ID   ISY1_DICDI              Reviewed;         299 AA.
AC   Q54N41;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Pre-mRNA-splicing factor ISY1 homolog;
GN   Name=isy1; ORFNames=DDB_G0285521;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: May play a role in pre-mRNA splicing. {ECO:0000250}.
CC   -!- SUBUNIT: Identified in the spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ISY1 family. {ECO:0000305}.
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DR   EMBL; AAFI02000079; EAL64598.1; -; Genomic_DNA.
DR   RefSeq; XP_638102.1; XM_633010.1.
DR   AlphaFoldDB; Q54N41; -.
DR   SMR; Q54N41; -.
DR   STRING; 44689.DDB0233583; -.
DR   PaxDb; Q54N41; -.
DR   EnsemblProtists; EAL64598; EAL64598; DDB_G0285521.
DR   GeneID; 8625150; -.
DR   KEGG; ddi:DDB_G0285521; -.
DR   dictyBase; DDB_G0285521; isy1.
DR   eggNOG; KOG3068; Eukaryota.
DR   HOGENOM; CLU_043453_0_1_1; -.
DR   InParanoid; Q54N41; -.
DR   OMA; IPSCEKW; -.
DR   PhylomeDB; Q54N41; -.
DR   Reactome; R-DDI-6781823; Formation of TC-NER Pre-Incision Complex.
DR   Reactome; R-DDI-6782135; Dual incision in TC-NER.
DR   Reactome; R-DDI-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR   Reactome; R-DDI-72163; mRNA Splicing - Major Pathway.
DR   PRO; PR:Q54N41; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0071014; C:post-mRNA release spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0071020; C:post-spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR   GO; GO:0005681; C:spliceosomal complex; ISS:dictyBase.
DR   GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IBA:GO_Central.
DR   GO; GO:0000389; P:mRNA 3'-splice site recognition; IBA:GO_Central.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:dictyBase.
DR   Gene3D; 1.10.287.660; -; 1.
DR   InterPro; IPR029012; Helix_hairpin_bin_sf.
DR   InterPro; IPR009360; Isy1.
DR   InterPro; IPR037200; Isy1_sf.
DR   PANTHER; PTHR13021; PTHR13021; 1.
DR   Pfam; PF06246; Isy1; 1.
DR   SUPFAM; SSF140102; SSF140102; 1.
PE   3: Inferred from homology;
KW   mRNA processing; mRNA splicing; Nucleus; Reference proteome; Spliceosome.
FT   CHAIN           1..299
FT                   /note="Pre-mRNA-splicing factor ISY1 homolog"
FT                   /id="PRO_0000331195"
FT   REGION          223..252
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   299 AA;  35579 MW;  8CC611D92EB24F2F CRC64;
     MARNEEKAKS MLNRYLQLKG TESKQEERRP YLSNECDSLV DAEKWRRQIL KEITRGITEI
     QNSALDEYKI RDLNDHINKL VREKGHWERR ILQLGGPNHR ALAPKLFDAD GKEPLGTGTY
     RYYGEAKNLP GVAELFEKPE QTLHGTEKKT RQDLYKYIDS DYYGYRDDED GKLEEIEREY
     EKYAIEQNVE NWKKQQRDKF QINTNRFNNN NIKNSIAINK NTENKDEINI DDDEENDDNN
     NNNNNNNTEN TENIENIQEE EEKEDIKFKS YVSLPSTSQI ETILLEKRKE ELRKRYTQN