IST1_SCHPO
ID IST1_SCHPO Reviewed; 271 AA.
AC O74490;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Vacuolar protein sorting-associated protein ist1;
GN Name=ist1; ORFNames=SPCC1442.17c, SPCC285.02c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Involved in a late step in sorting of cargo proteins of the
CC multivesicular body (MVB) for incorporation into intralumenal vesicles.
CC The lumenal sequestrated membrane proteins are targeted into the
CC vacuole after fusion of the endosome with the vacuole (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372}. Golgi apparatus
CC {ECO:0000269|PubMed:16823372}. Endosome {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the IST1 family. {ECO:0000305}.
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DR EMBL; CU329672; CAA21451.1; -; Genomic_DNA.
DR PIR; T40982; T40982.
DR RefSeq; NP_588331.1; NM_001023322.2.
DR AlphaFoldDB; O74490; -.
DR SMR; O74490; -.
DR BioGRID; 275577; 19.
DR STRING; 4896.SPCC1442.17c.1; -.
DR iPTMnet; O74490; -.
DR MaxQB; O74490; -.
DR PaxDb; O74490; -.
DR PRIDE; O74490; -.
DR EnsemblFungi; SPCC1442.17c.1; SPCC1442.17c.1:pep; SPCC1442.17c.
DR PomBase; SPCC1442.17c; ist1.
DR VEuPathDB; FungiDB:SPCC1442.17c; -.
DR eggNOG; KOG2027; Eukaryota.
DR HOGENOM; CLU_1034981_0_0_1; -.
DR InParanoid; O74490; -.
DR OMA; TLAQDNK; -.
DR PhylomeDB; O74490; -.
DR Reactome; R-SPO-6798695; Neutrophil degranulation.
DR Reactome; R-SPO-9668328; Sealing of the nuclear envelope (NE) by ESCRT-III.
DR PRO; PR:O74490; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005768; C:endosome; ISO:PomBase.
DR GO; GO:0005794; C:Golgi apparatus; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0008104; P:protein localization; IBA:GO_Central.
DR GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; ISO:PomBase.
DR Gene3D; 1.20.1260.60; -; 1.
DR InterPro; IPR005061; Ist1.
DR InterPro; IPR042277; IST1-like.
DR PANTHER; PTHR12161; PTHR12161; 1.
DR Pfam; PF03398; Ist1; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Endosome; Golgi apparatus; Nucleus; Reference proteome.
FT CHAIN 1..271
FT /note="Vacuolar protein sorting-associated protein ist1"
FT /id="PRO_0000274480"
FT REGION 189..255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 196..211
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 220..255
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 271 AA; 30808 MW; 276A2FC266CFDD91 CRC64;
MSRLQIQLKL AASRIEILRQ KEEALAKQAR RNVALGLKSY SPALAKARIE PLIMQDIYIE
LLELLQVDVE ILANRCVVLE KRAFNDSMSF KSSLYHVMAA APQLQIKELR FVHDFLVKLY
GKEFARLSDP DLATNDTAFY QLLYPPIPKD ELVEAYRKEL IRTYFPNDYP KEANKDIPSS
AEADTMLNLE SSENREQPTS LKEENTQEDD EKVNGNLANI SKRAEPQNNG STNASSLQSS
NYLPTNPTLT HSNKAPSFEE LAARLDRLKH L
