ID   ISP_ALKCL               Reviewed;         321 AA.
AC   P29140;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Intracellular alkaline protease;
DE            EC=3.4.21.-;
OS   Alkalihalobacillus clausii (Bacillus clausii).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus.
OX   NCBI_TaxID=79880;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 21522 / DSM 2512 / JCM 9139 / LMG 18518 / 221;
RA   Kato C., Nakano Y., Yamamoto M., Horikoshi K.;
RT   "Cloning and nucleotide sequence of the intracellular alkaline protease
RT   gene of alkaliphilic Bacillus sp. strain 221.";
RL   Submitted (MAR-1992) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR   EMBL; D10730; BAA01573.1; -; Genomic_DNA.
DR   PIR; S27501; S27501.
DR   AlphaFoldDB; P29140; -.
DR   SMR; P29140; -.
DR   MEROPS; S08.030; -.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07477; Peptidases_S8_Subtilisin_subset; 1.
DR   Gene3D; 3.40.50.200; -; 1.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR   InterPro; IPR022398; Peptidase_S8_His-AS.
DR   InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   InterPro; IPR034202; Subtilisin_Carlsberg-like.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF52743; SSF52743; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00136; SUBTILASE_ASP; 1.
DR   PROSITE; PS00137; SUBTILASE_HIS; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Serine protease.
FT   CHAIN           1..321
FT                   /note="Intracellular alkaline protease"
FT                   /id="PRO_0000076408"
FT   DOMAIN          22..311
FT                   /note="Peptidase S8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        49
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        86
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        250
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
SQ   SEQUENCE   321 AA;  33747 MW;  621168D9F1044026 CRC64;
     MRKFRLIPYK QVDKVSALSE VPMGVEIVEA PAVWKASAKG AGQIIGVIDT GCQVDHPDLA
     ERIIGGVNLT TDYGGVETNF SDNNGHGTHV AGTVAAAETG SGVVGVAPKA DLFIIKALSG
     DGSGEMGWIA KAIRYAVDWR GPKGEQMRII TMSLGGPTDS EELHDAVKYA VSNNVSVVCA
     AGNEGDGRED TNEFAYPAAY NEVIAVGAVD FDLRLSDFPN TNEEIDIVAP GVGIKSTYLD
     SGYAELSGTS MAAPHVAGAL ALIINLAKDA FKRTLSETEI CAQLVRRATP IGFTAQDKGN
     GFLTLGLVER ITGQFTEKGK K