ID   ISTA_PSEAI              Reviewed;         390 AA.
AC   P15025;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2001, sequence version 2.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Transposase for insertion sequence element IS21;
GN   Name=istA;
OS   Pseudomonas aeruginosa.
OG   Plasmid R68.45.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2540414; DOI=10.1007/bf00427038;
RA   Reimmann C., Moore R., Little S., Savioz A., Willetts N.S., Haas D.;
RT   "Genetic structure, function and regulation of the transposable element
RT   IS21.";
RL   Mol. Gen. Genet. 215:416-424(1989).
RN   [2]
RP   SEQUENCE REVISION TO 283.
RA   Berger B.;
RL   Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the transposition of the insertion sequence.
CC   -!- SIMILARITY: Belongs to the transposase IS21/IS408/IS1162 family.
CC       {ECO:0000305}.
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DR   EMBL; X14793; CAA32898.2; -; Genomic_DNA.
DR   PIR; JV0012; BVECIS.
DR   AlphaFoldDB; P15025; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR017894; HTH_IS21_transposase_type.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00665; rve; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50531; HTH_IS21; 1.
DR   PROSITE; PS50994; INTEGRASE; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Plasmid; Transposable element;
KW   Transposition.
FT   CHAIN           1..390
FT                   /note="Transposase for insertion sequence element IS21"
FT                   /id="PRO_0000075460"
FT   DOMAIN          5..66
FT                   /note="HTH IS21-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00615"
FT   DOMAIN          111..285
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
FT   DNA_BIND        20..39
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00615"
SQ   SEQUENCE   390 AA;  45983 MW;  826B0037237C9070 CRC64;
     MLSREDFYMI KQMRQQGAYI VDIATQIGCS ERTVRRYLKY PEPPARKTRH KMVKLKPFMD
     YIDMRLAENV WNSEVIFAEI KAMGYTGGRS MLRYYIQPKR KMRPSKRTVR FETQPRYQLQ
     HDWGEVEVEV AGQRCKVNFA VNTLGFSRRF HVFAAPKQDA EHTYESLVRA FRYFGGCVKT
     VLVDNQKAAV LKNNNGKVVF NSGFLLLADH YNFLPRACRP RRARTKGKVE RMVKYLKENF
     FVRYRRFDSF THVNQQLEQW IADVADKREL RQFKETPEQR FALEQEHLQP LPDTDFDTSY
     FDIRHVSWDS YIEVGGNRYS VPEALCGQPV SIRISLDDEL RIYSNEKLVA SHRLCSASSG
     WQTVPEHHAP LWQQVSQVEH RPLSAYEELL