ID   ISU1_ASHGO              Reviewed;         154 AA.
AC   Q75C07;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Iron sulfur cluster assembly protein 1, mitochondrial;
DE   AltName: Full=Iron sulfur cluster scaffold protein 1;
DE   Flags: Precursor;
GN   Name=ISU1; OrderedLocusNames=ACR112C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Scaffold protein for the de novo synthesis of iron-sulfur
CC       (Fe-S) clusters within mitochondria, which is required for maturation
CC       of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins.
CC       First, a [2Fe-2S] cluster is transiently assembled on the scaffold
CC       protein ISU1. In a second step, the cluster is released from ISU1,
CC       transferred to a glutaredoxin, followed by the formation of
CC       mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and
CC       their target-specific insertion into the recipient apoproteins. Cluster
CC       assembly on ISU1 depends on the function of the cysteine desulfurase
CC       complex NFS1-ISD11, which serves as the sulfur donor for cluster
CC       synthesis, the iron-binding protein frataxin as the putative iron
CC       donor, and the electron transfer chain comprised of ferredoxin
CC       reductase and ferredoxin, which receive their electrons from NADH.
CC       {ECO:0000250|UniProtKB:Q03020}.
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC         Evidence={ECO:0000250|UniProtKB:Q9UTC6};
CC       Note=Binds 1 [2Fe-2S] cluster per subunit.
CC       {ECO:0000250|UniProtKB:Q9UTC6};
CC   -!- PATHWAY: Cofactor biosynthesis; iron-sulfur cluster biosynthesis.
CC       {ECO:0000250|UniProtKB:Q03020}.
CC   -!- SUBUNIT: Component of the core Fe-S cluster (ISC) assembly machinery.
CC       {ECO:0000250|UniProtKB:Q03020}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix
CC       {ECO:0000250|UniProtKB:Q03020}.
CC   -!- SIMILARITY: Belongs to the NifU family. {ECO:0000305}.
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DR   EMBL; AE016816; AAS51338.1; -; Genomic_DNA.
DR   RefSeq; NP_983514.1; NM_208867.1.
DR   AlphaFoldDB; Q75C07; -.
DR   SMR; Q75C07; -.
DR   STRING; 33169.AAS51338; -.
DR   EnsemblFungi; AAS51338; AAS51338; AGOS_ACR112C.
DR   GeneID; 4619645; -.
DR   KEGG; ago:AGOS_ACR112C; -.
DR   eggNOG; KOG3361; Eukaryota.
DR   HOGENOM; CLU_079283_5_0_1; -.
DR   InParanoid; Q75C07; -.
DR   OMA; YKNPRNY; -.
DR   UniPathway; UPA00266; -.
DR   Proteomes; UP000000591; Chromosome III.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IBA:GO_Central.
DR   GO; GO:0008198; F:ferrous iron binding; IBA:GO_Central.
DR   GO; GO:0006879; P:cellular iron ion homeostasis; IBA:GO_Central.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR   CDD; cd06664; IscU_like; 1.
DR   InterPro; IPR011339; ISC_FeS_clus_asmbl_IscU.
DR   InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR   PANTHER; PTHR10093; PTHR10093; 1.
DR   Pfam; PF01592; NifU_N; 1.
DR   TIGRFAMs; TIGR01999; iscU; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Iron; Iron-sulfur; Metal-binding; Mitochondrion;
KW   Reference proteome; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..154
FT                   /note="Iron sulfur cluster assembly protein 1,
FT                   mitochondrial"
FT                   /id="PRO_0000019694"
SQ   SEQUENCE   154 AA;  16812 MW;  AC7DDC049733F9DF CRC64;
     MLGFRQTATA IVRPVGTIGY ARRCYHPKVI DHYTNPRNVG TLDKKLTNVG TGLVGAPACG
     DVMRLQIQVD DSTGVIENVK FKTFGCGSAI ASSSYMTELV RGKTLADAEK IKNTEIAREL
     SLPPVKLHCS MLAEDAIKAA IKDYRSKRKA TELR