ISU1_ORYSJ
ID ISU1_ORYSJ Reviewed; 171 AA.
AC Q8LR34;
DT 13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Iron-sulfur cluster assembly protein 1;
DE Short=OsIsu1 {ECO:0000303|PubMed:19396402};
DE Flags: Precursor;
GN Name=ISU1 {ECO:0000303|PubMed:19396402};
GN OrderedLocusNames=Os01g0662600 {ECO:0000312|EMBL:BAF05697.1},
GN LOC_Os01g47340 {ECO:0000305};
GN ORFNames=P0671D01.28 {ECO:0000312|EMBL:BAB91740.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=19396402; DOI=10.1007/s10529-009-0004-7;
RA Tsugama D., Liu S., Takano T.;
RT "Stage- and tissue-specific expression of rice OsIsu1 gene encoding a
RT scaffold protein for mitochondrial iron-sulfur-cluster biogenesis.";
RL Biotechnol. Lett. 31:1305-1310(2009).
CC -!- FUNCTION: Scaffold protein for the de novo synthesis of iron-sulfur
CC (Fe-S) clusters within mitochondria, which is required for maturation
CC of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins
CC (Probable). First, a [2Fe-2S] cluster is transiently assembled on the
CC scaffold protein ISCU (ISU1, ISU2 or ISU3). In a second step, the
CC cluster is released from ISCU, transferred to a glutaredoxin, followed
CC by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of
CC [4Fe-4S] clusters and their target-specific insertion into the
CC recipient apoproteins. Cluster assembly on ISCU depends on the function
CC of the cysteine desulfurase complex NFS1-ISD11, which serves as the
CC sulfur donor for cluster synthesis, the iron-binding protein frataxin
CC as the putative iron donor, and the electron transfer chain comprised
CC of ferredoxin reductase and ferredoxin, which receive their electrons
CC from NADH (By similarity). {ECO:0000250|UniProtKB:Q03020,
CC ECO:0000305|PubMed:19396402}.
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000250|UniProtKB:O49627};
CC Note=Binds 1 [2Fe-2S] cluster per subunit.
CC {ECO:0000250|UniProtKB:O49627};
CC -!- PATHWAY: Cofactor biosynthesis; iron-sulfur cluster biosynthesis.
CC {ECO:0000250|UniProtKB:Q03020}.
CC -!- SUBUNIT: Component of the core Fe-S cluster (ISC) assembly machinery.
CC {ECO:0000250|UniProtKB:Q03020}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix
CC {ECO:0000305|PubMed:19396402}.
CC -!- INDUCTION: Down-regulated in roots when grown under iron deficiency
CC conditions. {ECO:0000269|PubMed:19396402}.
CC -!- SIMILARITY: Belongs to the NifU family. {ECO:0000305}.
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DR EMBL; AP003284; BAB91740.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF05697.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS73546.1; -; Genomic_DNA.
DR EMBL; AK062234; BAG88251.1; -; mRNA.
DR EMBL; AK098887; BAG93794.1; -; mRNA.
DR RefSeq; XP_015621640.1; XM_015766154.1.
DR AlphaFoldDB; Q8LR34; -.
DR SMR; Q8LR34; -.
DR STRING; 4530.OS01T0662600-02; -.
DR PaxDb; Q8LR34; -.
DR PRIDE; Q8LR34; -.
DR EnsemblPlants; Os01t0662600-01; Os01t0662600-01; Os01g0662600.
DR EnsemblPlants; Os01t0662600-02; Os01t0662600-02; Os01g0662600.
DR GeneID; 4327858; -.
DR Gramene; Os01t0662600-01; Os01t0662600-01; Os01g0662600.
DR Gramene; Os01t0662600-02; Os01t0662600-02; Os01g0662600.
DR KEGG; osa:4327858; -.
DR eggNOG; KOG3361; Eukaryota.
DR HOGENOM; CLU_079283_1_2_1; -.
DR InParanoid; Q8LR34; -.
DR OMA; YKNPRNY; -.
DR OrthoDB; 1406557at2759; -.
DR UniPathway; UPA00266; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR ExpressionAtlas; Q8LR34; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005759; C:mitochondrial matrix; IDA:UniProtKB.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0008198; F:ferrous iron binding; IBA:GO_Central.
DR GO; GO:0044571; P:[2Fe-2S] cluster assembly; IDA:UniProtKB.
DR GO; GO:0044572; P:[4Fe-4S] cluster assembly; IDA:UniProtKB.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IBA:GO_Central.
DR CDD; cd06664; IscU_like; 1.
DR InterPro; IPR011339; ISC_FeS_clus_asmbl_IscU.
DR InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR PANTHER; PTHR10093; PTHR10093; 1.
DR Pfam; PF01592; NifU_N; 1.
DR TIGRFAMs; TIGR01999; iscU; 1.
PE 2: Evidence at transcript level;
KW 2Fe-2S; Iron; Iron-sulfur; Metal-binding; Mitochondrion;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..55
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 56..171
FT /note="Iron-sulfur cluster assembly protein 1"
FT /id="PRO_0000446287"
SQ SEQUENCE 171 AA; 17944 MW; DB3FCC2DF44FD5EA CRC64;
MLRAGGRRLL APGLRRVLGG GAAAPVAVGG AKAYHERVVD HYENPRNVGS FENDDPSVGT
GLVGAPACGD VMKLQIRVDE SSGKIVDACF KTFGCGSAIA SSSVATEWVK GKQMEEVVTI
KNTEIAKHLS LPPVKLHCSM LAEDAIKAAV KDYEAKKAKL AQKGEEKAAE A
