ID   ISY1_KLULA              Reviewed;         237 AA.
AC   Q6CJQ3;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Pre-mRNA-splicing factor ISY1;
GN   Name=ISY1; OrderedLocusNames=KLLA0F16841g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Involved in pre-mRNA splicing. {ECO:0000250}.
CC   -!- SUBUNIT: Associated with the spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ISY1 family. {ECO:0000305}.
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DR   EMBL; CR382126; CAG98544.1; -; Genomic_DNA.
DR   RefSeq; XP_455836.1; XM_455836.1.
DR   AlphaFoldDB; Q6CJQ3; -.
DR   SMR; Q6CJQ3; -.
DR   STRING; 28985.XP_455836.1; -.
DR   PRIDE; Q6CJQ3; -.
DR   EnsemblFungi; CAG98544; CAG98544; KLLA0_F16841g.
DR   GeneID; 2895655; -.
DR   KEGG; kla:KLLA0_F16841g; -.
DR   eggNOG; KOG3068; Eukaryota.
DR   HOGENOM; CLU_043453_2_1_1; -.
DR   InParanoid; Q6CJQ3; -.
DR   OMA; QWHIDHT; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0071014; C:post-mRNA release spliceosomal complex; IEA:EnsemblFungi.
DR   GO; GO:0071020; C:post-spliceosomal complex; IEA:EnsemblFungi.
DR   GO; GO:0000974; C:Prp19 complex; IEA:EnsemblFungi.
DR   GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; IEA:EnsemblFungi.
DR   GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; IEA:EnsemblFungi.
DR   GO; GO:0000384; F:first spliceosomal transesterification activity; IEA:EnsemblFungi.
DR   GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IEA:EnsemblFungi.
DR   GO; GO:0000389; P:mRNA 3'-splice site recognition; IEA:EnsemblFungi.
DR   Gene3D; 1.10.287.660; -; 1.
DR   InterPro; IPR029012; Helix_hairpin_bin_sf.
DR   InterPro; IPR009360; Isy1.
DR   InterPro; IPR037200; Isy1_sf.
DR   PANTHER; PTHR13021; PTHR13021; 1.
DR   Pfam; PF06246; Isy1; 1.
DR   SUPFAM; SSF140102; SSF140102; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Spliceosome.
FT   CHAIN           1..237
FT                   /note="Pre-mRNA-splicing factor ISY1"
FT                   /id="PRO_0000192970"
SQ   SEQUENCE   237 AA;  27933 MW;  7BF202272008708B CRC64;
     MSRNVNKANS VLVRYQELQA AETGGYQDFS RLKRPTRINK VTKLDEALRW RNELVKEIGQ
     RVTQIHDPSL NESQITEIND ELNKLFQEKG RWDFHIRNKL KGPDLKKRKQ LNTTGGTLID
     GKRYFGRALE LPEVQKLLRE SKEQREKERS GKEKKAELQR KIKRWESTLK DDYFVGNNSD
     ELLEYEKKRT GSLRSTIHSS DRDPSFVIPN FDVPSLKDVE LWLVQRRKNE LKKQLGL