ID   4OT2_PSEPU              Reviewed;          63 AA.
AC   Q8RQD2;
DT   10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=2-hydroxymuconate tautomerase;
DE            EC=5.3.2.6;
DE   AltName: Full=4-oxalocrotonate tautomerase;
DE            Short=4-OT;
GN   Name=xylH;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid TOL pDK1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PaW630;
RA   Wessler H.G., Hares D.R., Poulter M.D., Voss J.A., Khedairy H.S.,
RA   Baker R.F., Azadpour E.E., Luo X., Benjamin R.C.;
RT   "Sequence of the lower (meta-cleavage) xyl operon of the Pseudomonas putida
RT   TOL plasmid pDK1.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the ketonization of 2-hydroxymuconate
CC       stereoselectively to yield 2-oxo-3-hexenedioate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-
CC         enedioate; Xref=Rhea:RHEA:33431, ChEBI:CHEBI:28080,
CC         ChEBI:CHEBI:64908; EC=5.3.2.6;
CC   -!- PATHWAY: Xenobiotic degradation; toluene degradation.
CC   -!- PATHWAY: Xenobiotic degradation; xylene degradation.
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the 4-oxalocrotonate tautomerase family.
CC       {ECO:0000305}.
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DR   EMBL; AF134348; AAL83667.1; -; Genomic_DNA.
DR   RefSeq; WP_003454301.1; NZ_MING01000087.1.
DR   RefSeq; YP_003617191.1; NC_014124.1.
DR   RefSeq; YP_709354.1; NC_008275.1.
DR   AlphaFoldDB; Q8RQD2; -.
DR   SMR; Q8RQD2; -.
DR   UniPathway; UPA00228; -.
DR   UniPathway; UPA00273; -.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042203; P:toluene catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0042184; P:xylene catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00491; 4Oxalocrotonate_Tautomerase; 1.
DR   Gene3D; 3.30.429.10; -; 1.
DR   InterPro; IPR004370; 4-oxalocrotonate_tautomerase.
DR   InterPro; IPR014347; Tautomerase/MIF_sf.
DR   InterPro; IPR018191; Tautomerase_Pseudo-typ.
DR   Pfam; PF01361; Tautomerase; 1.
DR   SUPFAM; SSF55331; SSF55331; 1.
DR   TIGRFAMs; TIGR00013; taut; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Isomerase; Plasmid.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..63
FT                   /note="2-hydroxymuconate tautomerase"
FT                   /id="PRO_0000209515"
FT   ACT_SITE        2
FT                   /note="Proton acceptor; via imino nitrogen"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   63 AA;  6969 MW;  23805E3F916B5802 CRC64;
     MPIAQIHILE GRNDEQKETL IREVSEAISR SLDAPLTSVR VIITEMAKGH FGIGGELASK
     VRR