ID   ISU1_TRAHO              Reviewed;         138 AA.
AC   B0YLW7; L7JS96;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 44.
DE   RecName: Full=Iron sulfur cluster assembly protein 1;
DE   AltName: Full=Iron sulfur cluster scaffold protein 1;
GN   Name=ISU1; ORFNames=THOM_2800;
OS   Trachipleistophora hominis (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Pleistophoridae;
OC   Trachipleistophora.
OX   NCBI_TaxID=72359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND FUNCTION.
RX   PubMed=18311129; DOI=10.1038/nature06606;
RA   Goldberg A.V., Molik S., Tsaousis A.D., Neumann K., Kuhnke G., Delbac F.,
RA   Vivares C.P., Hirt R.P., Lill R., Embley T.M.;
RT   "Localization and functionality of microsporidian iron-sulphur cluster
RT   assembly proteins.";
RL   Nature 452:624-628(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23133373; DOI=10.1371/journal.ppat.1002979;
RA   Heinz E., Williams T.A., Nakjang S., Noel C.J., Swan D.C., Goldberg A.V.,
RA   Harris S.R., Weinmaier T., Markert S., Becher D., Bernhardt J., Dagan T.,
RA   Hacker C., Lucocq J.M., Schweder T., Rattei T., Hall N., Hirt R.P.,
RA   Embley T.M.;
RT   "The genome of the obligate intracellular parasite Trachipleistophora
RT   hominis: new insights into microsporidian genome dynamics and reductive
RT   evolution.";
RL   PLoS Pathog. 8:E1002979-E1002979(2012).
CC   -!- FUNCTION: Scaffold protein for the de novo synthesis of iron-sulfur
CC       (Fe-S) clusters within mitosomes, which is required for maturation of
CC       both [2Fe-2S] and [4Fe-4S] proteins (PubMed:18311129). First, a [2Fe-
CC       2S] cluster is transiently assembled on the scaffold protein ISU1. In a
CC       second step, the cluster is released from ISU1, transferred to a
CC       glutaredoxin, followed by the formation of [2Fe-2S] proteins, the
CC       synthesis of [4Fe-4S] clusters and their target-specific insertion into
CC       the recipient apoproteins. Cluster assembly on ISU1 depends on the
CC       function of the cysteine desulfurase complex NFS1-ISD11, which serves
CC       as the sulfur donor for cluster synthesis, the iron-binding protein
CC       frataxin as the putative iron donor, and the electron transfer chain
CC       comprised of ferredoxin reductase and ferredoxin, which receive their
CC       electrons from NADH (By similarity). {ECO:0000250|UniProtKB:Q03020,
CC       ECO:0000269|PubMed:18311129}.
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC         Evidence={ECO:0000250|UniProtKB:Q9UTC6};
CC       Note=Binds 1 [2Fe-2S] cluster per subunit.
CC       {ECO:0000250|UniProtKB:Q9UTC6};
CC   -!- PATHWAY: Cofactor biosynthesis; iron-sulfur cluster biosynthesis.
CC       {ECO:0000250|UniProtKB:Q03020}.
CC   -!- SUBUNIT: Component of the core Fe-S cluster (ISC) assembly machinery.
CC       {ECO:0000250|UniProtKB:Q03020}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18311129}. Note=It
CC       is unknown how the different localizations of several ISC components to
CC       mitosomes and cytoplasm can be reconciled with the usually tightly
CC       coordinated function of these components.
CC       {ECO:0000305|PubMed:18311129}.
CC   -!- SIMILARITY: Belongs to the NifU family. {ECO:0000305}.
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DR   EMBL; EF571314; ABS58598.1; -; Genomic_DNA.
DR   EMBL; JH994054; ELQ74294.1; -; Genomic_DNA.
DR   AlphaFoldDB; B0YLW7; -.
DR   SMR; B0YLW7; -.
DR   STRING; 72359.B0YLW7; -.
DR   EnsemblFungi; ELQ74294; ELQ74294; THOM_2800.
DR   VEuPathDB; MicrosporidiaDB:THOM_2800; -.
DR   HOGENOM; CLU_079283_5_0_1; -.
DR   OMA; YKNPRNY; -.
DR   UniPathway; UPA00266; -.
DR   Proteomes; UP000011185; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR   CDD; cd06664; IscU_like; 1.
DR   InterPro; IPR011339; ISC_FeS_clus_asmbl_IscU.
DR   InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR   PANTHER; PTHR10093; PTHR10093; 1.
DR   Pfam; PF01592; NifU_N; 1.
DR   TIGRFAMs; TIGR01999; iscU; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Cytoplasm; Iron; Iron-sulfur; Metal-binding; Reference proteome.
FT   CHAIN           1..138
FT                   /note="Iron sulfur cluster assembly protein 1"
FT                   /id="PRO_0000382933"
SQ   SEQUENCE   138 AA;  15064 MW;  7F868AB2BEAC13E8 CRC64;
     MLTPFNNFFQ QRQNYHSSVI DHFENPRNVG SYDKSANDVG TGLVGAPACG DVMKLQIKVE
     NDIIKDAKFR TFGCGSAIAS SSLATEWIKG KSISDSLKIT NKDIAKKLSL PPVKLHCSML
     AEDAIKAAIS DYQKKNGL