ID   ISY1_CAEEL              Reviewed;         267 AA.
AC   Q20716;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Protein isy-1 {ECO:0000305};
GN   Name=isy-1 {ECO:0000312|WormBase:F53B7.3};
GN   ORFNames=F53B7.3 {ECO:0000312|WormBase:F53B7.3};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE,
RP   AND MUTAGENESIS OF GLU-180.
RX   PubMed=29664006; DOI=10.7554/elife.35037;
RA   Jiang W., Wei Y., Long Y., Owen A., Wang B., Wu X., Luo S., Dang Y.,
RA   Ma D.K.;
RT   "A genetic program mediates cold-warming response and promotes stress-
RT   induced phenoptosis in C. elegans.";
RL   Elife 7:0-0(2018).
CC   -!- FUNCTION: Regulates the processing of the mir-60 microRNA (miRNA),
CC       which in turn negatively regulates the expression of the transcription
CC       factor zip-10 (PubMed:29664006). Does not affect the splicing of zip-10
CC       (PubMed:29664006). {ECO:0000269|PubMed:29664006}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:29664006}.
CC   -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC       {ECO:0000269|PubMed:29664006}.
CC   -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in up-regulation
CC       of the transcription factor zip-10 and its downstream target asp-17.
CC       {ECO:0000269|PubMed:29664006}.
CC   -!- SIMILARITY: Belongs to the ISY1 family. {ECO:0000305}.
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DR   EMBL; BX284605; CAA96652.1; -; Genomic_DNA.
DR   PIR; T22548; T22548.
DR   RefSeq; NP_505803.1; NM_073402.4.
DR   AlphaFoldDB; Q20716; -.
DR   IntAct; Q20716; 1.
DR   STRING; 6239.F53B7.3.1; -.
DR   EPD; Q20716; -.
DR   PaxDb; Q20716; -.
DR   PeptideAtlas; Q20716; -.
DR   EnsemblMetazoa; F53B7.3.1; F53B7.3.1; WBGene00009966.
DR   GeneID; 179524; -.
DR   KEGG; cel:CELE_F53B7.3; -.
DR   UCSC; F53B7.3; c. elegans.
DR   CTD; 179524; -.
DR   WormBase; F53B7.3; CE05922; WBGene00009966; isy-1.
DR   eggNOG; KOG3068; Eukaryota.
DR   GeneTree; ENSGT00390000014109; -.
DR   HOGENOM; CLU_043453_0_1_1; -.
DR   InParanoid; Q20716; -.
DR   OMA; IPSCEKW; -.
DR   OrthoDB; 1513531at2759; -.
DR   PhylomeDB; Q20716; -.
DR   Reactome; R-CEL-6781823; Formation of TC-NER Pre-Incision Complex.
DR   Reactome; R-CEL-6782135; Dual incision in TC-NER.
DR   Reactome; R-CEL-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR   Reactome; R-CEL-72163; mRNA Splicing - Major Pathway.
DR   PRO; PR:Q20716; -.
DR   Proteomes; UP000001940; Chromosome V.
DR   Bgee; WBGene00009966; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0071014; C:post-mRNA release spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0071020; C:post-spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR   GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IBA:GO_Central.
DR   GO; GO:0000389; P:mRNA 3'-splice site recognition; IBA:GO_Central.
DR   GO; GO:2000634; P:regulation of primary miRNA processing; IMP:UniProtKB.
DR   Gene3D; 1.10.287.660; -; 1.
DR   InterPro; IPR029012; Helix_hairpin_bin_sf.
DR   InterPro; IPR009360; Isy1.
DR   InterPro; IPR037200; Isy1_sf.
DR   PANTHER; PTHR13021; PTHR13021; 1.
DR   Pfam; PF06246; Isy1; 1.
DR   SUPFAM; SSF140102; SSF140102; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Nucleus; Reference proteome.
FT   CHAIN           1..267
FT                   /note="Protein isy-1"
FT                   /id="PRO_0000448070"
FT   REGION          195..221
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          175..204
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         180
FT                   /note="E->K: In dma50; up-regulates expression of the
FT                   transcription factor zip-10 and its downstream target asp-
FT                   17."
FT                   /evidence="ECO:0000269|PubMed:29664006"
SQ   SEQUENCE   267 AA;  31271 MW;  900C378DEBACB4C9 CRC64;
     MARNAEKAMT ALARWRRMKE EEERGPIARR PHDVKDCRNL SDAERFRREI VRDASKKITA
     IQNPGLGEFK LRDLNDEVNR LIKLKHAWEQ RIRELGGTDY RKYAQKELDA IGRETGNSRG
     YKYFGAAKDL PGVRELFEKS TEGEEQRRHR ADLLRNIDAH YFGYLDDEDG RLIPLEKLIE
     EKNIERINKE FAEKQAQKQQ TASDAAPENI YKVEEDDDDD LETQESTVIG EDGRPMTIRH
     VLLPTQQDIE EMLLEQKKQE LMAKYLD