFESDIRRA LDRFVSSGGF DGVTKTSLHG TALSFRLLRQ HGFEVSQEAF SGFKDQNGNF
LENLKEDIKA ILSLYEASFL ALEGENILDE AKVFAISHLK ELSEEKIGKE LAEQVSHALE
LPLHRRTQRL EAVWSIEAYR KKEDANQVLL ELAILDYNMI QSVYQRDLRE TSRWWRRVGL
ATKLHFARDR LIESFYWAVG VAFEPQYSDC RNSVAKMFSF VTIIDDIYDV YGTLDELELF
TDAVERWDVN AINDLPDYMK LCFLALYNTI NEIAYDNLKD KGENILPYLT KAWADLCNAF
LQEAKWLYNK STPTFDDYFG NAWKSSSGPL QLIFAYFAVV QNIKKEEIEN LQKYHDIISR
PSHIFRLCND LASASAEIAR GETANSVSCY MRTKGISEEL ATESVMNLID ETWKKMNKEK
LGGSLFAKPF VETAINLARQ SHCTYHNGDA HTSPDELTRK RVLSVITEPI LPFER
