IST1_PONAB
ID IST1_PONAB Reviewed; 364 AA.
AC Q5R6G8;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 25-MAY-2022, entry version 61.
DE RecName: Full=IST1 homolog;
DE AltName: Full=Charged multivesicular body protein 8 {ECO:0000250|UniProtKB:P53990};
DE Short=CHMP8 {ECO:0000250|UniProtKB:P53990};
GN Name=IST1;
OS Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pongo.
OX NCBI_TaxID=9601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart;
RG The German cDNA consortium;
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: ESCRT-III-like protein involved in cytokinesis, nuclear
CC envelope reassembly and endosomal tubulation (By similarity). Is
CC required for efficient abscission during cytokinesis (By similarity).
CC Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing
CC cells (By similarity). During late anaphase, involved in nuclear
CC envelope reassembly and mitotic spindle disassembly together with the
CC ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to
CC the nuclear membrane, leading to microtubule severing (By similarity).
CC Recruited to the reforming nuclear envelope (NE) during anaphase by
CC LEMD2 (By similarity). Regulates early endosomal tubulation together
CC with the ESCRT-III complex by mediating the recruitment of SPAST (By
CC similarity). {ECO:0000250|UniProtKB:P53990}.
CC -!- SUBUNIT: Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with
CC SPAST, STAMBP, and USP8. May interact with VPS37B. May associate with
CC the ESCRT-I complex. Interacts with MITD1, in competition with VSP4.
CC Interacts with SPART (via MIT domain); leading to the recruitment of
CC SPART to midbodies. Interacts with SPAST.
CC {ECO:0000250|UniProtKB:P53990}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle
CC {ECO:0000250|UniProtKB:P53990}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome {ECO:0000250|UniProtKB:P53990}. Midbody
CC {ECO:0000250|UniProtKB:P53990}. Nucleus envelope
CC {ECO:0000250|UniProtKB:P53990}. Note=Localizes to centrosome and
CC midbody of dividing cells. Colocalized with SPART to the ends of
CC Flemming bodies during cytokinesis. Localizes to the reforming nuclear
CC envelope on chromatin disks during late anaphase.
CC {ECO:0000250|UniProtKB:P53990}.
CC -!- SIMILARITY: Belongs to the IST1 family. {ECO:0000305}.
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DR EMBL; CR860522; CAH92648.1; -; mRNA.
DR RefSeq; NP_001126550.1; NM_001133078.1.
DR AlphaFoldDB; Q5R6G8; -.
DR SMR; Q5R6G8; -.
DR STRING; 9601.ENSPPYP00000008494; -.
DR GeneID; 100173540; -.
DR KEGG; pon:100173540; -.
DR CTD; 9798; -.
DR eggNOG; KOG2027; Eukaryota.
DR InParanoid; Q5R6G8; -.
DR Proteomes; UP000001595; Unplaced.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR GO; GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
DR GO; GO:0005635; C:nuclear envelope; IEA:UniProtKB-SubCell.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:InterPro.
DR Gene3D; 1.20.1260.60; -; 1.
DR InterPro; IPR005061; Ist1.
DR InterPro; IPR042277; IST1-like.
DR PANTHER; PTHR12161; PTHR12161; 1.
DR Pfam; PF03398; Ist1; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..364
FT /note="IST1 homolog"
FT /id="PRO_0000274479"
FT REGION 294..352
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 4
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53990"
FT MOD_RES 43
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P53990"
SQ SEQUENCE 364 AA; 39662 MW; 0BE160A5CB2520E8 CRC64;
MLGSGFKAER LRVNLRLVIN RLKLLEKKKT ELAQKARKEI ADYLAAGKDE RARIRVEHII
REDYLVEAME ILELYCDLLL ARFGLIQSMK ELDSGLAESV STLIWAAPRL QSEVAELKIV
ADQLCAKYSK GYGKLCRTNQ IGTVNDRLMH KLSVEAPPKI LVERYLIEIA KNYNVPYEPD
SVVMAEAPPG VETDLIDVGF TDDVKKGGPG RGGGGGFTAP VGGPEGTVPM PMPMPMPSAN
TPFSYPLPKG PSDFNGLPMG TYQAFPIIHP PQIPATPPSY ESVDDINADK NISSAQIVGP
GPKPEASAKL PSRPADNYDN FVLPELPSVP DTLPTASAGA STSASEDIDF DDLSRRFEEL
KKKT