ID   ISU1_ENCCU              Reviewed;         140 AA.
AC   Q8SSM2;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Iron sulfur cluster assembly protein 1;
DE   AltName: Full=Iron sulfur cluster scaffold protein 1;
GN   Name=ISU1; OrderedLocusNames=ECU01_0510;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11157783; DOI=10.1101/gr.164301;
RA   Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M.,
RA   Weissenbach J., Saurin W., Vivares C.P.;
RT   "Sequence and analysis of chromosome I of the amitochondriate intracellular
RT   parasite Encephalitozoon cuniculi (Microspora).";
RL   Genome Res. 11:198-207(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
RN   [3]
RP   SUBCELLULAR LOCATION, AND FUNCTION.
RX   PubMed=18311129; DOI=10.1038/nature06606;
RA   Goldberg A.V., Molik S., Tsaousis A.D., Neumann K., Kuhnke G., Delbac F.,
RA   Vivares C.P., Hirt R.P., Lill R., Embley T.M.;
RT   "Localization and functionality of microsporidian iron-sulphur cluster
RT   assembly proteins.";
RL   Nature 452:624-628(2008).
CC   -!- FUNCTION: Scaffold protein for the de novo synthesis of iron-sulfur
CC       (Fe-S) clusters within mitosomes, which is required for maturation of
CC       both [2Fe-2S] and [4Fe-4S] proteins (PubMed:18311129). First, a [2Fe-
CC       2S] cluster is transiently assembled on the scaffold protein ISU1. In a
CC       second step, the cluster is released from ISU1, transferred to a
CC       glutaredoxin, followed by the formation of [2Fe-2S] proteins, the
CC       synthesis of [4Fe-4S] clusters and their target-specific insertion into
CC       the recipient apoproteins. Cluster assembly on ISU1 depends on the
CC       function of the cysteine desulfurase complex NFS1-ISD11, which serves
CC       as the sulfur donor for cluster synthesis, the iron-binding protein
CC       frataxin as the putative iron donor, and the electron transfer chain
CC       comprised of ferredoxin reductase and ferredoxin, which receive their
CC       electrons from NADH (By similarity). {ECO:0000250|UniProtKB:Q03020,
CC       ECO:0000269|PubMed:18311129}.
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC         Evidence={ECO:0000250|UniProtKB:Q9UTC6};
CC       Note=Binds 1 [2Fe-2S] cluster per subunit.
CC       {ECO:0000250|UniProtKB:Q9UTC6};
CC   -!- PATHWAY: Cofactor biosynthesis; iron-sulfur cluster biosynthesis.
CC       {ECO:0000250|UniProtKB:Q03020}.
CC   -!- SUBUNIT: Component of the core Fe-S cluster (ISC) assembly machinery.
CC       {ECO:0000250|UniProtKB:Q03020}.
CC   -!- SUBCELLULAR LOCATION: Mitosome matrix {ECO:0000269|PubMed:18311129}.
CC   -!- SIMILARITY: Belongs to the NifU family. {ECO:0000305}.
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DR   EMBL; AL391737; CAD24920.1; -; Genomic_DNA.
DR   RefSeq; XP_965885.1; XM_960792.1.
DR   AlphaFoldDB; Q8SSM2; -.
DR   SMR; Q8SSM2; -.
DR   STRING; 284813.Q8SSM2; -.
DR   GeneID; 860225; -.
DR   KEGG; ecu:ECU01_0510; -.
DR   VEuPathDB; MicrosporidiaDB:ECU01_0510; -.
DR   HOGENOM; CLU_079283_5_0_1; -.
DR   InParanoid; Q8SSM2; -.
DR   OMA; HKKVIDH; -.
DR   OrthoDB; 1406557at2759; -.
DR   UniPathway; UPA00266; -.
DR   Proteomes; UP000000819; Chromosome I.
DR   GO; GO:0032047; C:mitosome; IEA:UniProtKB-KW.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR   CDD; cd06664; IscU_like; 1.
DR   InterPro; IPR011339; ISC_FeS_clus_asmbl_IscU.
DR   InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR   PANTHER; PTHR10093; PTHR10093; 1.
DR   Pfam; PF01592; NifU_N; 1.
DR   TIGRFAMs; TIGR01999; iscU; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Iron; Iron-sulfur; Metal-binding; Mitosome; Reference proteome.
FT   CHAIN           1..140
FT                   /note="Iron sulfur cluster assembly protein 1"
FT                   /id="PRO_0000382932"
SQ   SEQUENCE   140 AA;  15095 MW;  8DCA8EDE8C65F4A9 CRC64;
     MSALQELSGI TGKYDSSVVD HFENPRNVGS LDKTDPRVGT GMVGAPACGD VMKLQIKVGK
     DNVIEDAKFK TFGCGSAIAS SSLATEWIKK KTIDESLKIS NRDIAKKLSL PPIKLHCSML
     AEDAIKMAIK DFLDKNKPSP