位置:首页 > 蛋白库 > ISPS_PUEML
ISPS_PUEML
ID   ISPS_PUEML              Reviewed;         608 AA.
AC   Q6EJ97;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Isoprene synthase, chloroplastic;
DE            EC=4.2.3.27;
DE   Flags: Precursor;
GN   Name=ISPS;
OS   Pueraria montana var. lobata (Kudzu vine) (Pueraria lobata).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Pueraria.
OX   NCBI_TaxID=3893;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=15653811; DOI=10.1104/pp.104.054445;
RA   Sharkey T.D., Yeh S., Wiberley A.E., Falbel T.G., Gong D., Fernandez D.E.;
RT   "Evolution of the isoprene biosynthetic pathway in kudzu.";
RL   Plant Physiol. 137:700-712(2005).
CC   -!- FUNCTION: Lyase that catalyzes the formation of isoprene from
CC       dimethylallyl diphosphate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dimethylallyl diphosphate = diphosphate + isoprene;
CC         Xref=Rhea:RHEA:13369, ChEBI:CHEBI:33019, ChEBI:CHEBI:35194,
CC         ChEBI:CHEBI:57623; EC=4.2.3.27;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=7.7 mM for dimethylallyl diphosphate
CC         {ECO:0000269|PubMed:15653811};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast. Note=recovered from
CC       purified chloroplasts of transgenic Arabidopsis plants.
CC   -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC       the catalytic activity, presumably through binding to Mg(2+).
CC   -!- MISCELLANEOUS: Sufficient to confer the trait of isoprene emission when
CC       expressed in Arabidopsis.
CC   -!- SIMILARITY: Belongs to the terpene synthase family. Tpsb subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY316691; AAQ84170.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6EJ97; -.
DR   SMR; Q6EJ97; -.
DR   BRENDA; 4.2.3.27; 13960.
DR   SABIO-RK; Q6EJ97; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0034009; F:isoprene synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR   InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Lyase; Magnesium; Metal-binding; Plastid; Transit peptide.
FT   TRANSIT         1..45
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           46..608
FT                   /note="Isoprene synthase, chloroplastic"
FT                   /id="PRO_0000398182"
FT   MOTIF           350..354
FT                   /note="DDXXD motif"
FT   BINDING         350
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         350
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         354
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         354
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         497
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   BINDING         501
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   BINDING         505
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   608 AA;  70075 MW;  2BE7005A49BB418A CRC64;
     MATNLLCLSN KLSSPTPTPS TRFPQSKNFI TQKTSLANPK PWRVICATSS QFTQITEHNS
     RRSANYQPNL WNFEFLQSLE NDLKVEKLEE KATKLEEEVR CMINRVDTQP LSLLELIDDV
     QRLGLTYKFE KDIIKALENI VLLDENKKNK SDLHATALSF RLLRQHGFEV SQDVFERFKD
     KEGGFSGELK GDVQGLLSLY EASYLGFEGE NLLEEARTFS ITHLKNNLKE GINTKVAEQV
     SHALELPYHQ RLHRLEARWF LDKYEPKEPH HQLLLELAKL DFNMVQTLHQ KELQDLSRWW
     TEMGLASKLD FVRDRLMEVY FWALGMAPDP QFGECRKAVT KMFGLVTIID DVYDVYGTLD
     ELQLFTDAVE RWDVNAINTL PDYMKLCFLA LYNTVNDTSY SILKEKGHNN LSYLTKSWRE
     LCKAFLQEAK WSNNKIIPAF SKYLENASVS SSGVALLAPS YFSVCQQQED ISDHALRSLT
     DFHGLVRSSC VIFRLCNDLA TSAAELERGE TTNSIISYMH ENDGTSEEQA REELRKLIDA
     EWKKMNRERV SDSTLLPKAF MEIAVNMARV SHCTYQYGDG LGRPDYATEN RIKLLLIDPF
     PINQLMYV
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024