ID   ISY1_CRYNJ              Reviewed;         348 AA.
AC   P0CO36; Q55U67; Q5KI78;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=Pre-mRNA-splicing factor ISY1;
GN   Name=ISY1; OrderedLocusNames=CND03910;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Involved in pre-mRNA splicing. {ECO:0000250}.
CC   -!- SUBUNIT: Associated with the spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ISY1 family. {ECO:0000305}.
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DR   EMBL; AE017344; AAW43321.1; -; Genomic_DNA.
DR   RefSeq; XP_570628.1; XM_570628.1.
DR   AlphaFoldDB; P0CO36; -.
DR   STRING; 5207.AAW43321; -.
DR   PaxDb; P0CO36; -.
DR   EnsemblFungi; AAW43321; AAW43321; CND03910.
DR   GeneID; 3257141; -.
DR   KEGG; cne:CND03910; -.
DR   VEuPathDB; FungiDB:CND03910; -.
DR   eggNOG; KOG3068; Eukaryota.
DR   HOGENOM; CLU_043453_0_1_1; -.
DR   InParanoid; P0CO36; -.
DR   OMA; NQGPAYF; -.
DR   OrthoDB; 1513531at2759; -.
DR   Proteomes; UP000002149; Chromosome 4.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0071014; C:post-mRNA release spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0071020; C:post-spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR   GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IBA:GO_Central.
DR   GO; GO:0000389; P:mRNA 3'-splice site recognition; IBA:GO_Central.
DR   Gene3D; 1.10.287.660; -; 1.
DR   InterPro; IPR029012; Helix_hairpin_bin_sf.
DR   InterPro; IPR009360; Isy1.
DR   InterPro; IPR037200; Isy1_sf.
DR   PANTHER; PTHR13021; PTHR13021; 1.
DR   Pfam; PF06246; Isy1; 1.
DR   SUPFAM; SSF140102; SSF140102; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Spliceosome.
FT   CHAIN           1..348
FT                   /note="Pre-mRNA-splicing factor ISY1"
FT                   /id="PRO_0000192967"
FT   REGION          220..282
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        236..278
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   348 AA;  39124 MW;  59ACF65790D407C5 CRC64;
     MARNSEKAQS MLYRFREQQA IDMGIGTRQK GDRRPRMASS CTSLREAERW RGDILRDISR
     KVSKIQDVAL TDYQVRDLND EINQLFREKR AWENQIINLG GANYRRAAGV MTDDEGREVP
     GTRGYKYFGR AKELPGVKEL FTRSTQQATE ESARTASFQM FRHQGPDYYG DEDELDKELI
     DTEDVEAREG WEDQVRKSAS TLGISDETLL PRYPVSISSK LPDASVDPTQ LQENEEAPQK
     SEKALKGTGK SKRKYKGVND LEEGGQKEEQ EEAKKSKTDT SVAINSVAEG QNIASETAVA
     AAQAQAAAFL GVLDAESLEF PTMPSKDEMA KVLLEVRKQA LKEEYGVY